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HYDROGEN SENSOR USER MANUAL

UNISENSE A/S

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Содержание HYDROGEN SENSOR

Страница 1: ...1 HYDROGEN SENSOR USER MANUAL ...

Страница 2: ...Hydrogen sensor user manual Copyright 2016 Unisense A S Version December 2016 ...

Страница 3: ...HYDROGEN SENSOR USER MANUAL UNISENSE A S ...

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Страница 5: ...VIEW 9 GETTING STARTED 10 Unpacking a new sensor 10 Polarization 10 Connecting the microsensor 10 Pre polarization 10 Calibration 11 Hydrogen reading 11 MEASUREMENTS 14 Mounting of the sensors 14 Electrical noise 14 Interference 15 ADVANCED USE 16 STORAGE AND MAINTENANCE 17 Cleaning the sensor 17 REFERENCES 18 TROUBLE SHOOTING 19 ...

Страница 6: ...event will Unisense A S be liable for any direct indirect consequential or incidental damages including lost profits or for any claim by any third party arising out of the use the results of use or the inability to use this product Unisense mechanical and electronic laboratory instruments must only be used under normal laboratory conditions in a dry and clean environment Unisense assumes no liabil...

Страница 7: ...m Unisense A S Tueager 1 DK 8200 Aarhus N Denmark Tel 45 8944 9500 Fax 45 8944 9549 Further documentation and support is available at our website www unisense com CONGRATULATIONS WITH YOUR NEW PRODUCT REPLACEMENT OF SENSORS Unisense will replace sensors that have been damaged during shipment provided that The sensors were tested immediately upon receipt in accordance with the delivery note and the...

Страница 8: ...8 RECOMMENDED AMPLIFIERS One channel amplifier Microsensor Monometer Multi channel amplifiers Microsensor Multimeter ...

Страница 9: ... where the anode is polarized against the internal reference Driven by the external partial pressure hydrogen from the environment will pass through the sensor tip membrane and will be oxidized at the platinum anode surface The picoammeter converts the resulting oxidation current to a signal THIS MANUAL COVERS THE UNI SENSE HYDROGEN MICROSENSORS H210 tip diameter 20 µm H225 tip diameter 20 30µm H2...

Страница 10: ...osensor Connect the LEMO connector to the input terminal The LEMO connector contains connections for both internal reference elec trode and sensing anode Pre polarization Just after connecting the sensor the signal will be very high and unstable then drop rapidly over the first few minutes After that the signal will drop slowly for up to 1 hour Therefore a period of polarization is necessary befor...

Страница 11: ...drogen concentration which is slightly above the maximum expected hydrogen to be measured A defined hydrogen concentration can be obtained by 2 different procedures 1 Use a gas mixture controller to obtain a defined mixture of hydrogen and hydrogen free inert gas from a gas tank e g N2 as bulk carrier gas For instance to obtain a hydrogen concentration of 40 25 µM in the calibration chamber at 20 ...

Страница 12: ...volume of hydrogen saturated water to a defined volume of water in a calibration chamber For instance 1 ml of H2 saturated water contains 0 805 µmol at 20 C see Table 1 and to obtain water with a hydrogen concentration of 10 µM 3 08 ml hydrogen saturated water should be added to a total volume of 246 9 ml hydrogen free water in the calibration chamber After the addition of hydrogen saturated water...

Страница 13: ...nts can be calibrated to correct concentrations When the sensor is new the appropriate interval may be every 30 minutes later it may be 24 hours To minimize the need for calibrations keep the sensor polarized between measurements unless the time between measurements exceeds several days or unless the picoammeter batteries are running out The membrane permeability of hydrogen microsensors changes w...

Страница 14: ...crosensor tip and to the steepness of gradients in many environments even a displacement of the sensor tip of few microns may change its environment Therefore we recommend that measurements should be performed only in a stabilized set up free of moving or vibrating devices We recommend the Unisense lab stand LS and the Unisense micromanipulator MM33 MM33 2 or MMS for laboratory use For in situ use...

Страница 15: ...ensitivity to sulfide gas can be as much as 10 of the hydrogen sensitivity In addition exposure to high sulfide gas concentration can affect the calibration of the hydrogen sensor Therefore measurements done in environments with high sulfide concentrations are not recommended ...

Страница 16: ...n limit If your specifications for a special hydrogen sensor is not described at our web page please contact sales unisense com for further options and prices Examples of advanced applications Consumption production rates of hydrogen E g during enzyme assays in small samples in Unisense microrespiration chambers MRCh Measurements of hydrogen under high external pressure e g in closed pressurized s...

Страница 17: ...t 10 30 C If the sensor is used regularly it can be stored polarized Cleaning the sensor Depending on which substance is present on the sensor tip or membrane the sensor can be cleaned with different solutes The standard method is to rinse with 96 ethanol NOT in the protection tube then rinse with 0 01 M HCl and rinse with water This will remove most substances Alternatively it is possible to rins...

Страница 18: ...ys Res Commun 389 651 656 Kajiya M et al 2009 Hydrogen From Intestinal Bacteria Is Protective for Concanavalin A Induced Hepatitis Biochemical and Biophysical Research Communications 386 316 321 Kajiya M et al 2009 Hydrogen Mediates Suppression of Colon Inflammation Induced by Dextran Sodium Sulfate Biochemical and Biophysical Research Communications 386 11 15 Vopel K et al 2008 Modification of Se...

Страница 19: ...bration procedures when the signal is stable again Problem High and drifting signal Possible cause The sensor tip is broken Solution Replace the hydrogen microsensor Problem The signal is very low Possible cause Loss of the platinum tip due to excessive vibrations Solution Replace the hydrogen microsensor Problem Slow response Possible cause Insoluble compounds deposited at the sensor tip Solution...

Страница 20: ... sensor to high partial pressures of hydrogen 10 atm for at least 10 minutes to reactivate the sensor Problem Unstable signal or the signal fluctuates if the set up is touched or equipment is being introduced in the medium you are measuring in Possible cause Electrical disturbance of the sensor through the tip membrane Solution Ground the set up using the blue grounding cable supplied with the pic...

Страница 21: ... 34 831 70 789 29 780 80 772 77 764 73 756 70 748 66 6 912 95 866 96 823 21 781 70 773 66 765 63 757 59 749 55 741 96 8 893 30 849 11 807 14 767 41 759 38 751 79 744 20 736 61 729 46 10 875 45 833 04 792 41 754 02 746 43 739 29 731 70 724 55 717 41 12 858 93 817 86 779 02 741 96 734 82 727 68 720 54 713 39 706 70 14 843 75 804 02 766 52 730 80 723 66 716 96 710 27 703 13 696 43 16 829 46 791 52 75...

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Страница 24: ...UNISENSE DENMARK www unisense com info unisense com ...

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