To prime a wetted binary solvent manager:
1.
In the ACQUITY UPLC Console, click
Control
>
Wet Prime
.
2.
In the Wet Prime dialog box, review the settings and select a different option, if needed:
• Solvent line A only (default)
• Solvent line B only
• Both A and B
3.
Click
OK
.
Result:
The system primes the selected solvents, primes the sample manager with one
weak-wash prime (using the wash and sample syringes), sets the flow and temperatures,
and ignites the lamp in the MS.
3.4 Priming the system
Requirement:
You must prime the system after starting it, as well as after changing the mobile
phase, after changing the sample needle, and after the system is idle for four hours or more.
Recommendation:
If you are introducing new solvents, prime them at 4 mL/min for seven
minutes. Alternatively, prime the solvents at 4 mL/min for three minutes. Ensure that sufficient
quantities of solvent are available for priming.
Tip:
In the console, you can select the
Startup System
feature to prime all solvents and to
specify the solvent composition, flow rate, column and sample temperatures, and needle
characterization for your next system start-up. For details, see the console online Help.
3.5 Monitoring from control panels
You can monitor the sample manager, solvent manager, column module, detector, and fraction
manager from control panels, which you access via the chromatography data system.
• When Empower software controls the system, the control panels appear at the bottom of the
Run Samples window.
• When MassLynx software controls the system, the control panels appear on the
Additional
Status
tab of the Inlet Editor window.
• When UNIFI software controls the system, the control panels appear in the right-hand utility
pane of the main window whenever a system is selected for the System Console or data
analysis activities.
You can update a parameter set point directly from a control panel, providing sample analysis is
not running. When the parameter value is underscored and appears in blue, sample analysis is
not running, and you can click the parameter value and specify a new value in the dialog box that
appears.
April 4, 2018, 715005704 Rev. A
Page 48
