Страница 1: ...OPERATOR S Instruments I J 9 NEI4I1 ...
Страница 2: ...areathatis free of dust corrosive gasesandseverevibrations In addition there should be no obstructions thatcould hinder the flow of air underand aroundthe instrument Table2 1 Cabling requirementsfor SPECTRON1C1 20 series ofspectrophotometers 200 20D DEVICE 20 20 333174 Patch Cord 333174 Patch Cord Analog Recorder 333174 Patch Cord 333132 Serial Interface Cable Kit Notavailable Accessory Printer 33...
Страница 3: ...ion for the selectedwavelength not required for SPECTRONIC 20 4 Adjust the meter to O T with the Power Switch Zero Control knob onthe front left sideof instrument Make surethe sample compartmentis empty andthe coveris closed 5 Fill a cleancell with water or blank solution and wipe the cell with a tissue to remove liquid droplets dustandfingerprints 6 Place the cell in the sample compartment and al...
Страница 4: ... the flashing reading is 1999 turn the 100 T OA control counterclockwise until the display operates normally If the flashing reading is 1999 turn the 100 T OA control clockwise until the display operates normally 1 Turn on the insn umentby turning the Power Switch knob onthe left sideof insn ument clockwise Allow the spectrophotometerto warm up for at least 15minutesto stabilize 2 After the warmup...
Страница 5: ...sthe concentrationof the standardsolution 1 Determine the factor value by following steps I through 4 of the procedure for Concentrationmode above 2 Press the MODE control key until the lED beside factor is lit 3 Press the INCREASE or DECREASE key until the desired factor is displayed a value between 0 100 and 1000 4 Pressthe MODE control key to selectthe CONCENTRAllON mode 5 Rinse and fill the ce...
Страница 6: ...vice maysendthe commandslisted in Table 2 4 to the SPECTRONICZ 20D and 20D spectrophotometer Table 2 3 lists conventions for serial I O data Table2 3 Serial fa dataconventions Wordlength Parity Echo Tenninators 8 databits I stopbit most significant bit setto 0 None None Ignores all carriage return or line feed characterssentby external device except for CR in Auto Baud Ratemode TransmitsASCn CR LF...
Страница 7: ...inter or received from an external device at a rateof 1200baud if the instrumentis turned on with the PRINT line high at logic I greaterthan 2 0 VDC Other baud rates maybe selectedwhen the spectrophotometeris connectedto a computer Theseratesinclude 110 300 1200 2400 4800 and 9600 baud If the spectrophotometeris turned on with the PRINT line low logic O lessthan 0 8 VDC it adjuststo the computer s...
Страница 8: ...in accordancewith the instruction manual maycauseinterferenceto radio communications It has beentestedandfound to comply with the limits for Class A computing device pursuantto Subpart Jof Part 15of FCC Rules which aredesignedto provide reasonableprotection againstsuchinterference when operatedin a commercial environment Operationof this equipment in a residential areais likely to causeinterferenc...
Страница 9: ... lamps glassware and similar expendablecomponents However suchparts and components may bewarranted by their manufacturer Spectronic Instruments is not responsibleunder this warranty for loss in operatingperfonnance due to environmentalconditions THIS WARRANTY IS IN LIEU OF ALL WARRANTIES EXPRESSED IMPLIED OR STATUTORY INCLUDING BUT NOT LIMITED TO WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE OR M...
Страница 10: ...ent Setup Installation Technique Sample Measurements Printing Remote operation 3 1 3 1 3 2 3 2 3 3 3 4 3 6 3 6 Section 3 Maintenance Lamp Replacement Cleaning the sample compartment Wavelength Calibration Check Photometric linearity check Replacing phototubes Service Procedure Troubleshooting Section 4 Accessories A 1 A 1 A 1 A 1 A 2 AppendixA ChoosingaReadoutMode Transmittance Mode Absorbance Mod...
Страница 11: ...ECl RONlCi 20 and SPECl RONlCi 20D Spectrophotometers SPECTRONIC 20D SPECTRONIC 20 SPECIFICA nONS 20nm Specb alslit width 340to 950nm Wavelen t 2 5nmnear525nm Wavelength accur y LED T A C Factor Wavelenj th Display Meter 51 2 mirrored scale linear T non linear A 0 to 100 T 0 to 1 95A 0to 1999C 0 1 to 1000Factor 0 to lOO T Oto2A Photomenic range j 2 T Photometric acc to 5 T Photometric noise O 5 T ...
Страница 12: ...20VAC 240VAC lOOVAC 60Hz SO 60Hz SO 60Hz SO 60Hz 1 0Amp 0 5 Amp 0 5 Amp 1 0Amp Installation CategoryII PollutionDegree2 Operating environment The instrument meetsthe specifications onthe previous page underthe following conditions aftera 30 minute warm up period Your insb ument packageincludes SPECTRONICC8 20 Cat No 333182 or SPECTRONICC8 200 Cat No 333183 spectrophotometer SPECTRONIC 1 20 SeriesS...
Страница 13: ...The SPECTRONICOII20 spectrophotometer Cat Nos 333182 000 333182 02 333182 04 333182 05 or 333182 07 shown in Figure I I is a single beam 2 3 1 I 4 6 5 Figure I I SPECTRONIC 20 spectrophotometer KEY 1 Sample compartment 2 Pilot lamp 3 Wavelength control 4 Transmittance Absorbance control lOO T OA 5 Power switch Zero control 6 Filter lever ...
Страница 14: ...ith a wavelengthrangeof 340 nmto 950 nm The nominal specb alslit width of 20 nm is constantover the entire range 7 5 6 3 4 2 81 9 1 9 10 11 Figure 1 2 KEY PECI RONIC 20D spectrophotometer I Samplecompartment2 Digital readout 3 Modeindicators4 Modeselection 5 Decrease6 Increase 7 Print 8 Wavelength control 9 Transmittance Absorbance control 100 TlOA 10 Power switch Zero Control11 Filter lever ...
Страница 15: ...the instrument are shown in Figure 1 3 g g D 0 j I d Bonomview ofSPECTRONIC 20 or 20V Figure 3 KEY 1 Lampaccess doorwiththumbscrew2 Filterlever3 Analogoutputjack SerialI O port4 line voltageswitch underthisplate international modelsonly ...
Страница 16: ...indicate thatthe 340 599nm filter should be used All gradationsare in 5 nm intervals Operating features of the SPECTRONIC 20D spectrophotometer The maincontrols for routine operationarethe Power SwitchfZero Control Wavelength Control Filter Lever Transmittance AbsorbanceControl the MODE selectorand Factor Adjust controls The accessoryAnalog Output Serial I O port is located on the undersideof the ...
Страница 17: ...to the labels TRANSMnT ANCE ABSORBANCE CONCENTRATION and FACTOR indicatethe MODE currently active AnalogOutput This port is usedto connectananalog recorderto the instrument The analog output signal level is approximately I VDC atIOO T This output is not adjustable Transmittance Absorbance Control SerialPort This control setsthe displayto lOO T O OA whena cuvettecontaining a blank reference solutio...
Страница 18: ... large openings in the lampflange with the locating pins Pressthe lamp andthe lamp sockettoward each other and rotatethe lamp clockwise until secure Donottouch thelampwithyour fingers 7 Cleanthe lamp of fingerprints and oils close the door and tighten securely This is essential for proper operation I Turn off and unplug the instrument 2 Tilt uptheunitandsetonitsback 3 Loosen thethumbscrew onthelam...
Страница 19: ...an appropriate cleaning solvent 8 Visually inspectthe lamp photodiode or phototube andfilters to determine if anyliquid hasspilled on them Refer to Figure 3 2 or Figure 3 3 9 Remove anyliquid spilled onthe lamp filter photodiode or phototube surfaces Lamp filter and phototube surfaces Clean with a soft cloth or the softestareaof a cotton swabandglass cleaner Photodiode The photodiode is easily dam...
Страница 20: ...isplay 10 Repeatsteps4 through 9 at505 510 515 and 520 nm The instrumentis in proper calibration when minimum transmittance maximum absorbance occurs between505 and 515 nm The specific transmittance or absorbance valuesare unimportant Wavelength calibration adjustment If the wavelength accuracyis out of tolerance refer to theService Procedure section onpage3 5 Customer recalibration is notrecommen...
Страница 21: ...ndsetonitsback 3 Loosen thethumbscrew onthelampaccess door seeFigure1 3 4 Remove thephototube usingtheplasticstraps asan aid seeFigure3 3 Values should be within O O2Aof the expectedabsorbancevalues if the solutions have beenpreparedcarefully Note Donottouch thelampwithyour fingers Replacing phototubes SPECTRONIC 20 or20D models only 5 Install the newphototube If a filter is required insert it int...
Страница 22: ...SPECTRONIC 2or series Maintenance g I r 0 J 1 Figure 3 3 Lamp compartmentfor SPECTRON i 20 and 20D models KEY I Phototube 2 Plastic straps 3 Filterholder 4 Lamp ...
Страница 23: ...n with at least three inchesof shockabsorbing material to prevent trapsit damage Table 3 1 Operator s Troubleshooting Guide outlines somediagnostic techniquesthat may help you isolate the causeof a problem Table3 1 Operator stroubleshooting guide I Problem II Instru nt doesnotfunction a Power line cocdnol connectedto outlet D Dead wer outlet Internal fuseblown d Defective electronic co nent a Samp...
Страница 24: ...in wavelength calibration j Defective electronic component k InCOlTectphototube ocfilter installed Replace asrequired Checkcalibration Refer to service manual X servicecenter CheckTable2 2 page2 2 roccocrect phototube SPECTRONIce20nODonly andfilter andinstall 5 Readings arenotreJx atable eventhoughtheO Tand 100 T readings areset cmectly Looselamp Tighten thumbscrew on lamp accessdOCX Loose sampleh...
Страница 25: ...ry Printer RS 232 CSerialInterfaceCable 333177 Connects SPECTRONIC 200 to Accessory Printeror computer VernierSoftware 333192 02 For using the SPECTRONIC 20D or 20D with an IBM compatible computer includes standardcurve wavelength scanand kinetics programs seeTable 2 1 on page2 1 for required cable RS 232CCable 333132 Cable Kit to connectSPECTRONIC 200 or 200 to an IBM compatible computer or SPECT...
Страница 26: ...ophotometers PICTURE DESCRIPTION CATALOG NUMBER 331709 I PATH LENGTH ADAPTER ALSO REQUIRES Square Optical 45mm 10mm 333178 included with _instrumeB 333176 Cell Holder ro 331701 11 66 mm 333178 included with instrument Cuvette Optical Glass 100 mm tall Semi Micro Cuvette 331713 10mm 333178 included with instrument 333176 I Cell Holder I included with 331713 cuvettes 1 2 Test Tube Optical Glass 100 ...
Страница 27: ...e standardadapter Cat No 333127 accommodatedonly the 1f2 diameter testtube To identify the standardadaptereasily turnthe adapter bottom end up and look into the barrelof the adapter Figure4 1 illustrates the pre 1986 designwhich will not accommodatea Y2 squarecuvette This can bereplaced with a current adapter Cat No 333178 to accommodateY2 square cuvettes Figure4 1Standardadapter KEY I Spring foun...
Страница 28: ...hese points The concentration of unknown samplesmay thenbe determined by locating the concentration value which correspondsto the percenttransmittance of the unknown on the standardcurves Absorbance measurements areusefulfor kinetics studiesandfor reactionsystems whichdo notobey Beer sLawandthereforehavenon linearstandard plots ConcentrationMode Absorbance Mode The SPECTRONICIII20D provides a more...
Страница 29: ...CTOR LED lights and note the factor on the digital display Concentration Measurements usingFACTORmode onpage2 4describes howto usethefactormode For moreinformationon concentration measurements and othertechniques contactSpectronic Instruments ApplicationLaboratoryandrequest a listof available SpecTech Notes A change in the factor indicates a change in the slope of the standardcurve due to variatio...
Страница 30: ... Q c a s z w cn Z J cn w c z z w I I I I I I I cn z W J cn z W J I I I I I z W J f I ffi I I II I I I I I I I n I t J cn t x w u w 0 11 ct wI zcn Ww U cn W LL I Z W a o 1 00 wO J ml 0 0 a o 0 r a 0 t W t W Q 0 0 I D E t t b t 0 u E 8 ...
Страница 31: ...onic com SPECTRONIC INSTRUMENTS INC EUROPE 93 96 Chadwick Road Astmoor Industrial Estate Runcorn Cheshire W A7 1PR England Telephone Int 44 1928 562522 Facsimile Int 44 1928 562529 THERMO CHINA 3 5B Asia Games Garden No 12 Xiaoying Dong Lu Chaoyang District Beijing 100101 China Telephone Int 8610 64974945 Facsimile Int 8610 64974946 For Asia Pacific and Latin America sales offices contact Rocheste...
