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UA-6 Detector
Section 5 Maintenance and Adjustment

5-20

5.7.3 Troubleshooting

The most common malfunctions that may occur during normal
operation of the flow cells are identified in Table 5-6, which iden-
tifies the problem, probable cause, and the solution.

5.7.4 Flow Cell Cleaning

Procedures

It is recommended that the flow cells and quartz windows (or
quartz rods for the variable pathlength) be cleaned before the
flow cell is used in a system. To obtain the best cleaning results,
remove the quartz windows using the procedures specified in
section 5.7.1. Purge the flow cell with a warm detergent solution
and then rinse with distilled water or appropriate organic
solution. Oily deposits in a flow cell tend to aggravate the
trapping of bubbles. Rinse with a non-polar solvent (e.g. hexane)
followed by a rinse with a polar solvent (e.g. methanol). If the
flow cell has been used with aqueous solutions, precede the
non-polar and polar solvent rinses with an aqueous alkaline
detergent solution rinse, followed by a distilled water rinse, then
the polar solvent rinse. This will usually remove contamination
that causes bubble adhesion.

Table 5-6 Flow Cell Troubleshooting

Problem

Probable Cause

Solution

1. Leakage across the
flow cell windows.

a. O-ring is damaged (Figure 5-5)

a. Replace with a new O-ring.

b. Window nut is loose.

b. Using the special window nut wrench sup-
plied (Figure 5-5), tighten the window nut
until it is snugged down.

c. If not items a or b above, a sealant is
required.

c. Apply fluorosilicone oil using the proce-
dures specified in Section 5.7.2.

2. Leakage around the
lead connectors or
nuts.

a. Lead connector is loose in the flow cell
body.

a. For medium pressure lead connectors,
loosen the nut and retighten the lead con-
nector and nut using the procedures speci-
fied in Section 3.5.

b. Nut is loose on the lead connector.

b. For medium pressure lead connectors,
tighten the nut using the procedures speci-
fied in Section 3.5.

c. Ferrule and/or tubing is deformed.

c. Disconnect tubing from the lead connector
and cut off defective part.

For medium pressure lead connectors,
install a new ferrule and reconnect using the
procedures specified in Section 3.5.

d. O-ring between the lead connector and
the flow cell body is defective. The prepara-
tive flow cell requires no O-ring when fluoro-
carbon lead connectors are used.

d. Disconnect the tubing and remove the
lead connector. Install a new O-ring.

For medium pressure lead connectors,
install the lead connector and tighten the nut
using the procedures specified in Section
3.5.

3. Bubbles in the flow
cell.

a. Flow cell is dirty.

a. Clean the flow cell using the procedures
specified in section 5.7.4.

b. Liquids require degassing.

b. Degas the liquids before they are entered
into the system.

c. Flow cell pressure is too low.

c. Install restriction in the flow cell outlet line.

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Содержание UA-6

Страница 1: ...UA 6 Absorbance Detector Installation and Operation Guide Part 69 0943 266 of Assembly 60 0943 261 Copyright 1991 All rights reserved Teledyne Isco Revision M October 2013 ...

Страница 2: ......

Страница 3: ...zation Number specified Be sure to include a note describing the malfunction This will aid in the prompt repair and return of the equipment Teledyne Isco welcomes suggestions that would improve the information presented in this manual or enhance the operation of the equipment itself Teledyne Isco is continually improving its products and reserves the right to change product specifications replacem...

Страница 4: ......

Страница 5: ...alerting you to dangerous voltage inside the product The exclamation point within the triangle is a warning sign alerting you to important instructions in this manual Ce symbole signale la présence d un danger d électrocution Ce symbole signale l existence d instructions importantes relatives au produit dans ce manuel ...

Страница 6: ...epfeilte Blitz im Dreieck ist ein Warnzeichen das Sie vor gefährlichen Spannungen im Inneren des Produkts warnt Das Ausrufezeichen in Dreieck ist ein Warnzeichen das Sie darauf aufmerksam macht daß wichtige Anleitungen zu diesem Handbuch gehören Esta señal le advierte sobre la importancia de las instrucciones del manual que acompañan a este producto ...

Страница 7: ...TED ENVIRONMENT DESIGNED FOR THESE TYPES OF MATERIALS IN ACCORDANCE WITH FEDERAL STATE AND LOCAL REGULATORY LAWS AND IN COMPLIANCE WITH YOUR COMPANY S CHEMICAL HYGIENE PLAN IN THE EVENT OF A SPILL IN ALL CASES WHEN USING TELEDYNE ISCO INC INSTRU MENTATION PRUDENCE AND COMMON SENSE MUST BE USED CAUTION If this instrument is used in a manner not specified in this manual the protection provided by th...

