Chapter 4 – Using the Instrument
•
Enter the required Total Measurement Time in Serial,
Multi-wavelength and Difference modes. In Parallel mode, the
Total Measurement Time is displayed but cannot be edited.
•
In Multi-wavelength, Difference and Parallel modes, enter the cycle
time.
•
If a delay is required between the start of the run and the first
measurement, enter a delay time.
•
Choose whether to display the absolute values of the absorbance, or
the change in the absorbance relative to the initial measurement. If
Relative absorbance is chosen, an additional menu item appears to
set positive or negative slope.
•
Set up Graph High and Graph Low to accommodate the expected
absorbance range. The graph can be reconfigured post-run, if
required.
•
Enter a factor and units, if required.
•
Smoothing may be specified at run time or applied post-run.
•
Save the new method into the instrument Library or to disk. Press
the Save Method function key and complete the save dialog as
required.
3.
Load a previously saved method from the instrument Library or
disk.
Press the Load Method function key, select the required file and load it.
4.
Clear the beams or insert appropriate blank(s) and close the lid of
the sample compartment.
5.
Press Zero.
6.
Load the cell holder, initiate the reaction(s) as necessary and close
the lid of the sample compartment.
7.
Press Run.
Thermo Electron Corporation
Evolution 300 and Evolution 600 User’s Guide
47