Thermo Scientific AMQAF2000, Руководство пользователя

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Cell count and cell viability assays
Count cells in brightfield
Capture and count
To bypass adjusting individual parameters before the brightfield count, you can select Rapid capture at
the top of the Home screen. This will automatically set the parameters and perform a count as soon as
the slide is inserted into the instrument. To adjust parameters prior to the count, follow the procedure
below.
1.
Load the samples into the instrument as described in Chapter 4, “Slide preparation and operation”.
2.
(Optional) : Press Templates and load the desired gating parameter settings as described in
“Load a template” on page 16 .
3.
Insert the sample slide into the slide port, making sure that the sample side is inserted completely
into the instrument. You will hear a soft click if the slide is pushed in correctly.
4.
When the slide is inserted, the instrument automatically illuminates the sample, sets the intensity of
brightfield illumination, and auto focuses on the cells.
Note: Auto lighting is optimal for producing accurate results, but if needed, can be disabled by
adjusting the lighting slider.
5.
(Optional) : Set exposure using the light source slider.
The light source slider controls the LED intensity, camera gain and exposure time, and allows you
to adjust the image brightness.
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Countess ™ 3 FL Automated Cell Counter User Guide