[ CARE AND USE MANUAL ]
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
[ CARE AND USE MANUAL ]
Waters, The Science of What’s Possible, CORTECS, ACQUITY, UPLC, eCord, VanGuard, MaxPeak, Arc,
Oasis, Sep-Pak, Empower, and MassLynx are trademarks of Waters Corporation.
All other trademarks are the property of their respective owners.
2022 Waters Corporation. Produced in the U.S.A. December 2022 720005024EN KK-PDF
Injection Solvents
1. If possible, injection solvents should be 95% acetonitrile.
The polar solvent (i.e., water, methanol, isopropanol)
should be minimized to 25% of the total volume.
2. A generic injection solvent is 75:25 acetonitrile:methanol.
This is a good compromise between analyte solubility and
peak shape.
3. Avoid water and dimethylsulfoxide (DMSO) as injection
solvents. These solvents will produce very poor peak shapes.
4. Exchange water or DMSO with acetonitrile by using
reversed-phase solid-phase extraction (SPE). If this is not
possible, dilute the water or DMSO with organic solvent.
Miscellaneous Tips
1. CORTECS HILIC columns are designed to retain very polar
bases. Acidic, neutral, and/or non-polar compounds
will have limited retention.
2. Optimal flow rates for small (<200 daltons) very polar
bases are in the 0.4 to 0.8 mL/min range.
3. As compared to Atlantis™ HILIC Silica HPLC Columns,
the CORTECS HILIC columns are approximately 20%
less retentive for gradient analysis and 35–65% less
retentivefor isocratic analysis.
4. In HILIC, it is important to remember that water is the
strongest solvent. Therefore, it must be eliminated or
minimized in the injection solvent.
5. For initial scouting conditions, run a gradient from 95%
acetonitrile to 50% acetonitrile. If no retention occurs,
run isocratically with 95:3:2 acetonitrile:methanol:
aqueous buffer.
6. Alternate polar solvents such as methanol, acetone,
or isopropanol can also be used in place of water to
increase retention.
7. If using an ACQUITY UPLC System, the weak needle wash
should closely match the % organic present in the initial
mobile-phase conditions, otherwise, analyte peak shape
or retention may suffer.
8. It is recommended to monitor your systems health by
incorporating the HILIC Quality Control (QC) Reference
Material into your work flow. (Reference
720004535EN
).
VII. CAUTIONARY NOTE
Depending on the user’s application, these products may be
classified as hazardous following their use, and as such are
intended to be used by professional laboratory personnel
trained in the competent handling of such materials.
Responsibility for the safe use and disposal of products rests
entirely with the purchaser and user. The Safety Data Sheet
(SDS) for this product is available at
www.waters.com/sds
.