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Thermo Scientific Orion 320, 350 and 370 PerpHecT

LogR

Meter User Guide

1-1

Chapter 1

Introduction

Thermo Scientific Orion pH and ISE meters are designed for every

application, from basic pH measurements to advanced concentration analysis

using ion selective electrodes . This user guide contains information on the

preparation, operation and maintenance for the 320, 350 and 370 PerpHecT

LogR meters . The 320 meter measures pH, millivolts (mV) and temperature .

The 350 meter measures pH, millivolts (mV), oxidation-reduction potential

(ORP) and temperature . The 370 meter measures pH, concentration (ISE),

millivolts (mV), oxidation-reduction potential (ORP) and temperature .

All PerpHecT meters feature patented digital LogR technology, which allows

fully temperature compensated measurements without a separate ATC probe .

For maximum system accuracy, use Thermo Scientific Orion PerpHecT

and PerpHecT ROSS pH electrodes . PerpHecT meters are microprocessor-

controlled and feature pH autocalibration, patented digital LogR pH

measurement mode, sealed keypads and large easy-to-read LED displays .

PerpHecT meters are ideal for busy, multi-user laboratories or plant

environments . The pH measurements are easy with the help of advanced

features such as autocalibration, LogR mode, automatic temperature

compensation, choice of pH resolution and diagnostic operator assistance

codes .

• The 320 PerpHecT meter is a basic meter designed for routine pH and

mV measurements .

• The 350 PerpHecT meter offers an RS232 interface for connecting the

meter to a printer or a computer . Other features include GLPcheck for

monitoring electrode performance and GLPdoc that creates printouts

that are compatible with good laboratory practice (GLP) requirements .

• The 370 PerpHecT meter offers basic ISE functions for measuring ion

concentrations using an ion selective electrode and an RS232 interface for

connecting the meter to a printer or a computer . Other features include

GLPcheck for monitoring electrode performance and GLPdoc that

creates printouts that are compatible with good laboratory practice (GLP)

requirements .

General Information

Содержание Orion 320

Страница 1: ...Thermo Scientific Orion 320 350 and 370 PerpHecT LogR Meter User Guide ...

Страница 2: ...solution SAOB SMART AVERAGING Smart CheK SMART STABILITY Stacked Star Navigator 21 Stat Face The Enhanced Lab ThermaSense Triode TRIUMpH Unbreakable pH Universal Access are trademarks of Thermo Fisher Guaranteed Success and The Technical Edge are service marks of Thermo Fisher PerpHecT meters are protected by U S patent 6 168 707 PerpHecT ROSS are protected by U S patent 6 168 707 ORION Series A m...

Страница 3: ...on 3 6 LogR Calibration 3 7 mV ORP Measurements 3 8 Automatic Temperature Compensation 3 8 Manual Temperature Compensation 3 8 Operating the 350 Meter 4 1 Meter Power Up 4 1 Setup Menu 4 1 pH Calibration 4 3 Three Point Autocalibration 4 4 Two Point Autocalibration 4 5 One Point Autocalibration 4 6 Manual Calibration 4 7 LogR Temperature Calibration 4 8 LogR Calibration 4 9 mV ORP Measurements 4 1...

Страница 4: ...tion 5 12 GLPcheck Electrode Diagnostic Procedure 5 13 Interfacing the 350 and 370 Meters with Printers and Computers 6 1 Customer Services 7 1 Troubleshooting Guide 7 1 Meter Self Test and Checkout Procedures 7 1 Meter Self Test 7 2 Meter Self Test Operator Assistance Codes 7 3 320 Meter Check Out 7 3 350 Meter Check Out 7 4 370 Meter Check Out 7 5 Operator Assistance Codes 7 6 Assistance 7 8 War...

Страница 5: ... and feature pH autocalibration patented digital LogR pH measurement mode sealed keypads and large easy to read LED displays PerpHecT meters are ideal for busy multi user laboratories or plant environments The pH measurements are easy with the help of advanced features such as autocalibration LogR mode automatic temperature compensation choice of pH resolution and diagnostic operator assistance co...

Страница 6: ...1 2 Thermo Scientific Orion 320 350 and 370 PerpHecT LogR Meter User Guide Figure 1 370 PerpHecT LogR Meter ...

