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The Application protocol

What is an Application protocol?

The software runs with pre-defined image acquisition parameters to facilitate the use of the system for a specific
application. These pre-defined parameters are gathered in the software Application protocol.

The Application protocol automates a task or a set of tasks that you perform repeatedly or on a regular basis. It stores
the following information:
- The image exposure pre-defined set-up;
- The live preview pre-defined parameters;
- The image display pre-defined set-up;
- The image printing pre-defined set-up;
- The image file pre-defined set-up.

The factory settings include several pre-defined Application protocols

You can also create your own Application protocol.

The benefits of the Application protocol are as follows:
- Time saving
- Reproduction of image acquisition parameters
- Protocols are modifiable, allowing the user to maintain an original template while modifying it for a slightly different
result, with minimal effort

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Содержание UVIDOC HD6

Страница 1: ...1 UVIDOC HD6 QUICK START USER GUIDE ...

Страница 2: ...your satisfaction and we will be pleased to answer any question you may have We are also very receptive to your suggestions Many of the new features and enhancements in this system are a direct result of conversations with our customers Please do not hesitate to contact us to let us know what you would like to see in the next version of this system You can contact us at the following addresses Ema...

Страница 3: ...mation 13 UVIDOC HD6 system installation 16 Unpacking the system 16 Installing the hardware 18 Environmental conditions 18 Imager back side 19 Operating instruction 21 Warning while using the darkroom 21 Imager front side Door open 22 Filter wheel 22 System operation 23 Introduction 23 Start the UVIDOC HD6 system software 24 Capture an image 25 Fluorescence image acquisition 25 Image capture workf...

Страница 4: ...s 46 General Image file pre defined parameters 47 The System settings 49 The general system settings 50 Windows parameters 51 Lock unlock the Uvidoc system 51 Add a printer 51 Set the network parameters 51 Connect to the Uvidoc from your computer 53 Connect using your internet browser 53 Connect using the Uvitec1D software 53 Update the Uvidoc HD6 firmware 56 Technical information 57 Electrical sp...

Страница 5: ...tly determine for themselves whether their activities infringe any valid patent Uvitec Ltd cannot be held responsible for patent infringement resulting by the inappropriate user activities All goods and services are sold subject to the terms and conditions of sale of Uvitec Ltd A copy of these terms and conditions is available on request Copyright 2004 2017 by Uvitec Ltd All rights reserved Tradem...

Страница 6: ...strument should be used only by trained personnel who know the health risks associated with the UV radiation normally associated with this instrument Users should be trained on the appropriate personal protection gear for working with UV light to minimize UV exposure About the UVIDOC imaging system The UVIDOC imaging system is a scientific instrument designed to capture fluorescence or colorimetri...

Страница 7: ...ur workshop or elsewhere and whether or not the faulty part is to be replaced all other freight charges incurred being at the cost of the purchaser Returned goods will not be accepted for repair unless previous written authorization is obtained from our service department A request for authorization must be accompanied by an itemized list of products model numbers and the corresponding invoice num...

Страница 8: ...nitions below WARNING Whenever you find this pictograph be sure to refer to this Manual WARNING indicates an imminently hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood UV WARNING Whenever you find this pictograph be sure to refer to this Manual UV WARNING indicates an imminent...

Страница 9: ...rials for which the UVIDOC imaging system has not been designed for WARNING Do not use the instrument if smoke atypical noises or odors can be perceived or if the instrument becomes unusually hot as this may result in fire or electric shock Stop using the instrument immediately turn off the power switch and unplug the instrument from the power outlet Contact Uvitec Ltd representative to request re...

Страница 10: ...s take all suitable protective measures such as wearing protective glasses and gloves resistant to the substances used Follow local and or national regulations for safe operation and maintenance of the system WARNING Do not use the instrument within or near a sink or in humid above 70 RH or dusty environments This can result in fire or electric shock WARNING As the equipment is heavy it cannot be ...