Страница 8: ...ET ENVIRONNEMENT ISOLÉ DEVRAIT ÊTRE CONSTRUIT SELON LES RÈGLEMENTS FÉDÉRAUX PROVINCIAUX ET LOCAUX AUSSI QUE LE PLAN DE VOTRE COMPAGNIE QUI CONCERNE L ÉVÈNEMENT D UN ACCIDENT AVEC LES MATIÈRES HASARDEUSES EN TOUT CAS UTILISEZ TOUJOURS L INSTRUMENTATION D ISCO AVEC PRUDENCE ET SENS COMMUN AVIS Si vous employez cet instrument d une manière autre que celles qui sont specifiées dans ce manuel la protec...

Страница 9: ...hart speed range x 0 049804867 UA 6 line frequency chart speed Example 100 X 10 300 cm hr 60 Hz 100 X 10 X 1 X 300 X 0 049804867 60 249 02343 cm hr Example 25 X 1 15 cm hr 50 Hz 25 X 1 X 1 X 15 X 0 049804867 50 0 3735153 cm hr CHART LENGTH PER UNIT VOLUME PUMPED Approximate cm ml FOR SINGLE CHANNEL OF PUMP Chart Speed cm hr 60 Hz Line Frequency 50 Hz Line Frequency 1 16 tubing 1 8 tubing 1 16 tubi...

Страница 10: ...nce Our staff will do its utmost in analyzing and solving any problems If you have developed a unique application for a Teledyne Isco Instrument or are considering a new use we would be interested in learning about it TRADEMARKS Amphenol is a Registered Trademark of BTC Electronic Components Halar is a Registered Trademark of Allied Corporation Hastelloy C is a Registered Trademark of Cabot Corpor...

Страница 11: ...pots etc within the quartz envelope are not detrimental to the function of the lamp Description Part Number UV lamp Hg vapor for Type 11 optical unit 60 0947 027 chart paper 69 0943 193 pens 68 0947 020 flow cell windows prep cell 10 mm diameter 60 0644 052 Type 11 filters 254 nm 60 1144 001 280 nm 60 1144 002 Type 11 source screens 254 nm 60 1144 065 280 nm 60 1144 008 flow cell connectors 68 184...

Страница 12: ...x ...

Страница 13: ...ow Cell Applications 3 2 3 2 1 Preparative Flow Cells 68 0080 001 002 034 3 2 3 2 2 High Flow Rate Cell 68 0080 023 3 2 3 3 Flow Cell Descriptions 3 2 3 3 1 Preparative Flow Cells 3 3 3 3 2 High Flow Rate Cell 3 5 3 4 Flow Pressure Characteristics 3 6 3 5 Tubing Connections for Plastic Lead Connectors 3 6 3 6 Tubing Connections for High Flow Rate Cell 3 7 3 7 Selecting Chromatography Flow Cells 3 ...

Страница 14: ...ector Circuit 4 10 Section 5 Maintenance and Adjustment 5 1 Removal and Installation of the Case Top 5 1 5 2 Part Replacement 5 1 5 2 1 Recorder Replacement 5 1 5 2 2 Recorder Drive String Installation 5 3 5 3 Timing Belt Tension Adjustment 5 4 5 4 Cleaning the Enclosures 5 5 5 5 Troubleshooting 5 5 5 6 Type 11 Optical Unit Maintenance 5 11 5 6 1 Type 11 UV Lamp Replacement 5 11 5 6 2 Regulating P...

Страница 15: ...uit for a Remote Chart Drive 3 19 3 14 Problem Analysis Drift and Noise 3 20 3 15 Example Traces for Troubleshooting Analysis 3 21 4 1 Optical Unit Light Path 4 1 4 2 UA 6 Detector Block Diagram 4 2 4 3 Type 11 Optical Unit Functional Diagram 4 9 4 4 Type 11 Optical Unit Schematic Diagram 4 10 4 5 Type 11 Absorbance Detector Block Diagram 4 11 5 1 Built In Recorder Removal and Installation 5 2 5 2...

Страница 16: ...Isco UA 6 Table of Contents xiv ...

Страница 17: ...e UA 6 detector consists of two components the detector with built in chart recorder variable noise filter and peak separator and separated by a six foot cable one of two optical units The optical units house the lamp filters and flow cells and are designed to be plugged into the UA 6 and attached to a ring stand mast or shelf 1 1 Optical Units for the UA 6 1 1 1 Type 11 The Type 11 optical unit i...

Страница 18: ...d high flow LC flow rates up to 3liters minute 1 2 2 Cuvette Reading The UA 6 can operate as a UV VIS computing colorimeter with optional cuvette holders and 10 mm cuvettes 1 3 Technical Specifications The technical specifications of the UA 6 are listed in Table 1 1 1 4 Controls and Indicators The controls and indicators utilized in the operation of the UA 6 are illustrated in Figures 1 2 and 1 3 ...

