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5
ChromQuest Diagnostics for the PDA Detector
Calibrating the PDA Detector from the ChromQuest Data System
74
Accela PDA Detector Hardware Manual
Thermo Scientific
The PDA detector briefly turns the lamps off as it performs the dark current calibration
routine. After it completes the dark current calibration, the PDA detector turns the lamps
back on.
Y
To perform a dark current calibration of the diode array
1. Prepare the PDA detector for calibration (see
“Preparing the PDA Detector for
2. Open the Diagnostics dialog box for the Accela PDA Detector (see
Controls for the Accela PDA Detector”
).
3. Click the
Calibration
tab.
The Calibration page appears.
4. In the Array area, click
Execute
.
A message box appears (see
Figure 61.
Calibration preconditions dialog box
5. Make sure that the lamps are warmed up, that the pump is running at 1 mL/min, and
that the filter wheel is in position 1 (see
), and then click
OK
.
The status of the calibration procedure appears by the Status readback area on the
Calibration page. During the dark current calibration, the lamps turn off before the data
system collects the intensity scans. After the last calibration event, the lamps turn back on.
6. Click
OK
to finish the calibration.
The date and time of the calibration appear in the Array area of the Calibration page and
are stored in the PDA detector’s memory.
Note
The dark current calibration program does not run when data collection is enabled
on the Display page.