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4-4 Configuring the NanoLockSpray Source
NanoFlow gas supply
The nebulizer gas supply pressure for the sample sprayer is electronically
controlled from 0 to 2 bar. The optimum pressure is sprayer-dependent, but it
usually lies between 0.3 and 1.0 bar.
Purge gas
Purge gas, typically flowing at 100 L/hour, provides a positive pressure in the
source enclosure, reducing the chemical background interference caused by
contaminants in the laboratory air. You can adjust this flow in the software
(see the mass spectrometer’s online Help for details).
Sprayer platform adjuster assembly
The sprayer platform adjuster assembly allows precise X-, Y-, and
Z-positioning of the sprayer tip. You can also withdraw the sprayer from the
source to allow access to the sprayer tip.
Using the two thumbscrews on the base of the adjuster assembly, you can
move the platform in and out of the source (see page 4-6 ).
Configuring the NanoLockSpray source
The Universal NanoFlow sprayer is installed as standard equipment on the
NanoLockSpray source. For installation and maintenance details, see the
Waters Universal NanoFlow Sprayer Installation and Maintenance Guide
(part number 71500110107).
The following table summarizes how you configure the NanoLockSpray source
for the various ionization modes.
Tip:
A corona pin is not used with the NanoLockSpray source.
Caution:
Connect the UPLC column directly to the universal sprayer.
Do not connect via the diverter valve. The analyte section of the
IntelliStart Fluidics system is not designed for use at the flow rates
used in NanoFlow ESI.