Страница 19: ...ximum deviation measured at mid point of 2 0 absorbance range Noise Specifications for the Type 11 Optical Unit 10 x 10 5 AU 280 nm 10 x 10 5 AU 254 nm Reference 8 x 10 5 ASTM All noise specifications are for 95 total trace with dry PREP flow cells and 1 5 second RISE TIME setting reference 1 5 cm hr chart speed Accuracy Relative 3 of full scale all ranges Range to Range Tracking 2 of reading Nois...

Страница 20: ...egative to zero slope inflection Event Mark Inputs Open collector or contact closure Recorder Event Mark Display Type Five to 10 full scale spike added to recorder signal Indication Downscale for fraction collector index upscale for accessory e g Foxy rack change Duration 1 2 second Maximum Altitudes 2 000 Meters Pollution Degree 2 Installation Category II Built In Recorder Chart Width 10 cm calib...

Страница 21: ... on graduated chart paper to indicate and record the light absorbency of a medium in the optical unit light path 3 PEAK SEPARATOR off 0 3 1 3 10 30 Rotary selector switch Turns peak separator on optimizes the peak sepa rator for various peak widths in minutes 4 CHART SPEED CM HR 0 3 0 6 1 5 3 6 15 30 60 150 300 Rotary selector switch Stops and starts recorder chart drive and selects speed ranges f...

Страница 22: ... positioning of the absorbance baseline zero 9 NOISE FILTER 0 5 1 5 5 0 3 position selector switch Reduces noise by employing a Bessel type active filter The 0 5 1 5 and 5 0 positions indicate the approximate theoretical response time in seconds for 10 to 90 percent full scale output deflection 10 Standby Operate LED Indicator lamp Lights to indicate that secondary power is applied to circuitry an...

Страница 23: ...it breakers only one is installed for 100 or 117 volt operation Limits the detector current drawn from the main power supply Push to reset 5 RECORDER SELECTOR 2 position slide switch Selects signal source for UA 6 recorder INT Operates from UA 6 absorbance signal EXT Recorder operates on an external 0 1V sig nal input 6 RECORDER OUTPUTS 1 V 10 mV common ground chassis ground Banana sockets Connect...

Страница 24: ...shielded pairs with a 24 pin connector Provides interface connection to a Teledyne Isco absorbance detector control system 2 BASELINE ADJUST MIN OPEN MAX OPEN Rotary Control Moves an aperture plate assem bly back and forth in the light path to meter the light for zero baseline calibration procedures 3 COLDROOM HEATER ON OFF Toggle Switch SPDT Applies and removes approxi mately 13 VDC to the UV lam...

Страница 25: ...3 Installing the Chart Paper and Pen To install the paper refer to Figure 2 1 1 Raise the pen lift arm so that the pen clears the roller 2 Unroll several inches of paper from a new roll of chart paper 3 Fold or cut the leading edge of the paper at a 30 angle Don t fold the paper into a point this tends to cause a snag 4 With the printed side of the paper away from you feed the paper up around the ...

Страница 26: ...fer to Figure 2 1 1 Slide the pen cartridge into its holder A dimpled clasp holds it in place 2 Remove the pen cap a press fit and lower the pen with the pen lift arm left of the roller to ready the pen for use Replace the pen cap on the pen when the recorder is not in use to prevent the tip from drying out 3 To remove the pen pull the cartridge straight out of the holder Figure 2 1 Recorder Chart...

Страница 27: ...it can be placed inside the coldroom and the detector left outside by passing the optical unit cable through a port and completing the connection An optional 20 foot extension cable P N 68 1147 017 is available for applications which require additional separation of the optical unit and detector Each optical unit has a special mounting clamp which can be used to clamp to a ring stand or mast or by...

Страница 28: ...t To change wavelength operation 1 Release the two white catches on the front of the unit and open the Type 11 and remove the wavelength filters from each side of the optical unit as shown in Figure 2 3 The BASELINE ADJUST control on the optical unit will have to be used to clear the access to the filter Remove the fil Table 2 1 Selectable Wavelengths for Type 11 Optical Unit Wavelength nm Bandwid...

Страница 29: ...e holder and tighten the thumbscrew 5 Return the source screen holder to the slot in the Type 11 Figure 2 4 Changing the Source Screen in the Type 11 Optical Unit Note Always connect flow cells so that the direction of flow is up 2 6 Installation of Flow Cells Select the correct flow cell for your application see Section 3 2 Using the two white latches on the front open the optical unit and instal...

Страница 30: ... 1 Note The UA 6 was calibrated at the factory such that any optical unit can be connected to it and the accuracy of the system maintained But when field adjusting R224 the UA 6 will only be accurate with the optical unit with which it was calibrated Figure 2 5 Installing Flow Cells 2 8 Baseline Adjustment for Absorbance For baseline adjustment when starting operation set the UA 6 controls as foll...

