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WORKING DISTANCE – the distance from the lens of the objective to
the cover slip on the slide, when the specimen is in focus.
USING YOUR SWIFT M6000 SERIES MICROSCOPE
Once you have learned the terminology and purpose of each
component of the microscope, use of the microscope is simple and
enjoyable. By following these easy steps, you will be able to begin
studying the specimen quickly and easily.
1.
Place a slide on the stage and secure it in place using the spring
loaded stage clips. If the microscope is equipped with a
mechanical stage, open the specimen holder of the mechanical
stage by pressing the finger clip lever. Carefully place the slide
against the stationary side and back edge of the mechanical stage
and release the finger clip lever.
2.
Align the specimen under the objective lens. If the microscope
has a
a mechanical stage, use the adjustment knobs to center the
specimen into the optical path. The knobs allow for the up/down
and left /right movement of the slide.
3.
After securing and moving the slide into position, turn the power
switch on. Rotate the nosepiece to place the lowest power
objective (4XD) into position over the specimen. Be sure the
objective “clicks” into position. The iris diaphragm should be
adjusted at this time to about a ¼ inch (5 mm) open.
4. While viewing through the eyepiece, rotate the coarse focus knob
slowly and carefully to bring the specimen into focus. The
specimen may require some centering in the field of view at this
time. By using the fine focusing knob, slowly and carefully refine
the focus to clearly observe the fine details of the specimen. Now
you can turn the nosepiece to the higher magnification objectives.
The objectives are parfocalized so that once the 4x objective is
focused, only a slight turn of the fine focus is required in changing
to higher power objectives.
5. Please note that smaller apertures of the diaphragm increase
contrast in the image while large apertures decrease the
contrast.
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(The diaphragm is not intended for controlling the brightness of
the illumination). A good procedure to follow in selecting the
proper
opening is to start with the largest and reduce it until the fine
detail
of the specimen is in exact focus. Using an inappropriate aperture
results in a “washing out” of the image. Care must be exercised not
to reduce the aperture too much to gain high contrast, as then the
fine structure in the image of the specimen will be destroyed.
Reducing the aperture does increase contrast and depth of focus, but
it also reduces resolution and causes diffraction. The aperture for the
10X objective will not be the same as for the 40XRD objective, since
the angle of the required light is determined by the numerical
aperture (N.A.) of the objective, the proper aperture of the
diaphragm must be selected. This can be easily achieved after
minimal experience with the microscope.
OIL IMMERSION (Only for M6003CL models)
It is desirable to use immersion oil with the 100XRD objective. Using
oil slightly increases the resolution and brightness of the image being
viewed through the microscope. Drop a tiny amount of oil onto the
slide prior to focusing with the 100XRD objective (between the slide
and the objective tip). It is essential to thoroughly clean the objective
tip after use. Please contact Swift Optical or your authorized Swift
dealer for the appropriate immersion oil to use.
IMPORTANT: The working distance of the 40XRD and 100XRD
objectives to the slide surface is very close and although the 40XRD
objective on the M6000 Series is sealed to prevent immersion oil
contamination, it is a good practice to avoid dragging the 40XRD
objective through an oiled slide.
Содержание M6001CL
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