Add Fluid Continuously With Ultrafiltration
The purpose of this task is to continuously add fluid at a low flow rate to the IC circulation loop and/or
the EC circulation loop. If you use the factory default settings for this task and the solution volumes
based on those defaults, large molecules could become concentrated in the IC circulation loop. In this
instance, the task would use ultrafiltration to remove excess fluid from the IC side of the bioreactor.
Table 11-12 describes the types of solution that are needed to attach to each line when performing Add
Fluid Continuously With Ultrafiltration. These solutions and corresponding volumes are based on the
factory default settings for this task.
Table 11-12: Solutions for Add Fluid Continuously With Ultrafiltration
Line
Solution Attached
to the Line
Required Volume (estimate based on factory default
values)
Cell
None
N/A
Reagent
None
N/A
IC Media
Media with protein
6 mL/hour
Wash
None
N/A
EC Media
None
N/A
Add Fluid Continuously With Ultrafiltration pathway:
Task
>
Feed and Add
>
Add Fluid Continuously
With Ultrafiltration
1.
Confirm the settings for Add Fluid Continuously With Ultrafiltration shown in Table 11-13.
Table 11-13: Settings for Add Fluid Continuously With Ultrafiltration
Setting
Factory Default Values
Setting Options
IC Inlet
IC Media
Cell, Reagent, IC Media, Wash, EC Media, None
IC Inlet Rate
0.1 mL/min
0 to 10 mL/min
IC Circulation Rate
20 mL/min
−100 to 100 mL/min
The system automatically calculates this rate based on the IC Inlet Rate
for this task.
EC Inlet
None
Reagent, IC Media, Wash, EC Media, None
EC Inlet Rate
0 mL/min
0 to 10 mL/min
EC Circulation Rate
30 mL/min
10 to 300 mL/min
Outlet
EC Outlet
N/A
Feed and Add Tasks
11-12
Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1
Содержание Quantum Cell Expansion System
Страница 1: ...Quantum Cell Expansion System SOFTWARE VERSION 2 1 Operator s Manual...
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Страница 10: ...1 Preface Quantum Cell Expansion System Operator s Manual for Software Version 2 1 1 1...
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Страница 74: ...6 Performing Tasks Quantum Cell Expansion System Operator s Manual for Software Version 2 1 6 1...
Страница 96: ...7 Set Management Tasks Quantum Cell Expansion System Operator s Manual for Software Version 2 1 7 1...
Страница 110: ...8 System Management Tasks Quantum Cell Expansion System Operator s Manual for Software Version 2 1 8 1...
Страница 120: ...9 Washout Tasks Quantum Cell Expansion System Operator s Manual for Software Version 2 1 9 1...
Страница 130: ...10 Load and Attach Tasks Quantum Cell Expansion System Operator s Manual for Software Version 2 1 10 1...
Страница 141: ...Load and Attach Tasks 10 12 Quantum Cell Expansion System Operator s Manual for Software Version 2 1...
Страница 142: ...11 Feed and Add Tasks Quantum Cell Expansion System Operator s Manual for Software Version 2 1 11 1...
Страница 155: ...Feed and Add Tasks 11 14 Quantum Cell Expansion System Operator s Manual for Software Version 2 1...
Страница 156: ...12 Release and Harvest Tasks Quantum Cell Expansion System Operator s Manual for Software Version 2 1 12 1...
Страница 166: ...13 Custom Tasks Quantum Cell Expansion System Operator s Manual for Software Version 2 1 13 1...
Страница 168: ...14 Quantum System Configuration Quantum Cell Expansion System Operator s Manual for Software Version 2 1 14 1...
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Страница 172: ...15 Reports Quantum Cell Expansion System Operator s Manual for Software Version 2 1 15 1...
Страница 182: ...16 Maintaining the Quantum System Quantum Cell Expansion System Operator s Manual for Software Version 2 1 16 1...
Страница 185: ...Maintaining the Quantum System 16 4 Quantum Cell Expansion System Operator s Manual for Software Version 2 1...
Страница 186: ...17 System Specifications Quantum Cell Expansion System Operator s Manual for Software Version 2 1 17 1...