1
[ CARE AND USE MANUAL ]
CONTENTS
I.
INTRODUCTION
II.
GETTING STARTED
a. eCord Installation
b. Column Installation
c. Column Equilibration
d. Initial Column
Efficiency Determination
e. Column QR Code
f. XSelect
XP
Columns
— VanGuard Protection
g. Replacing the
VanGuard FIT Cartridge
II.
COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
III.
COLUMN CLEANING,
REGENERATING AND STORAGE
a. Cleaning and Regeneration
b. Storage
IV.
eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
V.
ADDITIONAL INFORMATION
a. Tips for Maximizing ACQUITY
HSS Column Lifetimes
b. Recommended Flow Rates and
Backpressures for Reversed-Phase
ACQUITY HSS Columns
VI.
CAUTIONARY NOTE
I. INTRODUCTION
Thank you for choosing a Waters™ XSelect
XP
and/or XSelect
Premier HSS Column. The XSelect HSS packing materials
are manufactured in a cGMP, ISO 9001:2000 certified plant
using ultra pure reagents. Each batch of XSelect HSS material
is tested chromatographically with acidic, basic and neutral
analytes and the results are held to narrow specification ranges
to assure excellent, reproducible performance. Every column
is individually tested and a Performance Chromatogram
and Certificate of Batch Analysis are provided on the
eCord™ intelligent chip.
The XSelect Premier HSS Column is offered with or without a
VanGuard™ Fully-Integrated Technology [FIT] Cartridge.
To address the desire to extend the operating lifetimes of
analytical columns, the VanGuard FIT Cartridge is designed
to prevent the non-desired introduction of sample matrix or
particulates onto the column without degrading the separation.
It can be easily replaced to restore separation performance and
extend the analytical column’s lifetime.
In addition, the XSelect Premier HSS Columns utilize
MaxPeak™ High Performance Surfaces, an innovative
technology designed to increase analyte recovery, sensitivity,
and reproducibility by minimizing analyte/surface interactions
that can lead to sample losses.
XSelect
XP
and XSelect Premier 2.5 µm HSS Columns