Страница 11: ...mask and gloves UV radiation can be dangerous for unprotected eyes and skin therefore we recommend the user to wear UV protective goggles LP 70 or face shield MP 80 or MP 800 Protect all skin surfaces including the neck ears and hands Use of the UV transilluminator acrylic protective screen does not guarantee the user protection from UV radiation The use of protective goggles mask and or gloves is...

Страница 12: ...io PRECAUCION La radiación ultravioleta puede ser peligrosa para los ojos y la piel expuestos sin protección Para protegerse es impérativo usar gafas o una máscara CONSERVAR CUIDADOSAMENTE ESTAS INSTRUCCIONES AVERTISSEMENT Lors de l utilisation de cet appareil le non respect des instructions peut provoquer de graves brûlures à l utilisateur ATTENTION Le rayonnement ultraviolet est dangereux pour l...

Страница 13: ...い皮膚にとって危険です使用者はUV保護眼鏡 Ref LP70 またはフェースシールド Ref MP 80またはMP 800 を装着する必要があります将来のためにこれらの説明書を保管しておいてください Important electrical power safety information WARNING Connect the UVIDOC imaging system to an appropriate AC voltage outlet that is properly grounded and protected by a circuit breaker Connecting to ground constitutes an obligatory protection WARNING Power down the system and disconnect the AC m...

Страница 14: ... power switch must always be easy to access The power cord with plug must always be easy to disconnect WARNING The UVIDOC imaging system has serviceable fuses which are located on the rear panel and are a part of the power entry module See the Maintenance chapter of this guide for information about replacing the fuses WARNING All the equipment connected to this instrument shall be certified accord...

Страница 15: ...mage the instrument Only turn off power in an emergency situation WARNING Electrical shock hazard All instrument repairs or modifications should be performed by service personnel authorized by Uvitec Ltd Do not open any covers or replace parts unless specifically stated in the user documentation ...

Страница 16: ...al 1 Remove carefully each component from the box To remove the UVIDOC HD6 from the box please use the belts which surround the system and designed for this purpose Do not handle the system by the camera or the camera support Remove their protective plastic cover Place the darkroom at its permanent location the door facing forward The cabinet has to be placed near the computer used for image acqui...

Страница 17: ...of 15 C to 25 C 20 C recommended and that the relative humidity not exceed 70 non condensing WARNING Ensure that all of the systems ventilation openings are free of interference Excessive heat build up in the instrument may effect performance or cause operational failure The UVIDOC HD6 system should be located away from water solvents or a corrosive material on a bench top that is dry and stable T...

Страница 18: ...anel operation free space required 50 cm Right left rear and top minimum free space required 10 cm Other conditions Do not install the equipment near a window and avoid direct sunlight We recommend attached blinds to nearby windows Do not install the instrument where it may get wet or flooded Do not install the instrument in a dusty environment Do not install the instrument in a place constantly o...

Страница 19: ...er outlet A grounded circuit capable of delivering the appropriate current and voltage is required for installation Electrical requirements is located on the imager label on the rear panel of the imager Switch on the main switch 2 to position 1 on If required switch off the main switch 2 to position 0 off The main switch provides power to all of the imager components 1 2 Electrical requirements ...

Страница 20: ...d to the build in computer mother board The camera USB cable is connected to the mother board USB3 port 4 alternative USB ports could be used A mouse and a Keyboard could also be connected to these ports For network connection a LAN network port is also available The USB port of the left side of the instrument 3 is designed to hold your USB key drive for image saving The imager on off switch 4 sta...

Страница 21: ... the UV filter If the filter is too hot it will damage your electrophoresis gel UV WARNING Wait for at least 20 second in the High position before reducing the intensity selector to Low UV WARNING If one or several tubes are off or used and in order to keep a better homogeneity we recommend to change the 6 tubes simultaneously ...

Страница 22: ...ator on off button 8 to switch on the transilluminator light while the door is opened The white light switch on automatically when the door is opened Filter wheel The Uvidoc HD6 main switch is located inside the darkroom The 3 positions filter wheel is equipped with the F590 M58 interferential filter supplied with the system for Ethidium bromide application The F590 Ethidium bromide filter is atta...