Страница 7: ...ain middle field Temperature is displayed in the upper field The lower display shows millivolts The current operation mode is indicated along the right side of the display The buffers used during the last pH calibration are designated by the indicators along the left side of the display See Figure 2 Figure 2 370 PerpHecT Meter Display Meter Display ...

Страница 8: ...th an ATC probe The LogR icon is lit when LogR temperature measurement is activated If neither is on manual temperature setting is active ready Displayed when the electrode signal has stabilized pH conc rel mV mV and C and when the calibration standard input has stabilized The 320 meter has a six button keypad that is used to control all meter functions Key Function mode Press to select the measur...

Страница 9: ...n points May also be used to scroll through the setup menu without changing any parameters scroll Press to change a setup parameter Also used in the measurement mode to scroll manual temperature settings print Press to print measurement data cal Press to initiate pH rel mV or ISE 370 only calibration setup Press to enter setup menu Setup menu is entered to change meter operating parameters and to ...

Страница 10: ...nd then push in and turn the connector clockwise to lock it into position Electrodes with U S standard connectors may be used with an adaptor Cat No 090033 Connect reference electrodes with pin tip connectors by pushing the connector directly into reference input See Figure 4 ATC Probe Attach an ATC probe to the ATC input on the back of the meter by sliding the connector straight in until it is fi...

Страница 11: ...adings of the electrode to pH values changes with temperature At 25 C a 10 degree change in temperature changes the slope by 3 4 How important is direct temperature compensation to pH measurement To see the effects of temperature look up your approximate sample temperature on the left side of Table 1 Read across the top to your approximate pH value The value shown at the intersection is the error ...

Страница 12: ...ing the electrical resistance of the glass sensing bulb as the temperature probe The logarithm of the resistance of the bulb varies almost linearly with the reciprocal of the absolute temperature see Figure 5 Almost all common pH glasses show a similar decrease in resistance with increasing temperature and can be used with other meters However only the exact shape of the curve of PerpHecT electrod...

Страница 13: ...he value is displayed as a percent of theoretical slope The default setting is 100 This function is for display purposes only The value cannot be changed in the setup menu To change the slope value perform a two point calibration or set the slope during a one point calibration Press the yes key to advance to next menu function LogR When the LogR function is accessed the LogR icon will be shown whi...

Страница 14: ...se of a separate ATC probe The PerpHecT meters measure the resistance of the pH sensing glass and converts the value to temperature compensated pH measurements For optimum results PerpHecT electrodes or PerpHecT ROSS electrodes should be used For the best results it is recommended that the meter be calibrated for temperature in the LogR mode Alternately an ATC probe may be used If LogR or an ATC p...

Страница 15: ...ey to accept the buffer value 5 The pH 4 or pH 10 icon will be displayed Remove the electrode from pH 7 00 buffer and rinse it with distilled water Place the electrode in either pH 4 01 or 10 01 buffer depending on calibration range that was selected When the ready icon is shown press the yes key to accept the buffer value 6 SLP will be displayed while the calculated slope is displayed 7 The meter...

Страница 16: ...cating electrode stability press the yes key 5 SLP will be displayed while 100 0 or the previous calibration slope value is displayed This value can be edited using the scroll keys Press the yes key to save the slope value 6 The meter will automatically proceed to the measurement mode The ready icon will be shown to indicate electrode stability 7 If operating in the LogR mode or using an ATC probe...

Страница 17: ... 3 Press the cal key The display will show CAL for two seconds The last calibration buffer range will be displayed Use the scroll keys to select the SET option Press the yes key to accept the SET option 4 P1 will be displayed Place the electrode into the first calibration buffer When the ready icon is displayed the user defined buffer value will be shown Press the yes key to accept the value 5 P2 ...

Страница 18: ... 10 C and 30 C For temperature span greater than 20 C a three point temperature calibration is recommended For many samples a single point calibration near the expected sample temperature will yield adequate results A constant temperature bath or a hot plate is recommended to hold the temperature of calibrating solution For best results during temperature calibration it is recommended that a pH bu...

Страница 19: ...yes key 3 The display will show CAL 1 in the main readout The measured temperature value based on the last calibration will be displayed Place the PerpHecT or ROSS PerpHecT pH electrode into a temperature controlled pH buffer or tap water solution Measure the temperature of the solution using a NIST traceable thermometer When the LogR signal stabilizes the ready icon will be shown 4 Adjust the tem...

