.
1 July 2019
-3-
E-Base
™
DNA electrophoresis protocol
Step
Action
12–30 min
4
Run the gel
a. Select the EG program (default run time 12 minutes) for running E-Gel
™
cassettes by
pressing and releasing the “pwr/prg”.
b. Select the recommended run time for a specific gel type by pressing and releasing the time
button, then press and hold the time button to increase the time. Release time button when
the desired run time for the gel is reached.
Gel type
Recommended run time
Maximum run time
E-Gel
™
48
agarose gel
20 min
25 min
E-Gel
™
96
agarose gel
12 min
17 min
c. Start the run by pressing and releasing the “pwr/prg” button. The red indicator light will
change to green.
5
End the run
a. A flashing red indicator light and rapid beeping indicates the end of the run. Press and
release “pwr/prg” to stop the device.
b. For better detection sensitivity, allow the gel to cool down for 10 minutes after the end of
the run.
1–2 min
6
Analyze the gel
a. Visualize the with a DNA imager using blue-light transillumination (e.g., with the E-Gel
™
Imager System with Blue Light Base).
·
SYBR Safe
™
DNA gel stain has an excitation maxima at 280 and 502 nm, and an emission
maximum at 530 nm when bound to nucleic acid.
·
Use the E-Editor
™
2.0 software available at
to analyze 96-well
format digital images.