Страница 31: ...PTICS to desired absorbance range 9 Align RECORDER OFFSET control to it s mark Activate AUTO BASELINE The pen should deflect near mid scale 10 The baseline setting may be left at mid scale or moved to any arbitrary baseline by using the recorder offset control of the UA 6 Positioning the baseline will not alter the accuracy of the reading 11 The AUTO BASELINE function can be activated by an extern...

Страница 32: ...UA 6 Detector Section 2 Preparation for Use 2 8 ...

Страница 33: ...he LED should not change from green to red An ambient temperature change of more than 15 C or a weak lamp are generally the cause of lamp current to go out of regu lation After an intermittent power loss if the UA 6 power LED does not return to green see Section 4 for adjusting lamp current Should the UA 6 power LED flicker red green this indi cates the 1140 lamp is also flickering See Table 5 1 S...

Страница 34: ...pressure The cell has a 5 mm pathlength 3 3 Flow Cell Descriptions For certain applications it may be necessary to change the O ring in your flow cells for increased solvent resistance Use the solvent compatibility chart Table 3 1 to determine which O ring will best suit your application requirements For a free guide to solvent compatibilities order a copy of Teledyne Isco Tables Table 3 1 O Ring ...

Страница 35: ... Operation with the included optional rectan gular aperture is recommended when maximum resolution of small volume peaks is required If the rectangular aperture is used make certain the slit is aligned so it is perpendicular to the flow The lead connectors are fabricated from the same fluoroplastic the nuts are fabricated from an acetal plastic and the 1 8 inch 3 2 mm ferrule and 1 16 inch 1 5 mm ...

Страница 36: ... Part Number 68 0080 001 68 0080 002 68 0080 034 68 0080 023 Pressure Rating 200 psi 14 kg cm2 200 psi 14 kg cm2 400 psi 28 kg cm2 200 psi 14 kg cm2 Tubing OD Accomodates 1 8 or 1 16 inch steel plastic or glass Accomodates 1 8 or 1 16 inch steel plastic or glass Accomodates 1 8 or 1 16 inch steel plastic or glass Accomodates 3 8 or 1 4 inch flexible or rigid plastic Standard O Ring Material Viton ...

Страница 37: ... preparative cells but has a 1 8 inch flow path This cell is capable of working with flow rates up to 3 liters per minute Compression fittings made of stainless steel and polypropylene are provided with the fixed pathlength flow cell and will accom modate 3 8 inch O D or 1 4 inch O D plastic tubing Figure 3 3 Flow Cell Flow Pressure Characteristics ...

Страница 38: ... 8 or 1 16 inch OD plastic or steel tubing with the fol lowing procedure see Figures 3 4 3 5 and 3 6 1 If the flow cell has been disassembled for cleaning install the lead connector in the flow cell body and finger tighten 2 Slide the nut over the end of the tubing 3 Slide the ferrule over the end of the tubing Ensure that the larger diameter of the conical shaped ferrule is facing the nut 4 Inser...

Страница 39: ...cedure and refer to Figure 3 6 1 Push the tubing through the threaded ferrule nut 2 If 3 8 inch OD x 1 4 inch ID flexible tubing is being used push the tubing insert into the end of the tubing 3 Insert the tubing into the lead connector and tighten the threaded ferrule nut CAUTION Use only finger pressure to tighten the lead connector in the flow cell body during step 4 below Over tightening can r...

Страница 40: ...y For most purposes an intermediate pathlength is better because of smaller volume and in the case of preparative flow cells better liquid flow characteristics Nonlinear response due to detector bandwidth diminishes greatly as absorbance decreases Therefore photometric linearity can be improved with shorter pathlength cells which require high instrument sensitivities lower absorbance ranges for th...

Страница 41: ...UA 6 Detector Section 3 Operation 3 9 Figure 3 6 Ferrule Application Information ...

Страница 42: ...hrough the detector 3 7 3 Preparative Cells In low pressure liquid chromatography where the peak volume of eluted components is large over 1 ml preparative flow cells are best These cells have a straight through vertical flow design to quickly expel trapped bubbles due to outgassing The larger illu minated volumes of preparative flow cells do not affect the intensity resolution or the shape of lar...

Страница 43: ...et tings of 0 5 1 5 and 5 0 seconds RISE TIME is a response speed measurement defined as the time required to make a 10 to 90 pen deflection This filter can be used to reduce the noise level on the recorder trace It is especially useful for reducing or elimi nating flow noise caused by pulsatile pumps or noisy solvents see Figure 3 7 The figure demonstrates the ability of the noise filter to reduc...

Страница 44: ...ve and not an absorbance threshold or level The peak separator is designed to collect all peaks regardless of their degree of resolution 3 11 1 PEAK SEPARATOR Switch Peak cutting with the peak separator is largely automatic The PEAK SEPARATOR switch is used to fine tune peak cutting based on the expected peak widths at baseline The peak sepa rator can most accurately respond to baseline peak width...

