Troubleshoot verification failure
Observation
Possible cause
Recommended action
Verification failed
The plate was improperly
prepared.
Ensure the following:
• The correct plate was used for the
verification performed.
• The plate was properly thawed.
• The plate was properly centrifuged.
• The plate was properly sealed.
Open the data file for the verification in the
desktop software or Thermo Fisher
™
Connect
to view the flags and troubleshooting details for
failed wells.
The plate is damaged or
contaminated.
Check for damage, improper plate seal, or
contamination.
Order a replacement plate. If the replacement
plate fails, contact Support.
High fluorescence signal
The reaction volume is not
correct.
Ensure that reaction volumes in the plate are
correct and match the volume that is entered in
the Method tab.
Signals that exceed the
limit of normal fluorescence
may indicate fluorescent
contaminants on the plate or the
sample block.
Examine the bottom of the reaction plate. If
there is contamination, prepare a new plate and
run the experiment again.
Identify the location of contamination on the
plate or sample block.
1. Obtain or prepare a background plate (see
2. Follow the procedures that are described in
Verification failed but plate is
undamaged
The incorrect plate was used for
verification.
Use the correct RNase P plate for verification.
The plate was improperly
prepared.
Repeat the verification with a new properly
prepared plate.
Note:
The verification procedure is an
experiment run, so each RNase P plate can only
be used once.
Open the data file for the verification in the
desktop software or Thermo Fisher
™
Connect
to view the flags and troubleshooting details for
failed wells.
If the verification fails again, order a replacement
plate. If the replacement plate fails, contact
Support.
Chapter 5
Calibrate and verify instrument performance
Perform instrument verification using RNase P plates
5
64
QuantStudio
™
1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide
