Sutter Instrument P-97, Инструкции по эксплуатации

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A unique feature of the P-97 is its capability to loop through a program. This is demonstrated
with the multicycle Program 1, which is factory pre-programmed to pull a patch type pipette
with a tip diameter of about 2mm. Press RESET to exit Program 0, and then press < 1 >
followed by < ENTR > to enter into Program 1. The display for Program 1 should read
similar to the following:
UNLOCKED P=500 JAN 18, 1997 2:35 PM
1 1 HEAT=525,PULL= 0,VEL.= 30,DELAY= 1
Figure 3-8. Sample program.
Adjust the HEAT in the program to the Ramp value. Load glass into the puller and press
PULL. The heating filament should cycle on and off repeatedly. When the pull is complete,
the display should read similar to the following:
THE PROGRAM LOOPED 03 TIMES. THE LAST
LINE USED WAS 01, HEAT ON SEC. = 12.3
Figure 3-9. Pull cycle report (multiple-loops)..
After the heat turns on in line 01, the glass heats up and draws apart until it reaches a
VELOCITY of 30, at which point the heat turns off and the cooling air turns on. The puller is
“aware” of the fact that the glass has not separated yet, and will go back to line 01 of the
program and try again; in effect, it begins ‘looping’. It will continue to do so until the glass
separates. This looping capability is particularly useful for fabricating patch pipettes, which
require multiple heating cycles to form the characteristic stubby geometry.
3.4.2 Notes on Program Operation
There is always the possibility that the puller will be given a set of values that ‘stall’ its
operation. For example, the HEAT value has not been set high enough to melt the glass, thus
the glass cannot be pulled and no VELOCITY can be achieved. If it appears that a situation
of this type has arisen, press the STOP key. This action aborts program execution and allows
editing to take place.
All programs entered into memory (maximum of 100) remain there even after the power is
turned off or the RESET switch is toggled. A special memory ‘chip’ that carries its own
battery back up will retain stored information for as long as ten years without power being
applied to the instrument. Miracle that this is, it is strongly suggested that you keep a
written record of your programs in case of unexpected difficulties.
3.5 Parameter Adjustment
3.5.1 General Information
Micropipette and microinjection needle programs are sufficiently different from patch pipette
programs that the following information on parameter adjustments has been divided into
three sections: Micropipette/microinjection needle fabrication, Patch pipette fabrication and
Technical Tips. Even if your research only requires one type of pipette, we recommend that
you read all three sections for full appreciation of the capabilities of the puller. Please note
that the programs referred to in the following text are not necessarily meant to pull
functional pipettes, but are intended as an exercise to help develop an understanding of the
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)