Страница 45: ...dvance one position When used with fraction collectors that include a delay timer the peak separator can be used to precisely collect peaks by synchro nizing the action of the fraction collector with the actual arrival of the peak at the drop outlet When used with fraction collectors without a delay timer satisfactory peak cutting will occur but the fraction collector will move slightly prematurel...

Страница 46: ...erformance is directly related to the stability of the ambient temperature The UA 6 can operate at any temperature between 0 C and 40 C without affecting drift as long as it remains stable Temperature changes of 1 C will cause noticeable drift at a SENSITIVITY setting of 0 002 AUFS Any step that tends to stabilize temperatures including insulating the system column tubing injection valve will help...

Страница 47: ...UA 6 Detector Section 3 Operation 3 15 Figure 3 9 Absorbance Curves of Four Commonly Used Solvents ...

Страница 48: ... Connection to Other Instruments The interface cables available from Teledyne Isco for use with other instruments are summarized in Table 3 3 This table can be used as an ordering guide for cables The full catalog order number is listed in the table The cables are tagged with a seven digit number The input and output signals handled by the UA 6 and its rela tionship to other instruments in a syste...

Страница 49: ...wing interface to the recorder output a dual banana plug or for peak separator input a 9 pin plug It is recommended that the UA 6 peak detector be switched to OFF and the Foxy R1 R2 peak separator be used instead Note Be sure the polarity of the black dual banana plug connector is correct Match the ridge or keel on the connector with the negative binding post on the detector Figure 3 11 Cable Conn...

Страница 50: ...hat mates with a Cinch Jones S 304 CCT plug or equivalent The pins of the plug are 1 50 or 60 Hz time base at 19 VAC 2 common 3 pulse input 4 15 VDC This plug can be used to stop the chart drive motor or to drive the motor at a variable rate Connect a manual switch across pins 1 and 2 The chart drive stops when the switch is closed Short pins 1 and 2 The recorder chart motor may be driven with lin...

Страница 51: ...be For a 50 Hz recorder chart travel will be 3 15 Converting the Internal Recorder for Stand Alone Operation Switch the recessed rear panel switch INT EXT to external Connect external signal source to the red INT REC socket and the black common socket 216000 recorder range cm hr pulses per cm 180000 recorder range cm hr pulses per cm ...

Страница 52: ...UA 6 Detector Section 3 Operation 3 20 Figure 3 14 Problem Analysis Drift and Noise ...

Страница 53: ...following traces represent various difficulties as each is noted All were run with a chart speed of 1 5 and 3 cm hr with sensitivity at 0 01 AUFS unless otherwise noted Figure 3 15 Example Traces for Troubleshooting Analysis See Table 5 5 Item 2 ...

Страница 54: ...UA 6 Detector Section 3 Operation 3 22 ...

Страница 55: ...l the necessary elec tronics to drive the optical unit and prepare its light sensitive signals to be recorded on a chart 4 1 1 Optical Unit All current UA 6 optical units are dual light path devices which use two flow cells as depicted in Figure 4 1 The optical units all operate with a single light source which emits the desired ultra violet and visible spectrum When this light is passed through t...

Страница 56: ...y or refraction changes are reduced by this subtraction process thus making the system more stable The difference signal is converted to a logarithmic function The chart recorder output is the logarithm of the light intensity Next the signal is amplified and filtered as necessary At this point it is sent to the peak separation network and chart recorder With the help of any external signals that m...

Страница 57: ... clock the address register U201 203 204 up or down depending on the signal received from U205C and U205D U205C and U205D monitor the absorbance signal from the loga rithmic amplifier to determine which direction the circuit should count As the address register advances the voltage produced by the DAC U207 and buffer amplifier U210D U210B advances The measuring photocell in the optical unit is fed...

Страница 58: ...he U213 amplifier couples the absorbance signal from the logarithmic amplifier to the absorbance amplifier Absorbance amplifier U202B provides the sensitivity ranges based on the front panel switch position S5B 0 002 range pro vides a gain of 100 Gain decreases to 0 1 on the 2 0 range 4 2 4 Noise Filtering The noise filter U202A is a Bessel type time delay filter whose output is the time average o...

Страница 59: ...m 13 V to 13 V when a negative edge is detected Operational amplifier U404C switches from 13 V to 13 V when a positive edge is detected Capacitor C442 or C424 makes a pulse whenever U404D or U404C is turned on These pulses are used to index a fraction col lector when a new peak is detected or an old peak is finished U406A and Q414 produce a peak duration signal TTL low to P107 J217 during a peak 4...

Страница 60: ... latches the entire circuit Now the bias provided by U405 will remain fixed and the regulating photocell in the optical unit will maintain the lamp brightness Should the lamp current fall below 183 mA or exceed 260 mA after the 10 minute warm up cycle U402A will change the power LED from green to red This notifies the user that the lamp current is out of regulation and may require adjustment To re...

