6
World Precision Instruments
MAINTENANCE
There is no need to dis-assemble the pipette. Disassembly voids the warranty.
Calibrating the Pipette
Pipettes should be calibrated annually. The accuracy of the calibration depends
on the equipment used and your attention to detail. For best results, follow these
guidelines:
•
Use distilled water for calibration.
•
Make sure the pipette, tips, water and balance are all at the same room
temperature. Keep them in the same location for two hours prior to calibration.
•
Atmospheric pressure and room temperature can affect the calibration
measurements. Many calibration software packages are available. Using such
software allows you to correct for the environmental effects.
1. Set the pipette to the maximum volume. Place a disposable tip on the pipette.
Using a balance, measure the weight of three successive aliquots of distilled
water to the nearest 0.0001 of a gram.
2. Record each value and then average the three values.
3. Set the pipette to its minimum volume, and repeat steps 1–2.
4. Locate the calibration tool that came with your pipette. Clip it onto the pipette
shaft with the teeth pointed toward the pipette body. Then, line up the teeth
with the holes in the top of the adjustment dial.
Fig. 4—(Left) The calibration tool came with your pipette.
Fig. 5—(Center) Clip the calibration tool on the shaft of the pipette push button.
Fig. 6—(Right) Push the teeth on the calibration tool into the holes in the adjustment dial.
5. Rotate the calibration tool counter-clockwise to increase the aliquot dispensed
and clockwise to decrease it.
6. Repeat steps 1–3 until the minimum and maximum volumes are accurate.
Содержание REG10
Страница 3: ...iv World Precision Instruments...
Страница 13: ...10 World Precision Instruments...