Spectro-UV SPECTROLINKER XL-1000 Скачать руководство пользователя страница 12 | Manualshive

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Содержание SPECTROLINKER XL-1000

Страница 1: ...1 OPERATOR S MANUAL Microprocessor Controlled UV Crosslinkers 9 20 AM05048 4 Printed in U S A XL 1000 and XL 1500 TM ...

Страница 2: ...2 LAMP CONTAINS MERCURY Manage in Accordance with Disposal Laws See www lamprecycle org ...

Страница 3: ...ODES on pages 5 7 WARNING The Spectrolinker was designed exclusively for the purposes described in this manual Never use this equipment in any manner not specified in these instructions because the protection provided by the equipment may be impaired The unit is factory calibrated at 254nm unless the customer specifies otherwise when ordering If the user wishes to change the Spectro linker to a di...

Страница 4: ...es after the mode and value are entered 7 Reset Clears all previously entered instructions and returns the Spectrolinker electronics to the WAKE state 8 Numeral Pads Selects desired time or energy level This must be done after mode selection 9 X100µJ cm2 Lit LED indicates ENERGY or OPTIMAL CROSS LINK mode operation 10 Seconds Lit LED indicates TIME mode operation 11 µW cm2 Lit LED indicates INTENS...

Страница 5: ...rrors or accidental entries Press RESET at any time to abort any operation or to correct an error The function must be selected before the value is entered If the value is entered first it will have to be re entered after the function is selected NOTE We recommend a five minute warm up period from a cold start before using the unit This allows the UV tubes to stabilize for more accurate operation ...

Страница 6: ...TART to begin operation After the dosage is delivered the Spectrolinker beeps four times and the display flashes END Open the door and the display will oscillate between DOOR and 0 To repeat the previous operation just press START after you close the door The Spectrolinker remains in the OPTIMAL CROSSLINK mode and displays 1200 i e 120mJ cm2 Otherwise press RESET to return the electronics to the W...

Страница 7: ... prior to treatment with a restriction enzyme The formation of dimers at or near the recognition site inhibits cleavage 5 4 Time Mode When this mode is selected the SECONDS LED lights to indicate that the value displayed or to be entered represents seconds Enter the exposure time desired to a maximum of 9 999 seconds 2 hours 46 minutes and 39 seconds The minimum value that can be entered is one se...

Страница 8: ...Spectrolinker and wipe dry If necessary moisten a cloth with soap and water and clean the unit Do not use any harsh chemical cleaners Do not attempt to clean the unit while it is plugged into a power source CLEANING PROCEDURE TESTING AND SCREENING RecA The Spectrolinker can be used for testing and screening RecA mutation which can prevent repair of UV induced damage on cells thereby retarding thei...

Страница 9: ...ontrolled UV crosslinkers are factory calibrated for monitoring the intensity inside the chamber to provide years of trouble free operation However should problems eventually develop with regard to intensity measurements as may occur with all light meters the unit should be returned to Spectro UV for recalibration Be sure to contact the Customer Service Department at Spectro UV for shipping instru...

Страница 10: ... and serial numbers of the unit and the date of purchase Spectro UV will make available on request circuit diagrams and component parts lists for any model unit Different wavelengths may be specified when ordering Series models without a letter are 254nm UV C Series models with the letter A are 365nm UV A and Series models with the letter B are 312nm UV B ENVIRONMENTAL CONDITIONS The Spectroline U...

Страница 11: ...Church W Gilbert Proc Nat l Acad Sci 81 1991 1995 1984 2 E W Khandjian Biotechnology 5 165 167 1987 3 E W Khandjian Mol Bio Rep 11 107 115 1986 4 D Vollrath R W Davis Nucl Acid Res 15 7865 7876 l987 5 P A Whittaker E M Southern Gene 41 129 134 1986 6 J Sambrook E F Fritsch T Maniatis Molecular Cloning A Laboratory Manual Cold Spring Harbor Laboratory 2nd Edition 1989 7 R R Reich H D Anderson Steri...

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