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T3/G3 5-3 Thermo Fisher Scientific
Chapter 5
Hazard, Precautions and Limitations of Use
Thermo recommends the use of sealed buckets, which are certified for
bio-containment through worldwide recognized testing procedures
performed in recognized external laboratories.
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Aerosols are produced naturally when using uncapped tubes or bottles.
They are also produced when a container deforms or breaks provoking
a small or large sample leak.
We draw your attention to the use of tubes in angle rotors. Even if the
liquid level does not touch the cap when in a static state, it could do so
during centrifugation and leak. You should therefore follow the
instructions of the tube supplier concerning the fill level and sealing of
the tube.
Once a biological risk is known or suspected, samples should be placed
in a sealed container. Should such containers not be available, sealed
accessories should be used, such as angle rotors with sealed lids.
When aerosols are created in a centrifuge bowl, they persist in the air
for a period of 3-5 minutes after the rotor stops moving. Indeed, the
action of opening the lid itself causes the dispersion of aerosols into the
environment.
When an elevated risk of aerosols or of breakage is perceived the
accessories should be handled using gloves and opened in an
environment ensuring the protection of the operator (safety cabinet,
glove chamber, wearing a mask ...), even if they are apparently
sealed.
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Duration of aerosol presence
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