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4. Operating Instructions

4.3 Priming

Procedure

Before the instrument can be used, all dispensing channels must be primed
with the required solution.

Ensure that the bottle of priming solution is always full at the beginning of
the priming procedure and check that the inlet filters in the liquid tubes are
clear.

Note:

Please ensure that the dispensing and aspiration pumps are not run for

longer than a few minutes without liquid or they will be damaged.

The priming procedure is performed using the following procedure:

Run XX yyyyyyyy
- + other yes

Prime Ch1
- + other yes

Prime Solution ?
exit yes

Waste Bottle OK?
exit yes

Prime ChX Y sec
Stop

Run XX yyyyyyyy
- + other yes

Priming Procedure

performed

Select required channel to be primed

Waste bottle empty

Instrument ready to wash microplate

Required solution in bottle and inlet
tube inserted into solution

Priming procedure being performed

Caution

Do not use the instrument to aspirate or dispense any acidic solutions as

this could damage the instrument

May 2003

Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2

4-3

Содержание Columbus Washer

Страница 1: ...TECAN Instructions for Use for Columbus Washer Document Part No I 109 006 May 2003 Document Revision 2 2 Firmware Level 4 x...

Страница 2: ...Italy Tel 39 02 215 21 28 Fax 39 02 215 97 441 Japan Tecan Japan Co Ltd Meiji Seimei Fuchu Building 10F 1 40 Miyamachi Fuchu City Tokyo Japan Tel 81 42 334 88 55 Fax 81 42 334 04 01 Netherlands Tecan...

Страница 3: ...Ges m b H and are not to be copied reproduced or transferred to another person or persons without our prior written permission Copyright Tecan Austria Ges m b H All rights reserved Printed in Austria...

Страница 4: ...ally dangerous situation The following notices are Note Gives helpful information Caution Indicates a possibility of instrument damage or data loss if instructions are not followed WARNING INDICATES T...

Страница 5: ...2 3 3 Power Requirements 3 2 3 4 Installation Procedure 3 3 3 4 1 Installing the Instrument 3 3 3 4 2 Connecting the Solution Tubes 3 3 3 4 3 Fitting and Connecting the Liquid Sensors Optional 3 4 3...

Страница 6: ...6 5 3 1 Changing the Type of Defined Process Step 5 6 5 3 2 End Programming Procedure 5 6 5 4 Show Program 5 7 5 5 Clear Program 5 8 6 Adjust Menu 6 1 Introduction 6 1 6 2 Plate Type 6 3 6 2 1 Round...

Страница 7: ...se Day 7 11 7 5 3 Rinse Night 7 12 7 5 4 Rinse Auto 7 12 7 6 Instrument Disinfection 7 13 7 6 1 Disinfection Procedure 7 13 7 7 Disposal of Instrument 7 15 8 Trouble Shooting 8 1 Introduction 8 1 9 Se...

Страница 8: ......

Страница 9: ...e instructions may result in damage to the product injury to operating personnel or poor instrument performance 3 Observe all STOP and CAUTION statements in the IFU 4 Never open Columbus while the ins...

Страница 10: ......

Страница 11: ...nal drip mode setting is available which allows a wash buffer to be added drop by drop These features allow the instrument to be used for all ELISA microplate washing applications including Cell Washi...

Страница 12: ...384 Well Manifold 10 120 l in 10 l steps for dispensing Dispensing accuracy CV 4 over the plate 300 l dispensing volume dispensing rate 3 This is also for the 384 Well Manifold Residual volume Crosswi...

Страница 13: ...to 95 Others Overvoltage category II Pollution degree 2 Method of disposal Contaminated waste Linear Shaking Mode Shaking Speed 1 2 3 Low 1 2 mm 4 Hz 7 5 Hz 11 0 Hz Normal 1 7 mm 2 Hz 5 0 Hz 8 0 Hz Hi...

Страница 14: ...back panel Fig 2 2 Columbus Washer Rear Panel ON OFF Switch Fuse Compartment Mains Power Socket Printer Connector or Serial RS 232 for Remote Control Hand held Barcode Reader Bottle Holder with Liqui...

Страница 15: ...option Hand held Barcode Reader optional or Computer for Remote Control Waste Bottle Wash Liquid 1 Bottle Holders with liquid sensor for each bottle grey red green Rinse solution Marked red May 2003 I...

