ThermoFisher Scientific Invitrogen iBright CL1000, Руководство пользователя, Страница: 13 / 48

Fluorescent Blots (FL1000 model only)
Mode to image fluorescent Western blots using a single channel or up to 4 channels
using excitation wavelengths of approximately 455-485 nm, 515-545 nm, 610-635 nm,
655-680 nm, and 745-765 nm (not available on CL1000).
1.
Touch Choose a dye to select one dye or multiple dyes from the list. See “Select a
dye in the Fluorescent Blots mode“ on page 13 for selection details.
2.
Touch Done.
3.
Touch Smart Exposure. Alternatively, for manual exposure, touch More
options
4
Manual Exposure.
The imager will acquire a series of short exposures for each dye type selected. It
will then render preview images and a recommended exposure time for each
channel. These are not real images.
4. To adjust or set a manual exposure time, select a method:
• Touch or within dial.
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
Note: To adjust or set the exposure time for a specific dye channel, see “Edit dye
channel exposure time“ on page 14.
5.
Touch Enter.
The image preview will update in real-time.
6.
Touch Capture to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
7. If an acceptable image, touch Export, Gallery or Analyze. If an unacceptable
image, touch More options
4
Image adjust to optimize image or touch Trash to
remove image, then return to steps 3-5 to adjust exposure conditions.
If you are capturing an image in the Fluorescent Blots mode (FL1000 model only),
select up to 4 dyes before imaging.
1. Touch a dye channel.
2. Select a dye from the list.
3. (Optional) Select a false color from the list and enter an exposure time.
4. Touch Done.
Repeat as many times as necessary, up to 4 dyes.
Image using
Fluorescent Blots
(FL1000 model
only)
Select a dye in the
Fluorescent Blots
mode
Operate the iBright
™
imager
Fluorescent Blots (FL1000 model only)
iBright
™
Imaging Systems User Guide 13