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1. Press the POWER button to turn the pump on.
2. Using the arrows, select CALIBRATE BIO-PUMP.
3. Connect the fl ow indicator or TSI Calibrator.
4. Press the SET button.
5. The pump will begin running at a default fl ow rate that is 

approximately 15 LPM. Use the arrows to adjust the fl ow 
rate until “15 LPM” registers on the fl ow indicator or on 
the TSI’s LCD. 

6. Press the SET key to save the calibration. 

Note

When you enter calibration mode, the pump will reset its fl ow rate to a default setting that
is approximately 15 LPM. To check the pump calibration without resetting the fl ow rate, 
you may use the “Verify Calibration” function.

CALIBRATE

BIO-PUMP

VERIFY

CALIBRATION

ADJUST FLOW THEN 

PRESS SET

Adjusting the Calibration

Verifying the Calibration

1. Press the POWER button to turn the pump on.
2. Use the arrow buttons to select VERIFY CALIBRATION.
3. Connect the fl ow indicator or TSI tube adapter.
4. Press the SET or START button to begin.
5. Measure the fl ow rate with the calibrator. To stop, press the stop 

button. To adjust the calibration, use the CALIBRATE BIO-PUMP 
option as described above. 

Calibration

It is recommended that the Bio-Pump

®

 Plus be calibrated and verifi ed at least once every day of use. 

A fl ow indicator for 

Air-O-Cell

®

 cassettes is supplied with the pump for ease of calibration. You may 

also calibrate your pump using the optional TSI-4046 Primary Calibrator with attachments. These are 
the only approved calibrators that are specially designed to work with low backpressure impeller fan 
type pumps.

Warning

DO NOT calibrate the pump using any other devices. Doing so will yield inaccurate readings due to 
the backpressure created by the calibrators. Calibrators known by 

Zefon

 to cause theses inaccuracies 

include, but are not limited to:

Bios Drycal, Bios Defender, Gilian Gilibrator, Gilian Challenger, or standard miniBUCK calibrators.

15LPM Mark

Float Ball

Adjust fl ow until ball is centered on line.

Connect fl ow indicator to pump as shown

4

Sampling Modes

Bio-Pump

®

 Plus comes with 3 different sampling modes to choose from:

Taking Sampling Using the Quick Sample Mode

Taking Samples Using Single Sampling Mode

1. Place the pump in the desired location.
2. Place cassette on the rubber grommet on top of the pump so that 

the cassette fi ts snugly.

3. Press the POWER button to turn the pump on.
4. Using the arrows, select “SINGLE SAMPLING MODE”
5. Press SET.
6. Using the arrows, enter the sample run time (adjustable in 1 

minute increments).

7. Press SET. (optional)
8. Press START to begin sampling.  Once completed, the unit will 

beep, the display will show “SAMPLE COMPLETE” and the yellow 
sample complete light will turn on. Remove cassette from pump 
by gently pulling upward.

9. Seal cassette, document the sample run time and send to 

laboratory for analysis.

Note

: “Single Sample” mode will exit upon hitting the “SET”        

button after runtime has been set. Previous runtime will be stored 
in memory.

1. Place the pump in the desired location.
2. Place cassette on the rubber grommet on top of the pump so that 

the cassette fi ts snugly.

3. Press the POWER button to turn the pump on.
4. Press the “QUICK SAMPLE” button. The display should read 

“Quick Sample, 1 minute. 

5. Continue to press the “QUICK SAMPLE” button to change the 

sampling time from 1, 2, 5, and 10 minutes.

6. Once the correct number of minutes is displayed, press “START” 

to begin sampling.

7. Once completed, the unit will beep, the display will show

 “SAMPLE COMPLETE” and the yellow sample complete light will 
turn on. Remove cassette from pump by gently pulling upward.
Seal cassette, document the sample run time and send to 
laboratory for analysis.

Note

: “Quick sample” mode will exit upon hitting the “SET” button 

at any time.

1. Quick Sample Mode

Quick Sample modes allows easy sampling of the most commonly used sampling times of 1, 2, 5, 
or 10 minutes.

2. Single Sampling Mode

Single Sampling Mode is designed to take one sample at a predetermined amount of time. The 
amount of sample time can be set anywhere from 1 minute to 9 hours 59 minutes.

3. Sequential Sampling Mode

Sequential sampling allows the pump to be programmed to turn on and off at predetermined times. 
Example: it can be programmed to run for one minute every hour for fi ve hours.

5

RUN TIME  0:00:00

SET TIME  0:00:00

ENTER RUN TIME

0:00:00

SINGLE

SAMPLING MODE

SAMPLE COMPLETE

RUN TIME  0:00:00

SET TIME  0:00:00

QUICK SAMPLE

5 MINUTE

SAMPLE COMPLETE

Select Quick Sample Time, then 

press START

Display shows set time and run time 

during sampling

Display shows set time and run time 

during sampling

Use Up/Down arrows to select Single 

Sampling Mode, then press SET

Use Up/Down arrows to enter the 

desired run time, then press SET

PRESS START

 TO RUN SAMPLE

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