Страница 20: ...er See Figure 4 The 320 350 and 370 PerpHecT meters can be used with the PerpHecT epoxy body ATC probe Cat No 927005 the PerpHecT glass body ATC probe Cat No 927006 and the PerpHecT stainless steel body ATC probe Cat No 927007 For manual temperature compensation disconnect the ATC probe and disable the LogR function in the setup menu Manual temperature may only be set when the LogR function is dis...

Страница 21: ...tion To exit the setup menu press the mode key Slope The current pH electrode slope in the meter memory will be displayed The value is displayed as a percent of theoretical slope The default setting is 100 This function is for display purposes only The value cannot be changed in the setup menu To change the slope value perform a two point calibration or set the slope during a one point calibration...

Страница 22: ...ed in the main field Use the scroll keys to set the month Press the yes key to accept the month setting DAY will be displayed in the lower field with the current day in the main field Use the scroll keys to set the day Press yes to accept the day setting HOUR will be displayed in the lower field with the current hour displayed in the main field Use the scroll keys to set the hour Press the yes key...

Страница 23: ...ion curve is displayed Use of this scheme increases accuracy in the different regions of the calibration curve However the electrode slope may appear lower than normal especially if buffers from the pH extremes pH less than 2 00 or pH greater than 12 00 are used When operating in the LogR mode the meter does not require the use of a separate ATC probe The PerpHecT meters measure the resistance of ...

Страница 24: ...icon is shown indicating electrode stability press the yes key to accept the buffer value 6 The 10 buffer icon will be displayed Place the electrode in the pH 10 01 buffer The reading will be displayed and updated as the calibration continues When the ready icon is shown indicating electrode stability press the yes key to accept the buffer value 7 SLP will be displayed while the calculated slope i...

Страница 25: ...ey to accept the buffer value 5 The pH 4 or pH 10 icon will be displayed Remove the electrode from pH 7 00 buffer and rinse it with distilled water Place the electrode in either pH 4 01 or 10 01 buffer depending on calibration range that was selected When the ready icon is shown press the yes key to accept the buffer value 6 SLP will be displayed while the calculated slope is displayed 7 The meter...

Страница 26: ...cating electrode stability press the yes key 5 SLP will be displayed while 100 0 or the previous calibration slope value is displayed This value can be edited using the scroll keys Press the yes key to save the slope value 6 The meter will automatically proceed to the measurement mode The ready icon will be shown to indicate electrode stability 7 If operating in the LogR mode or using an ATC probe...

Страница 27: ... Press the cal key The display will show CAL for two seconds The last calibration buffer range will be displayed Use the scroll keys to select the SET option Press the yes key to accept the SET option 4 P1 will be displayed Place the electrode into the first calibration buffer When the ready icon is displayed the user defined buffer value will be shown Press the yes key to accept the value 5 P2 wi...

Страница 28: ... 10 C and 30 C For temperature span greater than 20 C a three point temperature calibration is recommended For many samples a single point calibration near the expected sample temperature will yield adequate results A constant temperature bath or a hot plate is recommended to hold the temperature of calibrating solution For best results during temperature calibration it is recommended that a pH bu...

Страница 29: ...yes key 3 The display will show CAL 1 in the main readout The measured temperature value based on the last calibration will be displayed Place the PerpHecT or ROSS PerpHecT pH electrode into a temperature controlled pH buffer or tap water solution Measure the temperature of the solution using a NIST traceable thermometer When the LogR signal stabilizes the ready icon will be shown 4 Adjust the tem...

Страница 30: ...efined value if necessary Press the yes key to accept the calibration 5 Place the electrode in a sample When the reading stabilizes record the relative mV and temperature values For automatic temperature compensation connect an ATC probe to the meter and disable the LogR function in the setup menu Attach the ATC probe to the 3 5 mm phone jack on the rear panel of the meter See Figure 4 The 320 350...

Страница 31: ...rs Refer to the Three Point Autocalibration procedure 3 Upon successful completion of the three point calibration sequence gLP CAL g00d will appear on the meter display and the meter will automatically send the documented results to the external device 4 The time date meter serial number if entered slope average Eo mV and temperature of each buffer and Electrode Good will be printed 5 Unsuccessful...

