6
Running an Xcalibur Experiment by Using ETD
Nth Order Double Play (ETD) Experiment
46
MS/ETD System Getting Started Guide
Thermo Scientific
8. (For Scan Event 2 and Scan Event 3 only) Click
Settings
to open the Data Dependent
Settings dialog box (
).
Figure 33.
Data Dependent Settings dialog box
9. In the left pane, under the Scan Event list, click
Activation
to see the activation
parameters (
).
The activation type is automatically set to ETD.
Figure 34.
Activation parameters
10. In the Default Charge State box, enter a value of
5
or more.
11. In the Isolation Width (
m/z
) box, enter a value between
2
and
3
.
The Normalized Collision Energy and Activation Q parameters are unavailable because
they are not applicable to ETD.
12. In the Activation Time (ms) box, leave the default value or enter a different value as
described in
“Optimizing the Reagent Ion Reaction Time”
.
