10-AU Calibration for Chlorophyll
Страница 1: ...10 AU Calibration for Chlorophyll...
Страница 2: ...3 Main Steps 1 Setting the basic operating level 2 Blanking the instrument 3 Calibration with a liquid primary standard that has a known concentration...
Страница 3: ...rmed the 1st time you use the instrument or any time you switch to a different application change filters or lamps 1 Turn on the 10AU and allow the instrument to warm up for at least 30 minutes 1 Sett...
Страница 4: ...m 40 8 0 6 0 3 5 2 5 Medium 30 6 0 4 0 2 5 1 5 Medium 20 2 4 1 8 1 0 0 75 Low 80 1 8 1 6 0 75 0 65 Low 70 1 6 1 3 0 65 0 55 Low 60 1 3 1 1 0 55 0 45 Low 50 1 1 0 8 0 45 0 35 Low 40 0 8 0 6 0 35 0 25 L...
Страница 5: ...3 Prepare your standards and acetone blank in test tubes so you are ready to set the sensitivity and calibrate the instrument Be sure to clear past calibrations on screen 2 6...
Страница 6: ...4 Access screen 2 43 and set range control to manual and range to the appropriate level for your standard For our example the range should be set to MED on Screen 2 42 Change to MAN...
Страница 7: ...4 Access Screen 3 2 5 Loosen the sensitivity locking screw using allen wrench...
Страница 8: ...ert and cover your 20 g L standard 7 Using the sensitivity knob slowly turn the knob to adjust the FS to the value appropriate for your standard s concentration A deviation 5 is acceptable Sensitivity...
Страница 9: ...8 When the FS reaches the designated value tighten the sensitivity locking screw For our example this is 80 of medium The Basic Operating Level is now set and you can remove the standard for the 10 AU...
Страница 10: ...Setting the Basic Operating Level aka Sensitivity of your 10 AU is now complete You can access Screen 2 43 again and return range control to AUTO and begin calibration...
Страница 11: ...he sensitivity is set you should blank the instrument 1 Access screen 2 1 2 Insert and cover your blank acetone Press 1 to Run blank You can also choose to have the 10 AU subtract the blank signal fro...
Страница 12: ...Wait for the readings to stabilize and press 0 Wait for 15 seconds for the instrument to blank If Blanking is successful you will receive a Blank Finished message...
Страница 13: ...ndard Now that the blank is set you should calibrate with your standard 1 Access screen 2 0 2 Press 2 to access screen 2 2 and input the actual concentration of your standard Return to screen 2 0 3 In...
Страница 14: ...4 Press 3 to run standard Wait for the reading to stabilize Press the key and the calibration point is set Wait 15 seconds and you should receive a FINISHED message...
Страница 15: ...You can test check your calibration by running your standards and other samples or standards I ran the 50 g L and got I ran the blank and got I ran a 10 g L standard and got...