Страница 32: ...4 12 Thermo Scientific Orion 320 350 and 370 PerpHecT LogR Meter User Guide Notes ...

Страница 33: ...ope The current electrode slope in the meter memory will be displayed In pH mode the value is displayed as a percent of theoretical slope The default setting is 100 In the ISE mode the value is displayed in mV decade The default setting is 59 2 mV decade This function is for display purposes only The value cannot be changed in the setup menu To change the slope value perform a two point calibratio...

Страница 34: ...ed in the main field Use the scroll keys to set the month Press the yes key to accept the month setting DAY will be displayed in the lower field with the current day in the main field Use the scroll keys to set the day Press yes to accept the day setting HOUR will be displayed in the lower field with the current hour displayed in the main field Use the scroll keys to set the hour Press the yes key...

Страница 35: ...ion curve is displayed Use of this scheme increases accuracy in the different regions of the calibration curve However the electrode slope may appear lower than normal especially if buffers from the pH extremes pH less than 2 00 or pH greater than 12 00 are used When operating in the LogR mode the meter does not require the use of a separate ATC probe The PerpHecT meters measure the resistance of ...

Страница 36: ...icon is shown indicating electrode stability press the yes key to accept the buffer value 6 The 10 buffer icon will be displayed Place the electrode in the pH 10 01 buffer The reading will be displayed and updated as the calibration continues When the ready icon is shown indicating electrode stability press the yes key to accept the buffer value 7 SLP will be displayed while the calculated slope i...

Страница 37: ...ey to accept the buffer value 5 The pH 4 or pH 10 icon will be displayed Remove the electrode from pH 7 00 buffer and rinse it with distilled water Place the electrode in either pH 4 01 or 10 01 buffer depending on calibration range that was selected When the ready icon is shown press the yes key to accept the buffer value 6 SLP will be displayed while the calculated slope is displayed 7 The meter...

Страница 38: ...cating electrode stability press the yes key 5 SLP will be displayed while 100 0 or the previous calibration slope value is displayed This value can be edited using the scroll keys Press the yes key to save the slope value 6 The meter will automatically proceed to the measurement mode The ready icon will be shown to indicate electrode stability 7 If operating in the LogR mode or using an ATC probe...

Страница 39: ... Press the cal key The display will show CAL for two seconds The last calibration buffer range will be displayed Use the scroll keys to select the SET option Press the yes key to accept the SET option 4 P1 will be displayed Place the electrode into the first calibration buffer When the ready icon is displayed the user defined buffer value will be shown Press the yes key to accept the value 5 P2 wi...

Страница 40: ... 10 C and 30 C For temperature span greater than 20 C a three point temperature calibration is recommended For many samples a single point calibration near the expected sample temperature will yield adequate results A constant temperature bath or a hot plate is recommended to hold the temperature of calibrating solution For best results during temperature calibration it is recommended that a pH bu...

Страница 41: ...yes key 3 The display will show CAL 1 in the main readout The measured temperature value based on the last calibration will be displayed Place the PerpHecT or ROSS PerpHecT pH electrode into a temperature controlled pH buffer or tap water solution Measure the temperature of the solution using a NIST traceable thermometer When the LogR signal stabilizes the ready icon will be shown 4 Adjust the tem...

Страница 42: ...n electrodes Standards should bracket the sample range and be in the same concentration units The electrode slope displayed after the calibration is the slope between the two points used for the calibration curve Any convenient concentration units can be used including molarity M ppm percent etc The meter will not display the units so the operator must keep the type of concentration units consiste...

Страница 43: ...hown in the upper display field When the ready icon is shown indicating electrode stability use the scroll keys to set the value of the first standard Press the yes key to accept the standard one value 6 Remove the electrode s from the first standard rinse the electrode s with distilled water and place the electrode s in the most concentrated standard 7 P2 will be shown in the upper display field ...

Страница 44: ...efined value if necessary Press the yes key to accept the calibration 5 Place the electrode in a sample When the reading stabilizes record the relative mV and temperature values For automatic temperature compensation connect an ATC probe to the meter and disable the LogR function in the setup menu Attach the ATC probe to the 3 5 mm phone jack on the rear panel of the meter See Figure 4 The 320 350...

