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Dionex PDA Operator’s Manual
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Doc. 065378-01 1/12
spectra are automatically collected as each peak elutes. The detector
software compares the spectra with those stored in a library to
determine the best fit matches; this method increases the likelihood of
correctly identifying peaks.
For example,
shows an overlay of pyrene and an impurity
obtained from two consecutive chromatographic analyses. Based on
2D data, or if the analyses had been performed with a conventional
variable wavelength detector, the peaks might have been
misidentified as the same component.
The Dionex PDA, under the control of Chromeleon 6.8 with the 3D
Data Acquisition license, correctly identified and differentiated the
components. The spectra of pyrene and the impurity (see
clearly indicate that the two peaks are different components.
Figure 1-2. Overlay of Pyrene and an Impurity
Impurity
Pyrene
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