Страница 16: ...d thermal printer or EPSON compatible with 27 character per line and a power supply RS 232 interface 9600 Baud 8 data 1 stop no parity and hardware handshake BUSY on pin8 on the Washer connector Fill...

Страница 17: ...the Instructions for Use IFU and RS232 interface cable Firmware Update Consisting of the Download software the latest version of the firmware 3 x as well as 5 language software files English German F...

Страница 18: ...r Manifold Dispense Solenoid Valve Dispensing Pump Channel select Solenoid Valves Aspirating Pump Aerosol Pump optional Marked yellow Marked blue Marked red Marked yellow Marked red 2 8 Instructions f...

Страница 19: ...s left to stand or switched off Automatic Microplate Centering the microplate is automatically centered before the start of the washing program Programmable Strip Selection the program can be defined...

Страница 20: ...for the defined soaking time before proceeding to the next strip 2 5 1 Washing Modes The instrument can use the following washing modes to wash a microplate Overflow Washing For ELISA assays and Cell...

Страница 21: ...ped Wells With flat bottom wells the instrument performs crosswise aspiration The aspirating needles are set at two positions on the bottom of the wells front edge and back edge Fig 2 7 Crosswise Aspi...

Страница 22: ......

Страница 23: ...key Instructions for Use for Columbus Washer Mains power cable 2 Cleaning needles 2 spare fuses Solution tube 4m 3 spare 0 ring seals for manifold Bottle set containing 3 x 2 5 l bottles for the stan...

Страница 24: ...ent for loose bent or broken parts Report any damage immediately 6 Compare the serial number on the rear panel of the instrument with the serial number on the packing slip Report any discrepancy immed...

Страница 25: ...ssible instrument options See page 3 4 and 3 5 Connect instrument to the power supply See 3 4 6 Power Connections Define the plate parameters in the Adjust menu See chapter 6 Program the wash procedur...

Страница 26: ...or system can be extended to control up to four different liquid bottles and one waste bottle Fig 3 3 Connecting the Liquid Sensor Liquid Sensor waste red rinse grey liquid 1 green Fix the tube suppor...

Страница 27: ...e engineer Connect the printer to the instrument using the supplied cable Connect the power supply cable to the printer Fig 3 5 Connecting the Printer PAPER FEED BATT LOW POWER POWER OFF 1 2 ON Power...

Страница 28: ......

Страница 29: ...x is the instrument s software version If the Rinse Night rinsing procedure was not performed before the instrument was switched off the following message is displayed You should Rinse no yes Select i...

Страница 30: ...rform the selected program Priming procedure for each channel Rinsing procedure Selection of the Programming procedure to define the required program Procedure to clear a program from the instrument s...

Страница 31: ...will be damaged The priming procedure is performed using the following procedure Run XX yyyyyyyy other yes Prime Ch1 other yes Prime Solution exit yes Waste Bottle OK exit yes Prime ChX Y sec Stop Run...

Страница 32: ...d for the type of manifold that is fitted Fig 4 1 Fitting a Microplate 8 and 16 way Manifold as well as 384 Well Manifold 12 way Manifolds The operating procedure is dependent on the instrument option...

Страница 33: ...12 manifold has 8 Y Ns A 16 manifold has 12 Y Ns each representing 2 strips The 384 Well Manifold also has 12 Y Ns each representing 2 strips Select required strips using the cursor keys to selected...

Страница 34: ...No Barcode Reader Barcode Strip Read Read Barcode displayed for 2 secs Run XX yyyyyyyy other yes Print List now NO YES Printing When Procedure Completed Print setting NO Print setting YES Print settin...

Страница 35: ...e error messages appear if some of the needles are dispensing no or too little solution Possible errors could be a result of blocked dispensing needles bubbles in the dispensing channels empty liquid...

Страница 36: ...The conductance of the wash buffer must be between 5 mS cm and 30 mS cm The amount of wash liquid dispensed must produce at least 100 l overflow At least 450 l should be dispensed with an Overflow Pos...

Страница 37: ...Asp Speed 1 3 mm sec 384 Well Manifold Option This option is used for washing the 384 well microplate in rows The 384 Well Manifold performs both the cell washing and the ELISA washing procedure for...

