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TDX-OLI-DMR-PS-0619 Rev. 02

User Manual

Micro SSP™ Gel System

Catalog #: MGS108

Содержание MGS108

Страница 1: ...TDX OLI DMR PS 0619 Rev 02 User Manual Micro SSP Gel System Catalog MGS108...

Страница 2: ...Hannover Germany 2017 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified Specifications terms...

Страница 3: ...Symbols 3 Micro SSP Gel System Preparation 5 Electrophoresis Procedure 8 Cleaning and Safety Precautions 11 Cleaning 11 Gel Box Cleaning Precautions 11 Power Rating 11 Electrical Safety Precautions 12...

Страница 4: ...onal lane total of 108 sample wells Same footprint as a standard 96 well microtiter plate Low reagent volume requirement Approximately 3 minute run time A leveling bubble and adjustable leveling knobs...

Страница 5: ...Description Catalog 1 Gel Box MGS XGB9 1 Gel Comb Holder MGSCH9 1 Gel Box Cover MGS XC Symbols CAUTION Refer to safety information in accompanying documents If the Micro SSP Gel System equipment is u...

Страница 6: ...SP Gel System User Manual TDX OLI DMR PS 0619 Rev 02 4 Class II Equipment Direct Current DC Voltage Manufacturer Serial Number Read Usage Instructions Waste Electrical and Electronic Equipment WEEE Im...

Страница 7: ...m for any cracks or damage that may have occurred during transport 2 Pour water into the Gel Box as you would with buffer and look for any evidence of leakage Empty and dry the Gel Box CAUTION Discard...

Страница 8: ...he leveling bubble this assures a level surface figure 4 The adjustment thumbscrews should not be disturbed after it has been leveled Figure 4 Leveling bubble 7 Orient and fully insert all 14 Gel Comb...

Страница 9: ...an pour a gel with an even thickness over the entire surface of the Gel Box Ensure that the agarose covers the entire surface evenly This may be done by tilting the Gel Box and Base back and forth to...

Страница 10: ...ading Important Amplified DNA samples should be loaded immediately after buffer is poured Ensure all wells are filled and the gel is evenly covered with buffer The Gel System should be used by trained...

Страница 11: ...rn on the power supply and set the voltage according to kit product insert Turn off the power supply 4 While aligning the color corded sides place Cover onto the Gel box such that the bottom of the Co...

Страница 12: ...ON Be attentive of any evidence of leakage from the system If any leakage is detected immediately turn off the power supply Do not move Gel System while running test 8 Turn off the power supply loosen...

Страница 13: ...ce of the product Dry with a clean damp cloth or towel To clean the Cover lightly dust do not rub the surfaces with a soft clean cloth Then carefully wipe the surfaces with a soft wet cotton terry clo...

Страница 14: ...Gel System If any leakage is detected immediately turn off Power Supply Once finished remove the Cover turn off power supply return voltage setting to zero and disconnect leads from the power supply...

Страница 15: ...For service requests contact Technical Support Only to be supplied by One Lambda A Thermo Fisher Scientific Brand Components must be disposed of in accordance with WEEE Directive Description Cat No Ge...

Страница 16: ...gel and buffer levels The safety switch in the Cover is not engaged Place the Cover down firmly by pressing it down until a click can be heard this turns on the safety switch Check for proper operati...

Страница 17: ...Prepare fresh batch of reagent and protect it from light Prepare only enough fresh agarose solution for 1 2 days worth of work Not enough fluorescence Wear appropriate PPE when performing UV Photogra...

Страница 18: ...ke sure each sample well and electrode well is filled with running buffer before loading samples Not enough samples loaded Load all PCR samples to each well Sample lost during loading A mechanical pip...

Страница 19: ...trophoresis after the dye has migrated 0 5 cm into the gel UV light bulb in the trans illuminator is out of order or weak Check and if necessary replace UV light bulb s according to the manufacturer s...

Страница 20: ...ng and other breakage Replace as required Undissolved agarose Make sure agarose is completely melted Gel ran too fast Do not exceed recommended voltage Evaporation of running buffer Use a gel as soon...

Страница 21: ...e gel Difficult to visualize bands Incorrect background color Use a gray or black background Excessive leaking of samples when a multi channel pipette is used Leaking tips or tips not aligned Make sur...