Страница 23: ...NING Do not operate UVIDOC HD6 system in any other way than described in the User Manual The UVIDOC HD6 instrument is a high end ultra sensitive scientific camera platform designed to extract the lowest level of detection from your protein or DNA sample Our superior sensitivity resolution and dynamics provide optimal performance across a large array of applications The standard imaging process is ...

Страница 24: ...eft hand side of the imager press the power button to the position I to turn it on After the computer has booted up completely the following messages are displayed until the Uvidoc HD6 system is ready for image acquisition When system is ready the start menu appears on the display ...

Страница 25: ...ormer state it emits fluorescent light The Uvidoc HD6 system separates the emitted light from the excitation light in order to obtain an optimum sample image According to the configuration the Uvidoc HD6 can accommodate several excitation and emission channels in the visible and UV area and is ideal for a large array of applications such as 1D DNA gel 1D protein colorimetric samples multiplexing s...

Страница 26: ...parameters adjustment The Application protocol automates a task or a set of tasks that you perform repeatedly or on a regular basis It stores the following information The image exposure pre defined set up The live preview pre defined parameters The image display pre defined set up The image printing pre defined set up The image file pre defined set up The benefits of the Application protocol are ...

Страница 27: ...The auto mode The start mode The exposure modes are gathered in the exposure mode menu The Live mode Live mode allows direct visualization of the image This mode enables you to adjust the aperture and the focus and to position your sample Note A live image means the image displayed is refreshed every 1 20th of a second This short exposure time or frame is adequate for a variety of white light samp...

Страница 28: ...t field of view roughly 20x20cm at full zoom out to a landscape field of view roughly 26x21cm at full zoom out The 3D Scan images your sample in real time and reconstructs the data to create live three dimensional model The 3D reconstruction provides direct information regarding the image dynamic background level and protein or DNA quantity A little change of exposure time will refresh the 3D view...

Страница 29: ...s The Auto mode The Uvidoc HD6 system calculates automatically the optimum exposure time Auto exposure When you select the Auto Exposure option the system samples the light levels and uses the values to calculate the final exposure time To set the auto exposure mode select Auto from the exposure mode menu The Start mode The Uvidoc HD6 system requires the user to specify the exposure time To set th...

Страница 30: ... When the specified exposure time is reached the last captured image is displayed The camera continues to integrate the image on the sensor updating the display whenever the specified Exposure time is reached The Stop exposure button stops the exposure process The last full exposure is displayed You can also modify the zoom and focus settings display the 3D image and the saturation More details ar...

Страница 31: ... levels between white and black The image dynamic range within this scale Adjustments are necessary to obtain an image with the broadest dynamic With most of the gels a real time image has a limited dynamic In this case the exposure time enhances the image dynamic In order to obtain an optimum image you can control the image dynamic by adjusting the exposure time Click on Stop to finalize the imag...

Страница 32: ...The default image name has 8 characters The first 2 are IM The last 6 are a number ascending from 000001 to 999999 Note The default file name is incremented by one each time an image is saved Note The file extension varies according to the image file format For TIFF the extension is TIF For BMP file the extension is BMP For JPEG the extension is JPG To print press the Print key The image is printe...

Страница 33: ...doc HD6 images have 16 bit format and Windows can only display 8 bit images 256 grey levels Due to this limitation the software handles two images A memory image corresponding to the 16 bit format 65 536 grey levels A displayed image corresponding to the image displayed on the screen 256 grey levels The easiest way to calculate the display image would be to translate the full grey scale each time ...

Страница 34: ...alue defined will be converted to 0 Black in the displayed image All the grey levels upper the Max Value defined will be set to 255 White in the displayed image The grey levels between those two limits will be converted in an intermediate grey level value following a linear rule You can select one from the 5 image display presets by clicking on or sign ...

Страница 35: ... 1 Default optimum display Case 2 Optimum display enhancement The image appears brighter The faint bands are more visible Note The Display function inform you of the minimum and of the maximum grey level obtained on the image Note The Display has no impact on the analysis Only the display of the image is modified ...

Страница 36: ... following information The image exposure pre defined set up The live preview pre defined parameters The image display pre defined set up The image printing pre defined set up The image file pre defined set up The factory settings include several pre defined Application protocols You can also create your own Application protocol The benefits of the Application protocol are as follows Time saving R...

Страница 37: ...on the application name to open the application protocol menu The Application protocol can either be displayed with a summary view or with a complete view By default the application protocol is displayed with the summary view Click on Advanced options to have access to all the application protocol features ...

Страница 38: ...38 Advanced exposure Exposure predefined set up The Advanced Exposure folder contains the exposure pre defined set up Typical Exposure set up for a fluorescence application ...

Страница 39: ... Standard Big size 1 200x200mm 270x200mm 2 180x180mm 250x180mm 3 160x160mm 220x160mm 4 130x130mm 170x130mm 5 100x100mm 130x100mm 6 80x80mm 110x80mm Lighting Fluorescence mode only This option defines the lighting used for the image exposure All Uvidoc systems are equipped with LED Visible light You can select between low and high intensity Note The lighting options depend on your system configurat...

Страница 40: ...imum and maximum acceptable grey levels and calculate the exposure time so the highest grey levels values of the final image reach a maximum defined Saturation Value Note in the case the au exposure does not give you entire satisfaction for your specific application we recommend increasing the test time Auto mode The Auto mode options allow you to select between a standard auto exposure time an ex...

Страница 41: ... settings For instance 150 will increase the standard exposure time by 1 5 Manual exposure set up Manual exposure set up This option defines the default imaging exposure time The software has two exposure time scales One for short times 40 milli second to 4 seconds One for long times 4 sec to 1 minute Note With the short integration time scale the integration time increases or decreases by 40milli...

Страница 42: ...mera native image resolution by software interpolation Note This process requires important computer calculation and sometime could be necessary before the extended image is displayed The lens prefixed zoom position table The default positions are part of the system factory settings Nevertheless you can save your owned position using the zoom menu you can access during image acquisition ...

Страница 43: ...ble summarizes the default focusing preset per zoom option for the concerned Application protocol when modified by the user Display Image display pre defined parameters The Display folder contains the image display pre defined set up ...

Страница 44: ...le compared to their background This is well adapted for fluorescence sample Set the gamma value Gamma adjustment corrects an image by creating a new version of the original To create the new image the Gamma Adjust function reassigns the grey values of each pixel in the image according to the curve in the following graph The above graph demonstrates the basic principles of gamma adjustment Black p...

Страница 45: ...s software The saturation option allows you to visualize in red pixels that have the maximum grey level in order to avoid flattened peaks The maximum grey level depends of the bit depth For instance A 16 bit image has a maximum grey level of 65535 Select the saturation option and the saturated pixels are displayed in red Note If an image is being acquired and the Saturation option is checked the m...

Страница 46: ...Print with date and time Print the image with the computer time Print out text color Select the color of the text printing Free text print out You can select a free text for instance the name of your institute the image name if the image has been previously saved and date and time The text is then displayed below the image in the print out TUESDAY APRIL 27 2017 7 05AM ...

Страница 47: ...ation Protocol s avatar To simplify the reconnaissance of the Application s protocol an icon is provided to differentiate the application by a color Default file name Image name prefix The default file name could either be based on the Date and Hour the image has been taken or by a prefix You can decide of the default generic file name to simplify the file name generation By default the name is IM...

Страница 48: ...e the image is saved by default Alternatively you can select to always open the last used directory by clicking on the following option Autosave Use the Image Auto Save feature to automatically save images as part of the capture process in the defined image saving directory Preferred settings saving directory This option defines the directory where are saved the configuration files Select to autom...

Страница 49: ... The System settings Settings feature allows the adjustment of the system settings such as the filter controls or the lens calibration Click on Settings from the menu A window displays the following menu ...

Страница 50: ...em Shutdown the system The general system settings The General header from the Settings menu summarizes the information concerning your system and its set up It gives you access to the following information Systems name version Camera and darkroom state Options installed Calibration stat To proceed select the General header from the Settings menu Language selection The software could run in Englis...

