Optical Trap Kit
Manual
Страница 1: ...Optical Trap Kit Manual...
Страница 2: ...ety 3 2 Introduction 4 3 Setup Description and Segments 4 4 Optical Trap Kit Components 8 5 Force Measurement Module OTKBFM 11 6 Setup and Alignment in Detail 12 7 Software Package 24 8 Sample prepara...
Страница 3: ...this will result in damage to the controllers Set TEC and max LD current parameters according to the specifications of the trapping laser diode before operating the Optical Trapping Kit Failure to do...
Страница 4: ...e system allows you to adapt it for a wide variety of applications All of the components needed to construct a fully functional optical trapping system are included in this cost effective kit OTKB con...
Страница 5: ...nt the Camera segment and the Vertical segment all connect to this at its three ports as shown in Figure 5 2 Remember to screw the CPB1 to the table as indicated in picture After connecting the Collim...
Страница 6: ...eight is right Slide the four ER1 5 rods with vertical post base sliding also into C6W just enough such that the rods don t interfere with the rotation of the B4C in the cube Use the four set screws o...
Страница 7: ...gment mainly has the CCD camera for live imaging The imaging lens is already connected to the camera port on the cube C6W To complete this segment just slide the four ER1 rods into the four cage rod h...
Страница 8: ...is important to maintaining a constant trapping force 2 The FiberPort Item PAF X 7 B collimates the output of the trapping laser The FiberPort is a versatile collimator since it allows the aspheric c...
Страница 9: ...Optical Trap Kit 19590 D04 V15 Apr 12 2012 Page 9 www thorlabs com 5 3 4 8 2 7 9 Figure 3 2 Model of Thorlabs Optical Trapping system 10...
Страница 10: ...TPZ001 are included in the kit Two T Cube Strain Gauge Item TSG001 are included with the OTKBFM force module 8 The visible light emitted from the LED passes through the dichroic mirror and illuminates...
Страница 11: ...am axis As a result the output of the detector can be used to calibrate the position stiffness and force of the optical trap The detector is connected to the cage cube above the condenser as in Figure...
Страница 12: ...to put the Type 1 PCB card into the LM14S2 mount please refer to the LM14S2 manual for details 2 Mount the laser diode in the butterfly controller mount and connect to the laser diode controllers LDC2...
Страница 13: ...e desired beam centration is obtained Do not force the screws past their normal operating range if collimation is not easily achieved please contact Tech Support for assistance F Ensure that the beam...
Страница 14: ...that external retaining rings on the SM1V10 are on both sides of CP02 Make sure that the lens is mounted in the lens tube so that the collimated beam from the FiberPort is incident on the curved surfa...
Страница 15: ...y then you can put segments of the lens cover SC1L24 not included over the two open sections in Figure 6 2 1 You will need a two inch long cut for the first section and a one inch long cut for the sec...
Страница 16: ...an iris SM1D12D to this left end port of the C6W Then rotate the B4C on the C6W until the beam is going through the iris when it is almost closed and through the center hole of the VRC4CPT Then use th...
Страница 17: ...the KCB1 is used to align the beam through the upper diaphragm Neither should require much adjustment First the lower iris is wide open Then you gradually close this and if necessary use the kinematic...
Страница 18: ...from four ER05 cage rods 4 Place an LCP02 on a flat surface and insert the four ER05 cage rods in the 30 mm cage rod holes and secure them at such a level that only one of the two set screws in each...
Страница 19: ...adjusters on the HPT1 are oriented as shown in Figure 6 4 2 11 Attach an SM1D12 to the XY translating face of the HPT1 This face has a line marker for each adjuster The condenser will be connected to...
Страница 20: ...e slide 17 Lock both C1500 to the DP14 as shown in Figure 6 5 1 18 Use the other two ER6 rods to complete the upper vertical segment as shown in Figure 5 5 1 right 19 If you don t have to install the...
Страница 21: ...ssembly is shown in Figure 5 6 1 4 Mount the IR filter FES0750 in an SM1L05 5 Thread in an SM1D12 to the vertical face of the KCB1 then the SM1L05 from step 4 then an SM1T2 and the adapter SM1A9 which...
Страница 22: ...immersion objective might push the slide from the bottom during the imaging process the clips are used to hold the slide in place 6 8 The Light Source 1 Remove one half of the SM1Q quick release adap...
Страница 23: ...de of the connector on the cable which is marked with a 1 will connect to pin 6 please see LD1255R manual 7 Now gradually turn the Current Control Potentiometer and the LED should light up after a few...
Страница 24: ...lane Review of Scientific Instruments 77 103101 2006 The screen shots on the right hand side show the QPD and Strain Gauge Controller software on top included with OTKBFM and the piezo controller soft...
Страница 25: ...Following parts will be helpful to setup and operate the trap They are not part of the Optical Trap Kit a Immersion Oil e g Cargille non drying immersion oil for microscopy Type B Cat No 16484 Immers...
Страница 26: ...xis Sample Positioning Stage CCD Camera with USB Interface for Video Imaging Position Sensing Detector Module Available Specifications Trap Force 1 pN with Trap Laser Output Power 16 mW Spot Size 1 1...
Страница 27: ...4440 Fax 44 0 1353 654444 www thorlabs de email sales uk thorlabs com France Thorlabs SAS 109 rue des C tes 78600 Maisons Laffitte France Tel 33 0 970 444 844 Fax 33 0 811 381 748 www thorlabs de emai...
Страница 28: ...Thorlabs Inc 435 Route 206N Newton NJ 07860 USA Phone 973 579 7227 Fax 973 300 3600 www thorlabs com...