Страница 61: ...sed to connect the counter output for the desired chart speed to the other counter in U105 This counter divides by 5 or 6 depending on the position of the jumper P106 connected to pin 1 of U108A When operating on 60 hertz U108D and U107D provide 3 count high 3 count low 50 duty cycle pulse train to operate the stepper For 50 hertz operation U108B and U107C create a 21 2 count high 21 2 count low 5...

Страница 62: ...and REFERENCE CELL B light paths only one source screen is used UV light is reflected from the two reflectors and excites both sides of a light source which is part of the source screen resulting in a light emission spectrum at a certain wavelength region Depending upon the source screen used the emitted light is either diffused light from the UV lamp or is fluorescent light excited by the UV lamp...

Страница 63: ...n a decrease in the electrical resistance generated by the photodetectors Outputs from the photo detectors are transmitted to an absor bance detector control system which reflects these photodetector outputs as units of absorbance Figure 4 3 Type 11 Optical Unit Functional Diagram With respect to the 254 nm 280 nm 310 nm and 340 nm filter assemblies the UV lamp produces numerous discrete emission ...

Страница 64: ...cal density or light absorbance of a medium placed in the optical unit light path When signals from the logarithmic amplifier are applied to a linear inverting amplifier configuration the signals are inverted and the resultant output is a voltage which is propor tional to the optical density of the medium in the light path Any of the output signals from the optical unit may be used to drive read o...

Страница 65: ...UA 6 Detector Section 4 Theory of Operation 4 11 Figure 4 5 Type 11 Absorbance Detector Block Diagram ...

Страница 66: ...UA 6 Detector Section 4 Theory of Operation 4 12 ...

Страница 67: ... of the case Slide case top up and off 5 2 Part Replacement The following paragraphs provide instructions for replacement of parts requiring specific procedures The majority of replacement parts require soldering When removing electronic parts with multiple leads each lead should be labeled for identification prior to being disconnected Ensure that capacitors diodes and transistors are installed i...

Страница 68: ... not to damage any components within the detector 9 Carefully install the replacement recorder and all fasten ers previously removed 10 Connect P102 to the recorder circuit board 11 Insert the recorder speed control connector P104 onto the new recorder circuit board 12 Install the case top Figure 5 1 Built In Recorder Removal and Installation FRONT PANEL RECORDER SECURING NUT WASHER RECORDER 1 OF ...

Страница 69: ... T shaped stop bracket 2 Use a felt tipped pen to draw a realignment mark across the pulley and gear assembly hereafter referred to as the hub This mark ensures that the orientation of the shaft and the hub is maintained during reassembly Figure 5 2 Recorder String Routing SCALE 1 1 SCALE 2 1 REALIGNMENT MARK 1 16 SET SCREW STOP BRACKET B A D E C 1 OF 2 STOP BRACKET SECURING SCREW 1 OF 2 DELRIN WA...

Страница 70: ...g a slight tension If so secure the string to the spring then loosen the machine screw B and draw the ten sion from that end of the string Then retighten screw B 11 Install the T shaped stop bracket removed in step 1 and rotate the hub counter clockwise until the trapezoidal stop is against the stop bracket 12 Complete the string installation by threading the pen holder screw A back through the wa...

Страница 71: ... water For stains of an organic nature use iso propyl alcohol in a sponge that has been mostly squeezed dry 5 5 Troubleshooting The following sections identify the most common malfunctions that may occur during normal operation of the detector These tables identify the most commonly found problems and their causes and remedies A separate table is provided for each of the detector main systems or f...

Страница 72: ...ine procedure specified in Section 2 8 f Flow cell is dirty f Clean the flow cell following the procedure in Section 5 7 4 g Defective UV lamp in the optical unit g Replace the UV lamp following the proce dure in Section 5 6 1 h Defective filter cartridge in the optical unit h Check the filter cartridge following the pro cedure in Section 5 6 3 and if required replace it with a serviceable one i D...

Страница 73: ...LL B light path as in MEA SURING CELL A light path to improve the optical balance d Unstable UV lamp in the optical unit dur ing coldroom operation d Turn the COLDROOM HEATER switch to ON e Unstable UV lamp in the optical unit e Replace the UV lamp following the proce dure in Section 5 6 1 f Condensation on the flow cell windows f For the Type 11 optical unit turn the COLDROOM HEATER switch to ON ...

Страница 74: ...is in the full up position b Place the pen manual lift arm in the full down position 3 Pen does not respond to absorbance changes a INT EXT recorder switch set to EXT a Move switch to INT b The recorder 192 tooth gear is not secured to the feedback potentiometer shaft b Remove the recorder top cover and tighten the set screws securing the 192 tooth gear to the feedback potentiometer shaft c Pinion...

