8
•
Troubleshooting
UHPLC System
Operating Manual
Page 123
Possible Cause
Remedial Action
UV/Vis lamp defective
or not properly
installed
Check that the lamp is correctly installed. Replace the lamp (refer to
Detector
Operating Manual
).
Piston calibration value
mismatch
Verify that the calibration value of the piston (imprinted on the rear of the piston)
corresponds to the related piston calibration value in the Chromeleon software.
Adapt the value in the Chromeleon software if necessary.
8.2.8
Temperature Control
Column compartment temperature does not change although the set point has not been
reached
Possible Cause
Remedial Action
Ambient temperature
too high or setpoint
lower than the
specified difference to
the ambient
temperature
Reduce the ambient temperature (for example, by ventilating the room).
Temperature control
turned off
Check the setting for TempCtrl in the Chromeleon software (
TempCtrl=On
) turns
temperature control on).
Ventilation grid
obstructed
Make sure that the ventilation grids of the column compartment are not obstructed
in any way.
Ambient air enters
column chamber
•
Improper sealing of the column chamber may impair the heating and cooling
performance of the column compartment and lead to a considerable amount of
condensed water.
•
When guiding capillaries through the column chamber cover seal, make sure that
the cover seals properly. If the chamber cover seal is damaged, replace the
chamber cover (refer to
Column Compartment Operating Manual
).
•
Also, make sure that the front door is properly closed.
Column compartment
defective
Contact Technical Support.
Pre-heater does not reach set temperature
Possible Cause
Remedial Action
Pre-heater not
installed correctly
The performance may be impaired if the thermal contact is imperfect, or if the pre-
heater inlet and outlet are exchanged. Reinstall the pre-heater (refer to
Column
Compartment Operating Manual
).
Pre-heater defective
Replace the pre-heater (refer to
Column Compartment Operating Manual
).
Содержание thermoscientific Vanquish UHPLC System
Страница 1: ...thermoscientific Vanquish UHPLC System Operating Manual 4820 3601 EN Revision 4 0 December 2019 ...
Страница 4: ...Contacting Us Page 4 UHPLC System Operating Manual ...
Страница 28: ...2 Safety Page 28 UHPLC System Operating Manual ...
Страница 34: ...3 System Overview Page 34 UHPLC System Operating Manual ...
Страница 38: ...4 Unpacking Page 38 UHPLC System Operating Manual ...
Страница 82: ...5 Installation Page 82 UHPLC System Operating Manual ...
Страница 100: ...6 Operation Page 100 UHPLC System Operating Manual ...
Страница 112: ...7 Maintenance and Service Page 112 UHPLC System Operating Manual ...
Страница 128: ...8 Troubleshooting Page 128 UHPLC System Operating Manual ...
Страница 132: ...9 Specifications Page 132 UHPLC System Operating Manual ...
Страница 144: ...11 Appendix Page 144 UHPLC System Operating Manual ...
Страница 148: ...Thermo Fisher Scientific Inc 168 Third Avenue Waltham Massachusetts 02451 USA www thermofisher com ...