Страница 38: ...manifold that is fitted If the correct type of manifold is fitted check the setting for the fitted manifold in the Adjust menu If either the Manifold Detection or Fill Verification option is enabled...

Страница 39: ...shing procedure Wastebottle Full yes Note The instrument does not stop a procedure when the waste bottle becomes full Therefore ensure that the waste full sensor is calibrated so that there is enough...

Страница 40: ...xample the side of the wells the following message is displayed Manif arm Error Exit Press exit to remove the error message and return to the standby mode Check that the microplate is correctly insert...

Страница 41: ...If the fill verification board needle status can not be deleted the following error message is displayed Fill Funct Err Exit Press exit to remove the error message and return to the standby mode The p...

Страница 42: ...nse the wash system See chapter 7 When the rinsing procedure is completed the following message is displayed Soaking Stop If the manifold is to be soaked overnight do not switch the instrument off Ins...

Страница 43: ...d if the plate type has flat bottom wells Select Strips Select if the number of strips or individual strips can be selected Mode The selection of which operating mode is to be used plate or strip Proc...

Страница 44: ...Well Manifold The speed of the aspirating pump Select if crosswise aspiration is to be used Crosswise aspiration is only for flat bottomed plates Plate type flat bottomed Plate type round bottomed Sel...

Страница 45: ...low or bottom C X P Y Wash other yes Dispense Overfl exit yes Volume 300ul exit yes Select the channel to be used 1 4 Select the volume of solution to be used 50 3000 l in increments of 50 l 50 400 l...

Страница 46: ...10 120 l in increments of 10 l Select next type of step Select and confirm Dispensing step 5 2 4 Process Step Soak Select required soaking time 1 999 seconds Select required shaking speed No Linear C...

Страница 47: ...defined Select if the microplate is to be aspirated at the end of the procedure Select required aspirating time 1 20 sec Select and confirm End step Confirm end of programming for this cycle Select re...

Страница 48: ...enters the programming procedure for the selected process step Note If an existing process step has been changed to End all previously defined steps after this step in this cycle have now been delete...

Страница 49: ...y manifold or 384 Well Manifold The speed of the aspirating pump Selected setting for crosswise aspiration Plate type Flat bottomed Plate type Round bottomed Selected operating mode strip or plate Sel...

Страница 50: ...eared or required option in Mode Selection menu The function Clear ALL is available within the Clear Program option This function enables you to clear all of the programs excluding those that are lock...

Страница 51: ...are Date Set the date only with the Barcode option Time Set the time only with the Barcode option Sel Manifold Set the manifold that is fitted to the instrument If the option Manifold Detection or Fil...

Страница 52: ...setting Time setting option Use h to change hour setting Use m to change minute setting Use Form to change from 12 to 24 hr format Manifold setting optional Use and keys to select fitted manifold 8 1...

Страница 53: ...ing position Use and keys to move the plate support so that the aspirating needles are in the correct position Bottomform Round exit yes Disp Pos XXX exit yes Asp Pos XXX exit yes Asp Pos Botm XXX exi...

Страница 54: ...Use and keys to move the manifold so that the aspirating needles are in the correct position for aspirating the bottom of the wells Washpos Botm XXX exit yes Set bottom washing position Use and keys t...

Страница 55: ...ate Position 1 Instrument Front Instrument Front Position to Edge Position to Centre Aspirate Position 2 Flat Bottom Wells Only Instrument Front Aspirate Bottom Position Instrument Front Instrument Fr...

Страница 56: ...lled with 2000ml of water Place all three bottles on the corresponding colored bottle holders Go to the Adjust menu and select Adj Liq Sens Liq Sens 1 exit yes XX displayed sensor value does not direc...

Страница 57: ...Liquid Sens 1 exit yes Use the and keys to set the next required sensor 1 2 or W Waste Sensor When the waste sensor is selected the following message is displayed Full W XX Y exit yes W waste sensor X...

Страница 58: ......

Страница 59: ...ing protective clothing and using approved laboratory safety procedures The manifold should be removed and thoroughly cleaned at least every 6 months or if one or more of the needles are blocked Lift...

