ThermoFisher Scientific invitrogen iSort Series, Руководство пользователя

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68 iSort
™
Automated Cell Sorter User Guide
Drop Delay Calibration
Overview The drop delay value represents the distance between the point of interrogation of
the sample and the exact point where the sample stream breaks off into droplets that
contain the particle of interest. Therefore, the stability of the break-off dictates the
accuracy of the sorting.
Under typical operation, the iSort
™
Automated Cell Sorter automatically calibrates
the optimal drop delay value during system preparation before an Analysis or Sort
(page 34). To do this, the instrument checks flow stability and drop formation, and
determines charge timing automatically without any user input.
The Drop Delay Calibration function allows authorized users and service personnel
to troubleshoot and fine tune the drop delay value.
IMPORTANT!
The Drop Delay Calibration function is only available to authorized
users and service personnel for troubleshooting and verification purposes. Do not
run the Drop Delay Calibration unless you are authorized to do so.
Required materials •
Attune ™ Performance Tracking Beads
•
Microscope slide
•
Fluorescent microscope
•
10X PBS, flow cytometry grade
•
Sheath fluid (1X PBS, flow cytometry grade)
•
Deionized water
Run Drop Delay
Calibration
1. On the Calibration & Performance Test screen, press Drop Delay Calibration.