Страница 75: ... a Incomplete flushing of the flow cell a The major remedies are 1 Ensure that the flow cell is vertical and the effluent is moving upward through it 2 Use the shortest pathlength flow cell possible Larger pathlength flow cells have a greater volume which may decrease the resolution due to mixing in the flow path b Gradient elution b It is assumed that the correct baseline is the average of the ba...

Страница 76: ...tays red with a Type 11 opti cal unit connected a Defective UV lamp in the optical unit or misalignment of lamp regulating photocell a Replace or realign the UV lamp see Sec tion 5 6 1 b Defective electronic components b Using standard electronic techniques repair or replace parts as required 3 Automatic Peak Sep arator will not cause an Isco Fraction Collector to operate a Defective components in...

Страница 77: ...e area in accordance with local and federal statutes La lampe dans la cassette contient une très petite quantité de mercure ou de cadmium selon le type de lampe ces substances sont considérées comme toxiques Veuillez vous conformer à la législation en vigueur en particulier en cas de destruction de la lampe pour le nettoyage éventuel des zones souillées et la récupération éventuelle de la lampe ou...

Страница 78: ...ectors should have equal spacing on each side of the slot in the center bottom plate sub assembly so they are 3 16 inch apart and not touching the bulb at any point 10 Install and tighten screws 11 If required loosen the two securing screws securing the UV lamp socket assembly to the hinged end plate and move the UV lamp socket assembly to obtain clearance between the UV lamp and the reflector pla...

Страница 79: ... the center top plate sub assembly to the center bottom plate assembly and the hinged end plate 4 Grasp the center top plate sub assembly and gently pull toward you lifting the leading edge at the same time 5 Using a low wattage soldering iron disconnect the two wires to the regulating photocell assembly 6 Snap the defective regulating photocell assembly out of the mounting assembly 7 Snap the rep...

Страница 80: ...UA 6 Detector Section 5 Maintenance and Adjustment 5 14 Figure 5 4 UV Lamp and Regulating Photocell Removal and Installation ...

Страница 81: ...rom the photocell and filter holders and with the optical unit turned ON held up to the aperture in the reflector plate with the flanged end of the filter assembly towards the light source The filter assembly can be visually inspected by sighting through the opposite end and viewing the color emissions Source screens matching the filter assembly wavelengths must be installed in the optical unit fo...

Страница 82: ...2 1 b When two different wavelengths are in use there may be a difference in relative brightness b Reverse the cell locations of the matched source screen and filter assemblies c Flow cell is dirty c Clean the flow cell using the procedure specified in Section 5 7 4 d UV lamp in optical unit is defective d Replace the UV lamp using the procedures specified in Section 5 6 1 e The optical unit BASEL...

Страница 83: ...noise and drift are reflected in the indications j Make allowances for the normal noise and drift indications refer to Table 1 1 for detailed specifications k Fluctuations in pressure or flow rate through the flow cell are causing noise and drift problems Noticeable when using recip rocating pumps for high pressure in high speed chromatography in the 0 002 0 005 0 01 0 02 0 05 absorbance ranges k ...

Страница 84: ... install the replacement window in the flow cell body Note Refer to the Replacement Parts List in the back of this manual that is applicable to the flow cell in use to obtain the Teledyne Isco part numbers for the replacement items 5 Moisten the replacement O ring with distilled water and carefully install on top of the window 6 Carefully install the aperture insert applicable only to the preparat...

Страница 85: ...ect the O ring Figure 5 5 for signs of damage 3 If the O ring is damaged replace with a new one 4 If the O ring shows no signs of damage applicable to pre parative flow cells and high flow rate flow cells only apply a very small amount of fluorosilicone oil to the O ring CAUTION Use the fluorosilicone oil sparingly and DO NOT get any on the window area that is in the light path Fluorosilicone oil ...

Страница 86: ...nut is loose b Using the special window nut wrench sup plied Figure 5 5 tighten the window nut until it is snugged down c If not items a or b above a sealant is required c Apply fluorosilicone oil using the proce dures specified in Section 5 7 2 2 Leakage around the lead connectors or nuts a Lead connector is loose in the flow cell body a For medium pressure lead connectors loosen the nut and reti...

Страница 87: ... helpful in solving your problem Address your letter to Teledyne Isco Service Department P O Box 82531 Lincoln NE 68501 We suggest you call the Service Department first however before deciding to return the unit for factory repair Often a problem can be solved in the field with just a little extra help Our toll free number is 1 800 775 2965 5 7 7 How to Ship Returns Be sure all parts and hardware ...

Страница 88: ...available but there may be some delay in shipping them because they are not normally stocked The figures in the Replacement Parts section illustrate the immediate delivery items Each replacement part is given an item number which is shown in the callouts in the illustration and in the item number column of the list When ordering replacement parts please supply this information 1 series number of t...

