background image

Prepare samples using MicroAmp

 tube strips with

attached caps

1.

Separate the 

blue

 tray and retainer by squeezing the release

catch as indicated in the graphic.

1

2

3

1

1

Release catch

2

MicroAmp

 96-Well Retainer

3

MicroAmp

 96-Well Tray

2.

Place the 

blue

 tray on the 96-well base.

3.

Load the tube strips on the tray.

4.

Pipette the reaction mixture into the tubes.

5.

Seal the tube strip using a MicroAmp

 cap strip. See “Seal

tubes strips with cap strips” on page 6 for instructions.

6.

Remove the 

blue

 tray containing the sealed tube strips from

the 96-well base and place the tray and sealed tube strips

into the instrument.

1

MicroAmp

 8-Tube Strip with Attached Caps (0.2-mL)

2

MicroAmp

 96-Well Tray

3

MicroAmp

 Splash Free 96-Well Base

Seal tubes strips with cap strips

IMPORTANT! 

Apply significant downward pressure on the

sealing tool in all steps to form a complete seal on top of the
tubes.

1.

Align and place the cap strips on the tubes.

2.

Seal the cap strips using the rocking capping tool:

a.

Slip your fingers through the handle with the holes in

the tool facing down.

b.

Place the holes in the tool over the first eight caps in a

row.

c.

Rock the tool back and forth a few times to seal the

caps.

d.

Repeat for remaining caps in the row, then for all

remaining rows.

Prepare samples using MicroAmp

 Reaction Tubes

1.

Set the 

black

 tray on a 96-well base.

2.

Place the reaction tubes in the 

black

 tray.

1

2

3

1

MicroAmp

 Reaction Tube with Cap (0.2-mL)

2

MicroAmp

 96-Well Tray for VeriFlex

 Blocks

3

MicroAmp

 Splash Free 96-Well Base

3.

Pipette the reaction mixture into the reaction tubes.

4.

Cap the tubes.

5.

Remove the 

black

 tray with sealed reaction tubes from the

96-well base and place the tray and sealed tubes into the

instrument.

6

ProFlex

 PCR System Installation and Operation Quick Reference

Содержание 4483636

Страница 1: ...protective devices is recommended Power line regulator 1 5 kVA Surge protector line conditioner 10 kVA Uninterruptible power supply 1 5 kVA Set up the ProFlex Thermal Cycler IMPORTANT Save the packing materials and box in case you need to ship the instrument in for service 1 Open the shipping crate to unpack the ProFlex Thermal Cycler The instrument is contained in three boxes one with the base mo...

Страница 2: ...ent Ethernet port using a cable Connect via wireless connection with the USB enabled Wi Fi Module Cat No A26774 Insert the Wi Fi Module into the USB port at the back of the instrument For instructions on setting up the wireless connection see How to set up the wireless connection for the ProFlex Thermal Cycler on page 3 1 USB WiFi port 2 Ethernet port 10 Press the power button then wait for the in...

Страница 3: ...k Sign up now and follow the prompts to create an account Link the PCR instrument to Connect Administrator only The procedure to link the Administrator profile to Connect only needs to be performed the first time the PCR instrument is added to Connect 1 Press Sign In Sign In then select the Administrator profile 2 Log in The default password for the Administrator profile is password last four digi...

Страница 4: ... heated cover can be as high as 110 C and the temperature of the sample block s can be as high as 100 C Before performing the procedure keep hands away until the heated cover and sample block s reach room temperature To ensure proper operation Regularly Wipe the instrument surfaces with a lint free cloth Clean the vents touchscreen and sample block of the instrument Clean the sample wells with 100...

Страница 5: ...croAmp single tubes or tube strips with separate cap strips The blue tray by itself is compatible for use with MicroAmp tube strips with attached caps The black retainer is compatible for use with single MicroAmp reaction tubes with attached caps Prepare samples using MicroAmp tubes tube strips with separate cap strips 1 Separate the blue tray and retainer by squeezing the release catch as indicat...

Страница 6: ... downward pressure on the sealing tool in all steps to form a complete seal on top of the tubes 1 Align and place the cap strips on the tubes 2 Seal the cap strips using the rocking capping tool a Slip your fingers through the handle with the holes in the tool facing down b Place the holes in the tool over the first eight caps in a row c Rock the tool back and forth a few times to seal the caps d ...

Страница 7: ...ure uniformity 0 5 C 20 seconds after reaching 95 C Dimensions Height 27 2 cm 10 7 in Width 33 0 cm 13 in Depth 56 5 cm 22 in Weight 18 8 kg 41 lb 20 4 kg 45 lb PCR volume range 10 80 µL 10 100 µL 33 nL 5 20 µL Instrument memory USB onboard Display interface 8 4 inch color TFT LCD Power 100 240 V 50 60 Hz Max 950 VA Note For Australia AU New Zealand NZ only 230 VAC 50 Hz Max 950 W Max Current 10 A...

Страница 8: ...late Optical 384 Well Clear Reaction Plates with Barcode 4483285 4483273 Strip tubes and caps MicroAmp Fast 8 Tube Strip 0 1 mL 4358293 MicroAmp Optical 8 Tube Strip with Attached Optical Caps 0 2 mL A30588 MicroAmp Optical 8 Tube Strip with Attached Domed Caps 0 2 mL A30589 MicroAmp 8 Tube Strip 0 2 mL N8010580 MicroAmp Optical 8 Tube Strip 0 2 mL 4316567 MicroAmp 8 Cap Strip clear N8010535 N8011...

Страница 9: ...n this guide is subject to change without notice DISCLAIMER TO THE EXTENT ALLOWED BY LAW THERMO FISHER SCIENTIFIC INC AND OR ITS AFFILIATE S WILL NOT BE LIABLE FOR SPECIAL INCIDENTAL INDIRECT PUNITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING YOUR USE OF IT Important Licensing Information These products may be covered by one or more Limited Use La...

Отзывы: