SynOptics SYNBIOSIS aCOLyte3, Руководство пользователя

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Getting started
Introduction
aCOLyte3 is a combined hardware and software system for automatically counting colonies on
plates.
Once the system has been set up, all you have to do to count the colonies is to put each plate
on the plate holder and press a button. The counting of each plate takes no more than a few
seconds and the results are shown instantly in a table.
aCOLyte3 allows you to enter plate identifiers and dilutions manually, to use auto-
incrementing plate identifiers or to enter lists of plate identifiers and dilutions within
aCOLyte3 or to import them from a CSV file. If you use auto-incrementing plate identifiers or
a plate list, aCOLyte3 will prompt you for which plate to insert next. The plate identifiers and
dilutions are then automatically assigned to the results and recorded. You can also read plate
identifiers and dilutions using a barcode reader.
When you are counting colonies, you can use aCOLyte3:
l
to apply sophisticated algorithms to distinguish touching colonies;
l
to add or remove colonies manually after an automatic count – you can also perform a
completely manual count if required;
l
to define ‘exclude regions’ on the plate if there are any problem areas on an individual
plate where colonies cannot be distinguished for some reason – the missing area is then
automatically allowed for in calculating the total count for the plate.
All counting results are displayed and saved automatically, and any manual changes you
make to a result are marked against the individual result with comments and coded flags. By
default, an image of the plate is automatically saved with each result, and can be reloaded at
a later time for auditing. You can also view a full audit history for any changes you have made
to a result.
Once you have completed the counts/measurements, you can compile a report showing the
results.
This introduction has only mentioned a few of the features that make aCOLyte3 such a
powerful but easy tool to use. The rest of the Manual gives full instructions for using all of its
many functions.
Structure of the Manual
Note The cross-references in this manual assume that it is printed or viewed with odd numbered
pages on the right-hand side. If you are using Adobe Acrobat to read this manual on-screen,
you can display two pages side-by-side by choosing Two Page View from the View
® Page
Display submenu, and you can then choose Show Cover Page in Two Page View from the
same submenu to display the odd numbered pages on the right-hand side.
aCOLyte3 User Manual 1