Waters CORTECS Скачать руководство пользователя страница 1

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[ CARE AND USE MANUAL ]

CONTENTS

I.  

INTRODUCTION

II. 

GETTING STARTED

 

a.  eCord Installation

 

b.  Column Installation

 

c.  Column Equilibration

 

d.  Initial Column 

 

  Efficiency Determination

 

e.  Column QR Code

 

f.  CORTECS Columns 

 

  — VanGuard Protection

 

g.  VanGuard FIT Cartridges

III. 

COLUMN USE

 

a.  Sample Preparation

 

b.  pH Range

 

c. Solvents

 

d. Pressure

 

e. Temperature

IV. 

COLUMN CLEANING, REGENERATING,  

 

AND STORAGE

 

a.  Cleaning and Regeneration

 

b. Storage

V. 

eCORD INTELLIGENT 

 

CHIP TECHNOLOGY

 

a. Introduction

 

b. Installation

 

c.  Manufacturing Information

 

d.  Column Use Information

VI. 

ADDITIONAL INFORMATION

 

a.  Tips for Maximizing CORTECS 

 

  Column Lifetimes

 

b.  Getting Started with CORTECS  

 

  HILIC Columns

VII. 

CAUTIONARY NOTE

I. INTRODUCTION

Thank you for choosing a Waters™ CORTECS™ and/or 
CORTECS Premier Column. The CORTECS packing materials 
are manufactured in a cGMP, ISO 9001 certified plant using 
ultra pure reagents. Each batch of CORTECS packing 
material is tested chromatographically with acidic, basic, 
and neutral analytes and the results are held to narrow 
specification ranges to assure excellent, reproducible 
performance. Every column is individually tested and a 
Performance Chromatogram and Certificate of Batch 
Analysis are provided on the eCord™ Intelligent Chip. 

The CORTECS Premier Column is offered with or without 
a VanGuard™ Fully-Integrated Technology [FIT] Cartridge. 
To address the desire to extend the operating lifetimes of 
analytical columns, the VanGuard FIT Cartridge is designed 
to prevent the non-desired introduction of sample matrix or 
particulates onto the column without degrading the separation. 
It can be easily replaced to restore separation performance and 
extend the analytical column’s lifetime. 

In addition, the CORTECS Premier Columns utilize 
MaxPeak™ High Performance Surfaces, an innovative 
technology designed to increase analyte recovery, sensitivity, 
and reproducibility by minimizing analyte/surface interactions 
that can lead to sample losses.

CORTECS and CORTECS Premier 2.7 

µ

m Columns

Содержание CORTECS

Страница 1: ...CORTECS packing material is tested chromatographically with acidic basic and neutral analytes and the results are held to narrow specification ranges to assure excellent reproducible performance Ever...

Страница 2: ...lumn outlet to the detector This prevents entry of air into the detection system 4 Gradually increase the flow rate as described in Step 2 5 Once a steady backpressure and baseline have been achieved...

Страница 3: ...guard holder and guard cartridge can be easily attached to the inlet of the column For more information on VanGuard products visit www waters com vanguard g VanGuard FIT Cartridges The VanGuard FIT C...

Страница 4: ...ating temperatures are recommended for longer column lifetime Working at high temperatures e g 45 C may also result in shorter column lifetimes III COLUMN USE To ensure the continued high performance...

Страница 5: ...at 10 mM or less Ammonia NH4 OH Ammonium bicarbonate 9 2 10 3 HCO3 9 2 NH4 8 2 10 2 8 2 11 3 Volatile Volatile Yes Yes Used in the 5 10 mM range for MS work keep source 150 C Adjust pH with ammonium...

Страница 6: ...rface charge modification End cap style Carbon load Ligand density pH limits Temp limits C18 Trifunctional C18 None Proprietary 6 6 2 7 mol m2 2 8 45 C C18 Trifunctional C18 Proprietary 5 7 2 4 mol m2...

Страница 7: ...rmate containing mobile phase V eCORD INTELLIGENT CHIP TECHNOLOGY May not be available for all column configurations a Introduction The eCord Intelligent Chip provides the history of a column s perfor...

Страница 8: ...ple set maximum pressure and temperature in the sample set and if the column met basic system suitability requirements Up to 50 sample sets can be stored on the eCord chip VI ADDITIONAL INFORMATION a...

Страница 9: ...related questions to ask If I inject neat standards prepared in mobile phase do I observe these problems If I prepare my standards in water and prepare them like samples e g SPE filtration etc do I s...

Страница 10: ...es are in the 0 4 to 0 8 mL min range 3 As compared to Atlantis HILIC Silica HPLC Columns the CORTECS HILIC columns are approximately 20 less retentive for gradient analysis and 35 65 less retentivefo...

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