Страница 45: ...rs Refer to the Three Point Autocalibration procedure 3 Upon successful completion of the three point calibration sequence gLP CAL g00d will appear on the meter display and the meter will automatically send the documented results to the external device 4 The time date meter serial number if entered slope average Eo mV and temperature of each buffer and Electrode Good will be printed 5 Unsuccessful...

Страница 46: ...5 14 Thermo Scientific Orion 320 350 and 370 PerpHecT LogR Meter User Guide Notes ...

Страница 47: ...e printer are factory configured for the PerpHecT meters A computer interface cable Cat No 0ACBL0 is available for connecting the PerpHecT meters to the serial port of an IBM or compatible PC For other computers and communication devices a suitable cable must be fabricated The following technical information outlines the pin configurations data transfer hardware and software settings for the 350 a...

Страница 48: ...6 2 Thermo Scientific Orion 320 350 and 370 PerpHecT LogR Meter User Guide Figure 6 Printer Wiring Diagram ...

Страница 49: ...ecked The meter displays the number of the test in the main field Additional meter displays occur depending on the test being performed If any problems are found an operator assistance code corresponding to the test that failed will be displayed Note the code and press the yes key to continue If an operator assistance code appears during self test check to make sure that the shorting cap is secure...

Страница 50: ...e 5 If any problems are found during the self test refer to the meter self test operator assistance codes Test Function Display Test 1 Segment display checks meter display Ensures that all segments are lit Test 2 RAM check verifies the proper operation of the Random Access Memory Test 3 External input checks the electrode input channel Electrode must be disconnected and BNC shorting cap must be pl...

Страница 51: ...n prompted during the self test 1 After the self test is complete the meter will return to the measurement mode Keep all of the electrodes disconnected from the meter and keep the BNC shorting cap attached to the BNC input on the meter Press the mode key until the pH mode indicator is displayed The main display should read a steady 7 00 0 02 a If the display does not read a steady 7 00 0 02 press ...

Страница 52: ...alue is displayed Set this value to 100 0 using the scroll keys Press the yes key to save the slope value d The meter should advance to the pH measurement mode and the display should read a steady 7 00 0 02 2 In the measurement mode press the mode key until the meter displays the rel mV icon The display should read 0 0 0 2 rel mV a If the display does not read 0 0 0 2 rel mV press the cal key The ...

Страница 53: ...alue is displayed Set this value to 100 0 using the scroll keys Press the yes key to save the slope value d The meter should advance to the pH measurement mode and the display should read a steady 7 00 0 02 2 In the measurement mode press the mode key until the meter displays the rel mV icon The display should read 0 0 0 2 rel mV a If the display does not read 0 0 0 2 rel mV press the cal key The ...

Страница 54: ...s Refer to the electrode user guide for instructions on how to clean and condition the electrode E 21 Auto Buffer Recognition ABR Error pH Autocalibration Error The electrode voltage being measured is greater than 0 5 pH units from the nominal value for the pH buffer Verifythat pH 4 01 7 00 and 10 01 buffers are being used Recalibrate the meter system using fresh buffers Refer to the electrode use...

Страница 55: ...s from each other E 35 Bad LogR Standard The error code will occur when two calibration standards do not differ in temperature by the minimum allowable difference in LogR mode The difference between temperature calibration points must be at least 5 C The difference in the entered value must be greater than 5 C E 36 No LogR Cal The error code will occur when the calibration coefficients from a LogR...

Страница 56: ... Class A computing device pursuant to Subpart J of Part 15 of FCC Rules which are designed to provide reasonable protection against such interference in a commercial environment Operation of the meter in a residential area may cause interference in which case the user at his own expense will be required to take whatever measures may be required to correct the interference Note To meet or exceed FC...

Страница 57: ...51 Laboratory Equipment Emissions EN 55022 Emissions FCC Part 15 Class A Immunity EN50082 1 Generic Immunity IEC 801 2 ESD Susceptibility IEC 801 3 Radiated Susceptibility IEC 801 4 Conducted Susceptibility These Thermo Scientific Orion products have been manufactured in compliance with the provisions of the relevant Thermo Fisher Scientific manufacturing and test documents and processes Further t...

