3
TIP EJECTION (Fig.3)
1. To remove the tip and plunger from the pipette simply
press the operating button all the way down.
2. Then release the operating button.
3. This action simutaneously ejects the tip and the
plunger.
PIPETTING TECHNIQUE
Figure 6
A = Ready Position
B = Pipetting Stop
C = Tip Ejection
Ensure that clean tips are firmly pushed on to the tip cones
of the pipette.
The operation of the pipette is controlled by the operating
button. For the best possible accuracy:
Operate the button slowly and with steady speed at all
times, particularly when working with liquids having high
viscosity. Never let the operating button snap back. When
working with serum or biological fluids prime the tips with
the liquid before pipetting. This is done by filling and
emptying the tips.
Hold the pipette vertically (10 degrees maximum from
vertical) during the liquid intake. For maximum hand
control, hold the pipette tightly in the hand with the
"Grippy" resting on the index finger.
Fill a clean reagent reservoir with the liquid to be
dispensed.
1. Depress the operating button to the pipetting stop.
2. Dip the tip just under the surface of the liquid in the
reservoir (2-3 mm) and slowly release the operating
button. Withdraw the tip from the liquid touching them
against the edge the reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the operating
button to the pipetting stop.
4. Release the operating button to the ready position.
If necessary, change the tips and continue with the
pipetting.
