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Horizontal System

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Thermo Scientific

Table of Contents

General Information  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Setting Up  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Preparing the Gel  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Using the System  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Finishing Up  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Specifications and Recommended Running Conditions . . . . . . . . . . .4-1

Technical Tips  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Combs  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Reagent Information  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Troubleshooting  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Care and Cleaning  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Optional Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Replacement Parts  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Section 1

Section 2

Section 3

Section 4

Section 5

Section 6

Section 8

Section 7

Содержание A2-OK

Страница 1: ...Horizontal System Model A2 OK Operating and Maintenance Manual 7219011 Rev 0 Visit us online to register your warranty www thermoscientific com warranty ...

Страница 2: ...Thermo Scientific MANUAL NUMBER 7219011 0 4 13 12 Transfer to Marietta was 11 2002 ccs REV ECR ECN DATE DESCRIPTION By Preface Horizontal System i ...

Страница 3: ...product only for its intended purpose as described in this manual Do not use this product if the power leads are damaged or if any of its surfaces are cracked Warning Running conditions for this unit should not exceed the name plate readings found on the lower buffer chamber s This Owl System is designed to meet IEC 1010 1 safety standards IEC 1010 1 is an internationally accepted electrical safet...

Страница 4: ...equipment that has been put on the market after 13 August 2005 This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled ...

Страница 5: ...n fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are cor...

Страница 6: ...2 2 Using the System 3 1 Finishing Up 4 1 Specifications and Recommended Running Conditions 4 1 Technical Tips 5 1 Combs 5 1 Reagent Information 5 2 Troubleshooting 6 1 Care and Cleaning 7 1 Optional Equipment 8 1 Replacement Parts 8 1 Section 1 Section 2 Section 3 Section 4 Section 5 Section 6 Section 8 Section 7 ...

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Страница 8: ...design allows the casting of gels without tape or plugs UV transmissible gel trays may be placed directly on the transilluminator for photodocumentation Before starting unpack the unit and inventory your order If any parts are missing contact Technical Services immediately Reference the order or catalog number on your invoice and check the corresponding parts list Table 1 1 Parts List Buffer Chamb...

Страница 9: ... Parts Diagram Table 1 1 Specifications Gel size 7cmW x 8cmL Running Buffer Volume 2 3L Footprint 28cm W x 37 5cm L x 10cm H Power Supply Leads Super Safe Lid UVT Gel Tray 6 Gel Tray Holder Buffer Chamber Bubble Level Double Sided Comb 12 Multiple Gel Caster 2 Gasket 12 1 5 Thick 1 0 Thick ...

Страница 10: ...ong the side of the gel caster 3 Prepare and pour warm 60 agarose solution onto UVT gel tray Place comb s into slots provided on walls of UVT gel tray 4 One the gel has solidified place the UVT gel tray s into the gel tray holder 5 Level the casting chamber using the thumbscrews on each side of the unit by slowly turning one thumbscrew at a time and lining up the bubble in the level with the cente...

Страница 11: ...elow 60 before casting to avoid warping the UVT gel tray due to excessive heat If numerous gels are to be run in one day a large volume of gel may be prepared and placed in a covered bottle stored between 40 60 in a water bath This provides a ready gel supply in a warm liquid form that will solidify quickly when gels are cast Pour or pipet warm agarose 60 Immediately after pouring insert the desir...

Страница 12: ... Pour enough compatible running buffer into the unit to fill the chamber and completely cover and submerge the gel Correct buffer level is clearly marked on the units side wall as FILL LINE See Recommended Running Conditions for approximate buffer volumes needed for your unit Too little buffer may cause the gel s to dry out during the run while excess buffer may decrease DNA mobility and cause ban...

Страница 13: ...he gel tray into the gel tray holder in the running position with the gel edges facing out toward the buffer chambers and slowly fill as described in Step 2 It is wise to always run a sample lane of a known standard ladder to determine concentration and size of separated fragments after the gel run and to aid in photo documentation and analysis Note It is sometimes easier to load the sample wells ...

Страница 14: ...of agarose commerically available that may be used Besides standard ultra pure electrophoresis grade agarose there are also numerous low melting point products for easy sample recovery as well as speciality products formulated for specific uses to separate recover very small or very large fragments etc To visualize and photograph the samples after the gel run for a permanent record the gel may be ...

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Страница 16: ...le Sided Double Sided Molded Combs combine 1 0mm and 1 5mm tooth thickness on one comb Preparative One long well and one marker lane Wall Comb See page 33 for details Multi Load Comb F or use with 8 channel pipet Custom Combs Call Technical Services for more information Standard Double Sided Preparative Wall Comb Multi Load Comb ...

