4
Daily Operation
Before Operating the LTQ XL/ETD or LTQ Velos/ETD System
26
MS/ETD System Getting Started Guide
Thermo Scientific
Checking the Vacuum Pressure
Check the vacuum pressure before operating the system.
To check the vacuum pressure
1. In the Tune Plus window, choose
Setup > Vacuum
).
Figure 16.
Ion Trap page
Note
When on, the System LED on the front panel of the MS detector turns green and the
high voltage to the electron multipliers turns on.
The following conditions can cause the MS/ETD System to remain in standby mode
even though you try to turn it on:
• Attempting to turn on the reagent ion source when the restrictor heater, source
heater, and transfer line heater are not at their target temperatures.
• When either the LTQ XL/ETD or LTQ Velos/ETD MS detector or the
MS/ETD System goes into standby mode. Reagent vial nitrogen cooling turns on
if the vials are at an elevated temperature.
• Exception: If you put the LTQ XL/ETD or LTQ Velos/ETD system in standby
mode, the cooling nitrogen turns on after a one hour delay.
• Whenever the pressure in the LTQ XL or LTQ Velos MS detector or the
MS/ETD System exceeds its protection limit. Reagent vial nitrogen cooling turns
on if the vials are at an elevated temperature.
• Whenever the flow of reagent ions becomes insufficient as determined by the
AGC setting. When this occurs the LTQ XL/ETD or LTQ Velos/ETD system
completes the Xcalibur sequence step in progress before going into standby mode.
This prevents the loss of analysis results that might be affected by the reduced
reagent ion flow.