Страница 89: ...er to the call out in the adjacent table to determine the part number for the item Replacement parts can be purchased by contacting Teledyne Isco s Customer Service Department Teledyne Isco Customer Service Department P O Box 82531 Lincoln NE 68501 USA Phone 800 228 4373 402 464 0231 FAX 402 465 3022 E mail IscoInfo teledyne com ...

Страница 90: ...UA 6 Detector Appendix A Replacement Parts A 2 ...

Страница 91: ...UA 6 Detector Appendix A Replacement Parts A 3 ...

Страница 92: ...UA 6 Detector Appendix A Replacement Parts A 4 MAIN CIRCUIT BOARD ...

Страница 93: ...UA 6 Detector Appendix A Replacement Parts A 5 ...

Страница 94: ...UA 6 Detector Appendix A Replacement Parts A 6 ...

Страница 95: ...UA 6 Detector Appendix A Replacement Parts A 7 ...

Страница 96: ...UA 6 Detector Appendix A Replacement Parts A 8 ...

Страница 97: ...UA 6 Detector Appendix A Replacement Parts A 9 ...

Страница 98: ...UA 6 Detector Appendix A Replacement Parts A 10 ...

Страница 99: ...UA 6 Detector Appendix A Replacement Parts A 11 ...

Страница 100: ...UA 6 Detector Appendix A Replacement Parts A 12 ...

Страница 101: ...UA 6 Detector Appendix A Replacement Parts A 13 ...

Страница 102: ...UA 6 Detector Appendix A Replacement Parts A 14 ...

Страница 103: ...UA 6 Detector Appendix A Replacement Parts A 15 ...

Страница 104: ...UA 6 Detector Appendix A Replacement Parts A 16 ...

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Страница 107: ...UA 6 Detector Appendix A Replacement Parts A 19 ...

Страница 108: ...UA 6 Detector Appendix A Replacement Parts A 20 ...

Страница 109: ...UA 6 Detector Appendix A Replacement Parts A 21 Drawing modified 7 6 11 to remove obsolete flow cells ...

Страница 110: ...UA 6 Detector Appendix A Replacement Parts A 22 ...

Страница 111: ...UA 6 Detector Appendix A Replacement Parts A 23 ...

Страница 112: ...UA 6 Detector Appendix A Replacement Parts A 24 ...

Страница 113: ...n 5 15 Flow Cells Applications 3 2 Descriptions 3 2 Installation 2 5 Front Panel 1 5 Fuses Type 12 2 4 I Installation Chart and Pen 2 1 L Leakage Flow Cell Window 5 19 N Noise and Drift Minimizing 3 13 Noise Filter 3 11 O Optical Unit Attachment 2 2 Optical Units 1 1 P Peak Separator 3 12 4 5 Power 2 1 Problem Analysis 3 20 R Rear Panel 1 7 Replacement Recorder 5 1 Recorder Drive String 5 3 Regula...

Страница 114: ...UA 6 Detector Index Index 2 Lamp Replacement 5 11 Theory of Operation 4 8 Type 12 Fuses 2 4 ...

Страница 115: ...t installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interfer ence at his own expense RADIO INTERFERENCE STATEMENT Canada This ISM apparatus meets all requirements of the Canadian Interferen...

Страница 116: ......

Страница 117: ...he concentration of the hazardous substance in this component s any homogeneous pieces is lower than the ST standard limitation X 嫷䯉年㦘㹡㦘䓸德咂年捷ↅ䤓㩟德㧟㠨䤓摞怔 ST 㪖屓䤓棟摞尐㻑ᇭ 㷳㫈㗽棔 ₙ嫷㓢 X 䤓㔏㦾扪嫛扪㷴広㢝ᇭ X Represent the concentration of the hazardous substance in this component s at least one homogeneous piece is higher than the ST standard limitation Manufacturer may give technical reasons to the X mark...

Страница 118: ......

Страница 119: ...EN61000 4 2 Electrostatic Discharge Level 2 4kV contact discharge Level 3 8kV air discharge B B EN61000 4 3 Radiated RF Immunity 80 MHz to 1000MHz 80 AM at 1kHz Level 1 1 V m A EN61000 4 4 Electrical Fast Transient Level 2 1kV on ac lines B EN61000 4 5 Surge on AC Lines 1kV common mode 500V differential mode B EN61000 4 6 Conducted RF on AC lines 150 kHz to 80 MHz 3V rms 80 modulated B EN61000 4 1...

Страница 120: ...Warranty ...

Страница 121: ...the instrument proves to be defective within 30 days of shipment Throughout the remainder of the warranty period the customer will pay to return the instrument to Teledyne Isco and Teledyne Isco will pay surface transportation to return the repaired instrument to the customer Teledyne Isco will not pay air freight or customer s packing and crating charges This warranty does not cover loss damage o...

Страница 122: ......

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