Terumo BCT Quantum Cell Expansion System, Руководство оператора

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Harvest Cells
The purpose of this task is to transfer cells in suspension from the IC circulation loop, including cells in
the bioreactor, to the harvest bag.
Table 12-12 describes the types of solution that are needed to attach to each line when performing
Harvest Cells. These solutions and corresponding volumes are based on the factory default settings for
this task.
Note: When harvesting cells, you should adjust the media composition in the harvest bag to ensure that it is
appropriate for the way in which you intend to use the cells after harvest.
Table 12-12: Solutions for Harvest Cells
Line
Solution
Attached to the
Line
Required Volume (estimate based on factory default settings)
Cell None N/A
Reagent None N/A
IC Media Harvest media* 0.4 L
Wash None N/A
EC Media Media without
protein 0.1 L
*When harvesting non-adherent cells, you can use PBS or a similar electrolyte solution as the harvest media; in this situation the harvest
media does not have to consist of a media formulation, because there is no trypsin to neutralize.
Harvest Cells pathway:
Task
>
Release and Harvest
>
Harvest Cells
1.
Confirm the settings for Harvest Cells shown in Table 12-13.
Table 12-13: Settings for Harvest Cells
Setting Factory Default Setting Options
IC Inlet IC Media Reagent, IC Media, Wash, EC Media
IC Inlet Rate 400 mL/min 100 to 500 mL/min
IC Circulation Rate −70 mL/min −87 to −18 mL/min
The system automatically calculates this rate based on the IC Inlet Rate
for this task.
EC Inlet EC Media Reagent, IC Media, Wash, EC Media
EC Inlet Rate 60 mL/min 0 to 100 mL/min
Harvest Cells
Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1 12-9