Thermo Scientific Invitrogen Countess II FL, Руководство пользователя

Страница: 20 / 82

1. Before loading your sample into the Countess ™ II FL Reusable Slide, place a
cover slip on the counting chamber, making sure the cover slip is clean and free
of grease.
2. Gently pipet 10 µL of the sample into the sample inlet, allowing capillary action
to draw the sample into the counting chamber. A properly loaded counting
chamber should have a thin, even film of fluid under the cover slip.
3.
After using the Countess ™ II FL Reusable Slide, rinse the glass slide and cover
slip with water, and then clean with 70% ethanol. Use Kimwipes ™ laboratory
tissues to clean and dry the slides, as needed.
Note: Each chamber in the Countess ™ Cell Counting Chamber Slide or the
Countess ™ II FL Reusable Slide has a 10-µL sample capacity. Do not overfill the
slide chambers.
Slide operation
The Countess ™ II FL instrument accepts both disposable Countess ™ Cell Counting
Chamber Slides and glass Countess ™ II FL Reusable Slides on interchangeable, slide-
specific carriers.
1.
To use the plastic, disposable Countess
™
Cell Counting Chamber Slide with the
Countess
™
II FL Automated Cell Counter, insert the slide carrier (black, see
image) into the slide port of the instrument until it clicks into place.
Note: The Countess
™
II FL Automated Cell Counter is shipped with the
disposable slide carrier already installed
Load Countess ™ II
FL reusable slide
Countess
™
cell
counting chamber
slide
Chapter 3 Getting started
Slide operation
3
20 Countess
™
II FL automated cell counter User Guide