Thermo Scientific Applied Biosystems QuantStudio 6 Pro, Руководство пользователя

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Configure ramp rates
The ramp rate is how quickly the temperature changes from one stage or step to another stage or step.
The maximum ramp rate for the instrument is 6.5°C/second.
1.
In the Method tab, tap Edit
4
Advanced
4
Ramp rates .
Note: Use or to navigate through the method if it is not all displayed.
2.
Tap a ramp rate, then edit the value.
3.
Tap Done .
Add or remove a pause
A pause allows you to open the drawer to add additional reagents to the plate.
1.
In the Method tab, tap Edit
4
Advanced
4
Add pause .
Note: Use or to navigate through the method if it is not all displayed.
2.
Tap (Pause) .
3.
Enter a temperature and the cycle.
A pause in the thermal cycling protocol is denoted with a . The cycle of the pause and the
temperature of the pause are also denoted.
4.
Tap (Pause) to remove the pause.
5.
Tap Done .
Options to edit the well details of a plate file
The following files can be edited:
• A system template (see page 47 )
A system template that is edited must be saved as a plate file. A system template cannot be
overwritten.
• A saved plate file (see “Create and run a plate from a system template or a saved plate file”
on
page 47 )
• The plate file associated with the last run (see “Repeat the last run” on page 49 )
Chapter 4
Edit a plate before starting a run
Options to edit the well details of a plate file
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QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio ™ 7 Pro Real-Time PCR System User Guide