Страница 17: ... to be more difficult to separate visualize and photograph A specialty agarose product formulated to increase resolution of low molecular mass samples may also be used Example A good mid range gel percentage would be 0 7 or 0 7g agarose in 100mls electrophoresis buffer TBE or TAE following heating and dissolving the agarose 10ul of ethidium bromide stock solution 5mg ml is added The gel would be r...

Страница 18: ... 108 g Tris base 89 mM Tris base 55 g boric acid 89 mM boric acid 7 44g Na2EDTA 2H2O MW 372 24 2 mM EDTA or 40 ml 0 5 M EDTA pH 8 0 Distilled H2O to 1 liter final volume Do not adjust pH Buffer Suggested Uses and Comments TAE Buffer Use when DNA is to be recovered For electrophoresis of large 20kb DNA Applications requiring high resolution Has low ionic strength and low buffering capacity recircul...

Страница 19: ...ing formula and may be adjusted according to need or preference gel width cm x gel length cm x gel thickness cm ml of agarose Agarose Gel Loading Buffer Samples are prepared and combine with gel loading buffer before applying to the prepared gel Sample buffer usually contains similar components to the running buffer dyes for visibility and glycerol to provide some weight to the samples This increa...

Страница 20: ... gel tray holder on the platform and cool the agarose to below 60 before pouring to avoid warping the UVT gel tray s Samples seem to be running unevenly in certain areas Check that the platinum electrode wire is intact running flat and evenly across the outer corners and up the side to the junction of the banana plug area This problem could also be caused by regular casting with very hot agarose g...

Страница 21: ...atinum wires on both sides of the unit should pro duce small bubbles as the current passes through If a complete circuit does not exist there will be few to no bubbles Contact Technical Services to schedule a repair When the comb is removed from the gel some sample wells are ripped and damaged Always make sure to allow the gel to solidify completely before moving the gel tray unit or removing the ...

Страница 22: ...ogy A Compendium of Methods from Current Protocols in Molecular Biology Edited by Fredrick M Ausubel et al Adams D and R Ogden Electrophoresis in Agarose and Acrylamide Gels Methods in Enzymology Vol 152 1987 Academic Press Inc Fotador U Simultaneous Use of Standard and Low Melting Agarose for the Separation and Isolation of DNA by Electrophoresis BioTechniques Vol 10 No 2 1991 Boots S Gel Electro...

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Страница 24: ... Away Spray wipe or soak labware with RNase Away then wipe or rinse the surface clean it instantly eliminates RNase RNase Away eliminates the old methods that include treatment with 0 1 Diethyl Pyrocarbonate DEPC treated water and soaking in diluted bleach This electrophoresis system should never be autoclaved baked or placed in a microwave Warning DEPC is suspected to be a carcinogen and should b...

Страница 25: ...s found in the biochemical laboratory some solvents can cause substantial damage Keep this chart handy to avoid harm to your apparatus by the use of an inappropriate solvent Codes S Safe No effect except possibly some staining A Attacked Slight attack by or absorption of the liquid Slight crazing or swelling but acrylic has retained most of its strength U Unsatisfactory Softened swollen slowly dis...

Страница 26: ...asoline regular leaded S Phenol 5 solution U Hydroxide 10 S Glycerine Heptane commercial grade S Soap solution Ivory S Ammonium Hydroxide concentrate S Hexane S Sodium carbonate 2 S Aniline D Hydrochloric acid 10 S Sodium carbonate 20 S Benzene D Hydrochloric acid concentrate S Sodium chloride 10 S Butyl Acetate D Hydro uoric acid 40 U Sodium hydroxide 1 S Calcium chloride saturated S Hydrogen per...

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Страница 28: ... Technical Services to order replacement parts Table 8 2 Replacement Parts Cat No Description PSL 5 Power Supply Leads B1A UVT Gasketed UVT gel tray B1A CST Multiple Gel Caster A2 OT Gel Tray Holder B1A GK Replacement Gaskets 1 pair Combs Horizontal System 8 1 Thermo Scientific Catalog of teeth Tooth Width mm Thickness mm B1A 5C 5 11 1 0 B1A 5D 5 11 1 5 B1A 6 6 9 1 5 1 0 B1A 8 8 6 1 5 1 0 B1A 10 1...

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Страница 30: ... component part beyond the original warranty period The Technical Services Department must give prior approval for return of any component or equipment At Thermo s option all non conforming parts must be returned to Thermo postage paid and replacement parts are shipped FOB destination THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES WHETHER WRITTEN ORAL OR IMPLIED NO WARRANTIES OF ME...

Страница 31: ... to the component part beyond the original warranty period The Technical Services Department must give prior approval for return of any component or equipment At Thermo s option all non conforming parts must be returned to Thermo postage paid and replacement parts are shipped FOB destination THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES WHETHER WRITTEN ORAL OR IMPLIED NO WARRANTIE...

Страница 32: ...Thermo Fisher Scientific 401 Millcreek Road Marietta Ohio 45750 United States www thermofisher com ...

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