Страница 51: ...ss network device With the Unlock function the write protection is disabled after the system is restarted At that time you can install the needed drivers Upon reboot the system will have the new drivers installed and will be write protected again Before to add a device it is compulsory to scan for virus the USB key drive which will contain the driver to be installed in the Uvidoc system Add a prin...

Страница 52: ...P IP is the most popular network protocol and the basis for the Internet In Windows operating system TCP IP is automatically installed On a TCP IP network you must provide IP addresses to clients This means you need to provide an address to make sure your Uvidoc is already properly connected to your LAN and that an IP address is assigned to the Uvidoc To proceed select the Control Panel function B...

Страница 53: ...connect your Uvidoc to the local area network of your laboratory and download your gel image from the comfort of your office The Uvidoc has a unique IP address which allows you to connect the system to your laboratory network for downloading The image acquisition is performed in your laboratory and the image is saved in the internal memory of the Uvidoc Then from your office you can easily downloa...

Страница 54: ...st be configured to permit users to request the specific Uvidoc IP address Connections are created and configured thanks to the Uvitec 1D IP address For remote access to the Uvidoc from your computer you need to set the Uvidoc IP address in the setup Uvitec 1D software To proceed click on Define connection parameters A pop up window displays the following menu Uvidoc HD6 IP address By default we r...

Страница 55: ...sword for your ftp access The password is then Uvidoc Connect to the Uvidoc To download the image from the Uvidoc click on Connect to the Uvidoc unit button A pop up windows prompt you the system is connecting to the Uvidoc The list of the Uvidoc images appears in the table Select your image An image thumbnail appears Click on Download selected image s to download your image and open it in the Uvi...

Страница 56: ...6 Then follow the Uvidoc system update procedure using the settings function Configuration settings windows parameter Software update Select the USB stick directory and the Setup exe file then click on OK Do not remove the USB stick or switch off the system till the process is finalized The process could last roughly 5 minutes For Editing or Analysis Please go directly to page 70 of this manual ho...

Страница 57: ...A 0 5A Fuse FST A 2A Voltage V 100 230V 5 Frequency Hz 50 60Hz Power 85 watts Fuses Type FST Time lag T Ø5 x 20 2A Climatic conditions Altitude 2000 meters Operating humidity 20 to 70 no condensation allowed Operating temperature The maximum ambient temperature should be 25 C ...

Страница 58: ...se Ø 5x20 Filter with support Qty 100V 115V 230V Qty Réf UVIdoc HD6 TOUCH 2 2 2A UVIdoc HD6 20M TOUCH 2 5 2 2A 1 FS TC20 CM UVIdoc HD6 26M TOUCH 2 5 2 2A 1 FS TC26 CM UVIdoc HD6 26MX TOUCH 2 5 2 2A 1 FS TC26 MX UVIdoc HD6 26LM TOUCH 2 5 4 2 2A 1 FS TC26 CM UVIdoc HD6 20Blue TOUCH 2 2 2A 1 FS B All our units are fitted with one or two safety fuses They are found in the plug at the rear of the unit ...

Страница 59: ...original spare parts not recommended by our service department is at the user s own risk and therefore automatically invalidates the warranty Tubes filters batteries and consumable products are not included in the warranty We reserve the right to decide where the faulty goods will be repaired in our workshop or elsewhere and whether or not the faulty part is to be replaced all other freight charge...

Страница 60: ... UVITEC LTD is not responsible for any injury or damage caused by use of this instrument for purposes other than those for which it is intended or by modifications of the instrument not performed by UVITEC LTD France only Decontamination collection and elimination of waste The buyer ensures and finances the decontamination the collection and the disposal of waste electrical and electronic equipmen...

Страница 61: ...lity and low voltage The Electro magnetic susceptibility has been chosen at a level that gains proper operation in residential areas on business and light industrial premises and on small scale enterprises inside as well as outside of the buildings All places of operation are characterised by their connection to the public low voltage power supply system ...

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