Страница 60: ...eaned using The supplied cleaning needles accessory box Carefully push the cleaning needles into the aspirating and dispensing needles The small cleaning needle is for the dispensing needles and the l...

Страница 61: ...he manifolds are fitted to the instrument using the following procedures 1 Lift the manifold arm 2 Carefully fit the manifold onto the manifold arm and ensure that the locating pins are correctly inse...

Страница 62: ...old Dispensing Tube Connector Aspirating Tube Connectors Manifold Type Indicator Locating Pin Locating Pin red blue 384 Well Manifold Fig 7 4 Rear View of the 384 Well Manifold Dispensing Tube Connect...

Страница 63: ...Instrument with Fill Verification Fig 7 6 Rear View of the Manifold Dispensing Tube Connector Aspirating Tube Connectors Manifold Type Indicator Locating Pin Locating Pin Aerosol Tube Connectors Ribb...

Страница 64: ...wards the right Carefully slide the plate support with the waste sink at the rear over the guide bar The plate support must be slightly tilted so that it goes over the pin that activates the automatic...

Страница 65: ...rd Fig 7 7 The Fuse Compartment l 0 Fuse Compartment 2 Open the fuse compartment by inserting a screw driver into the slot in the right hand side of the compartment Remove the compartment out and to t...

Страница 66: ...nt cleaning procedure for this instrument is to rinse the liquid system with distilled water before the instrument is left to stand or switched off at the end of each day The manifold should be remove...

Страница 67: ...e instrument 7 4 4 Yearly Service Engineer Required Save the customers specific programs using the Winwash software Disinfect the instrument See 7 6 Instrument Disinfection Replace all aspirating and...

Страница 68: ...ith this procedure you can use either distilled water or wash solution Confirm that the rinsing solution and the tube are in the bottle Select and confirm Soak Rinse procedure Select required channel...

Страница 69: ...e used for longer times use distilled water Confirm rinsing solution and tube in bottle Select and confirm Day Rinse procedure Select required channel 1 4 Rinse Day other yes Day CH1 exit yes Rinse So...

Страница 70: ...ed water and the manifold is lowered into it Manifold left in solution for set soak time 1 min Rinse ChX YY sec Stop Wash system rinsed for set rinse time Soaking Stop Manifold left in solution until...

Страница 71: ...ed water lab quality to flush the system If the laboratory has no specific disinfection procedure the following procedure should be used to disinfect the instrument The instrument should be disinfecte...

Страница 72: ...Pack the instrument and its accessories 10 Disinfect your hands and clean them with a mild detergent 11 Complete a disinfection certificate and attach it to the outside of the box so that it is clearl...

Страница 73: ...ocedures for biohazardous waste disposal according to country specific regulations Caution Always disinfect the instrument before disposal Pollution degree 2 Method of Disposal Contaminated Waste May...

Страница 74: ......

Страница 75: ...mory 5 Program too Big If more than 60 process steps have been defined 5 Wrong Manifold Manifold not correctly defined in Adjust Menu 6 Display and LED dark Defective fuse 7 2 No or incorrect dispensi...

Страница 76: ......

Страница 77: ...fining the Start up header for the instrument Channels Procedure for setting the number of dispensing channels that are used Skip Messages Procedure to set if the warning messages are to be displayed...

Страница 78: ...ameter settings which require special tools to be redefined Select access to programming procedure Select access to Adjust menu Set Header Set number of dispensing channels fitted 1 4 Select if warnin...

Страница 79: ...his message is to be displayed Pressshow to display message Pressskip to omit message Waste Bottle OK show exit skip Select if this message is to be displayed Pressshow to display message Pressskip to...

Страница 80: ...ons For authorized service engineers only Adjust Dispensing Pump WARNING NEVER USE THE Adj Dispens Pump OPTION FOR AUTHORIZED SERVICE ENGINEERS ONLY Adj Prime Pos For authorized service engineers only...

Страница 81: ...ifold Cleaning 7 2 Exchanging 7 3 Removal 7 1 O Option 384 Well Manifold 4 9 Drip Mode 4 9 P Plate Mode 2 10 Plate Type 6 3 Priming 4 3 Program Clear 5 8 Editing 5 6 Show 5 7 R Rinse Modes Auto 7 12 D...

Страница 82: ......

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