Zeiss Axioscope 5, Инструкция по эксплуатации

Страница: 97 / 204

ZEISS 5 Operation | 5.4 Setting Up for Transmitted Light Techniques
5.4.6 Setting Up for Transmitted Light DIC Microscopy
Info
The DIC method works with polarized light. It is disturbed when birefringent elements, e.g.
foils, are put between polarizer and analyzer, as is sometimes done when doing a histological
incision. The same situation occurs with Petri dishes or sample holders which have a plastic
base. In these cases we recommend using the PlasDIC method.
1
2
7
3
4
5
6
Fig. 52: Setting up for transmitted light DIC microscopy
1 Reflector turret 2 Analyzer module in the reflector insert
3 Objective on the nosepiece 4 Condenser with DIC prism
5 Knurled screw for optimal contrast 6 DIC slider
7 Compensator λ
Prerequisite ü The microscope is operational.
ü Objective equipped with DIC fixtures, e.g. EC Plan-NEOFLUAR, is installed [
}
67] .
ü
Nosepiece with slot for DIC slider is installed.
ü DIC slider, compatible with the objectives in use, is available.
ü
Condenser with turret disk containing DIC prisms (e.g. condenser, achromatic-aplanatic 0.9 H
D PhC DIC) is installed.
ü The analyzer module ACR P&C for transmitted light in the reflector turret/slider or the analyzer
slider D/A fixed or rotatable in conjunction with a mounted intermediate plate for analyzer
slider 12x46 is installed.
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001 97