OTKBFM-CAL Calibration and Measurement Module for OTKB with OTKBFM User Guide
Страница 1: ...OTKBFM CAL Calibration and Measurement Module for OTKB with OTKBFM User Guide...
Страница 2: ...5 3 2 2 PSD Roll Off Method 5 Chapter 4 Setup 6 Chapter 5 Operation 10 5 1 Position Calibration 10 5 2 Stiffness Calibration 12 5 3 Sample Preparation 13 5 4 Saving Data 13 Chapter 6 Frequently Asked...
Страница 3: ...vice Symbol Description Direct Current Alternating Current Both Direct and Alternating Current Earth Ground Terminal Protective Conductor Terminal Frame or Chassis Terminal Equipotentiality On Supply...
Страница 4: ...trical shock CAUTION Caution is given when there is a possibility of damage to the product WARNING Given when there is danger of injury to users 2 2 General Warnings WARNING If the product is used in...
Страница 5: ...pproaches the most common technique is based on back focal plane detection As the laser beam passes through the sample plane interference occurs between the light which is transmitted through the trap...
Страница 6: ...cation of the trap while recording the detector voltage Using the sample stage steps of known size are used which then allow plotting of the position signal in volts versus the position signal in micr...
Страница 7: ...should be noted that for this approach it is necessary to first determine the position calibration for the detector 3 2 2 PSD Roll Off Method While the Roll Off method also makes use of the Brownian...
Страница 8: ...inistrative user rights for the installation 2 The Thorlabs APT software should already have been installed on your PC during the installation of the OTKBFM force module If not please download the APT...
Страница 9: ...lect Settings in one of the piezo controller windows and adjust the settings as shown in Figure 4 Close the window and do the same for the second piezo controller 7 Click Zero on the strain gauge read...
Страница 10: ...WARNING If the Piezo Driver is switched to closed loop mode without a feedback signal applied the piezo drive voltage will ramp up to the maximum limit If the limit is set to 75 V a protective circuit...
Страница 11: ...automatically added to your PC if it was not present already Start the NI MAX tool typically a link is found in your windows All Program list Right click on the NI USB 6212 device and rename it to OTK...
Страница 12: ...traints with the coverslip will restrict the Brownian motion while at heights above 5 m the optical trap loses its tight focus We assume that you have setup your tweezers system and aligned the detect...
Страница 13: ...trap position until you see a significant change in the voltage signals during the state oscillation Hint The stuck bead is aligned with the optical trap center when the voltage signal changes most s...
Страница 14: ...cted from the detector during a force calibration allowing the user to verify the result 1 Find a free bead and trap it 2 Use the data recording tool to verify that the X and Y voltages of the quadran...
Страница 15: ...one sphere for the duration of a measurement 5 4 Saving Data If the Stream to Disk flag is set on the Data Tracking screen a set of files will be saved Files can be named automatically using a time s...
Страница 16: ...ower cycle the K cube hub Then identify the K cube piezo and strain gauge controller pairs connected to the X and Y axis on the stage Set the piezo cubes to Open Loop temporarily and adjust the piezo...
Страница 17: ...OTKBFM CAL module d Check the PSD K Cube is set to Monitor Mode as shown in Fig 4 Use the Thorlabs APT User software to access the panel shown and adjust this setting 5 When I start data tracking in...
Страница 18: ...male XY Detector Signal Inputs 10 to 10 V SUM Detector Input 0 to 10 V XSG YSG Strain Gauge Inputs 0 to 10 V DAC Resolution 16 bit Sampling Range 400 kS s channel Outputs DAQ Manufacturer and Item Num...
Страница 19: ...Calibration Measurement Module for OTKB OTKBFM Chapter 8 CE compliance Page 17 Rev C August 2 2018 Chapter 8 CE compliance...
Страница 20: ...ther Thorlabs products such as Pure OEM products that means assemblies to be built into a unit by the user e g OEM laser driver cards Components Mechanics and optics Left over parts of units disassemb...
Страница 21: ...ww thorlabs de Email europe thorlabs com Scandinavia Thorlabs Sweden AB Bergfotsgatan 7 431 35 M lndal Sweden Tel 46 31 733 30 00 Fax 46 31 703 40 45 www thorlabs com Email scandinavia thorlabs com Fr...
Страница 22: ...www thorlabs com...