35
25. See the list of acceptable values in table 6-2 below to interpret linearity readings.
a. Values that are ± 5% of specified tolerance limits indicate good membranes.
b. Values that are out of tolerance indicate an aging enzyme membrane.
c. If readings are out of tolerance limits, recalibrate and repeat all the steps above to confirm.
d. If the reading is still out of tolerance, refer to the Troubleshooting Section.
Chemistry
Calibration Std
Linearity Std
Acceptable Range (g/L)
Ammonium
2972 (0.50 g/L)
7179 (0.10 g/L)
0.095 to 0.105
Choline
2772 (0.175 g/L)
2773 (0.450 g/L)
0.43 to 0.47
Ethanol
2790 (2.00 g/L)
2790 (3.20 g/L)
3.04 to 3.36
Ethanol-HC
7151 (25.0 g/L)
7152 (40.0 g/L)
38.0 to 42.0
Glucose
2776 (2.50 g/L)
1531 (9.00 g/L)
8.55 to 9.45
L-Glutamate
2755 (0.73 g/L)
2756 (1.46 g/L)
1.39 to 1.53
L-Glutamine
2736 (0.73 g/L)
2737 (1.17 g/L)
1.11 to 1.23
Glycerol
7141 (25.00 g/L)
7142 (40.00 g/L)
38.0 to 42.0
L-Lactate
2776 (0.50 g/L)
1530 (2.67 g/L)
2.54 to 2.80
Lactose
2783 (5.00 g/L)
2784 (25.00 g/L)
23.75 to 26.25
Methanol
2726 (1.00 g/L)
2726 (2.50 g/L)
2.38 to 2.63
Potassium
2971 (1.00 g/L)
7179 (0.20 g/L)
0.190 to 0.210
Sucrose
2780 (5.00 g/L)
2778 (25.00 g/L)
23.75 to 26.25
Xylose
2767 (20 g/L)
2768 (30 g/L)
25.5 to 34.5
Table 6-2
Sample Preparation
5.2
A variety of sample types can be analyzed with the 2900 Series. Generally, the only sample preparation that
may
be
required is dilution of the sample to bring the substrate concentration within the linear range of the instrument. (see
Section 8 Chemistry Setup for the working range of each chemistry).
Neither color nor turbidity interferes with measurements.
Small particles do not affect the reaction in the sample module that houses the probes, but samples with particles large
enough to clog the sipper should be avoided.
Run Batch
5.3
5.3.1 Create Batches
1. From the Run Batch tab of the Run screen, select a sample rack/plate and create batches for your samples.
3
Range for single linearity check. Refer to