Страница 58: ...7 10 Thermo Scientific Orion 320 350 and 370 PerpHecT LogR Meter User Guide Notes ...

Страница 59: ...r 240 V 035000 350 PerpHecT meter 110 V and 9272BN PerpHecT Sure Flow Ag AgCl pH electrode 035001 350 PerpHecT meter 220 V and 9272BN PerpHecT Sure Flow Ag AgCl pH electrode 0350A0 350 PerpHecT meter 110 V and 8272BN PerpHecT ROSS Sure Flow pH electrode 0350A1 350 PerpHecT meter 220 V and 8272BN PerpHecT ROSS Sure Flow pH electrode 0350G0 350 PerpHecT meter 110 V 8272BN PerpHecT ROSS Sure Flow pH ...

Страница 60: ...110 V 9156APWP AquaPro pH electrode and swing arm electrode stand 0320A9 320 PerpHecT meter 110 V 0320A8 320 PerpHecT meter 220 V 0320A7 320 PerpHecT meter 240 V 090043 Swing arm electrode stand 090070 Basic electrode stand and holder 090045 BNC shorting cap PRT300 Ink printer 110 V and interface cable PRT301 Ink printer 220 V and interface cable PRT302 Ink ribbon for PRT300 and PRT301 printers 0A...

Страница 61: ...ution 19 L 5 gallon cubitainer 810001 ROSS pH electrode storage solution optimizes ROSS pH electrode performance and life 475 mL 911110 pH electrode rinse solution 10 PerpHecT individual buffer pouches 911125 pH electrode rinse solution 25 PerpHecT individual buffer pouches 900020 pH electrode cleaning solution kit includes 1 x 30 mL bottle each of cleaning solution A and C 1 x 60 mL bottle each o...

Страница 62: ...cy N A 0 2 or 0 05 of reading whichever is greater 0 2 or 0 05 of reading whichever is greater Temperature Range 0 0 100 C 0 0 100 C 0 0 100 C Temperature Resolution 0 1 0 1 0 1 Temperature Accuracy 1 0 1 0 1 0 Temperature Compensation Automatic or Manual Automatic or Manual Automatic or Manual LogR Temp Accuracy 20 C Calibration Range 0 5 C 0 5 C 0 5 C Concentration Range N A N A 0 000 19900 Conc...

Страница 63: ... Error above 20 C C Average Temp Compensation Error 0 to 25 C pH units Average Temp Compensation Error above 20 C pH units 8202BN 0 36 0 10 0 004 0 001 8203BN 0 13 0 02 0 002 0 000 8235BN 0 04 0 14 0 000 0 002 8256BN 0 22 0 06 0 003 0 001 8272BN 0 23 0 06 0 003 0 001 9202BN 0 21 0 06 0 003 0 001 9203BN 0 20 0 10 0 002 0 001 9206BN 0 29 0 06 0 003 0 001 9207BN 0 16 0 07 0 002 0 001 9256BN 0 27 0 07...

Страница 64: ...verage Temp Compensation Error 0 to 25 C pH units Average Temp Compensation Error above 20 C pH units 8202BN 0 04 0 03 0 000 0 000 8203BN 0 04 0 01 0 000 0 000 8235BN 0 07 0 06 0 001 0 001 8256BN 0 04 0 06 0 000 0 001 8272BN 0 02 0 01 0 000 0 000 9202BN 0 03 0 06 0 000 0 001 9203BN 0 06 0 05 0 001 0 001 9206BN 0 02 0 02 0 000 0 000 9207BN 0 07 0 07 0 001 0 001 9256BN 0 02 0 00 0 001 0 000 9272BN 0...

Страница 65: ...A 3 Thermo Scientific Orion 320 350 and 370 PerpHecT LogR Meter User Guide Notes ...

Страница 66: ...A 4 Thermo Scientific Orion 320 350 and 370 PerpHecT LogR Meter User Guide Notes ...

Страница 67: ...tific Environmental Instruments Water Analysis Instruments 166 Cummings Center Beverly MA 01915 USA Tel 978 232 6000 Toll Free 800 225 1480 Dom Fax 978 232 6015 Int l Fax 978 232 6031 www thermo com water 255007 001 Rev A ...

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