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Spectradyne nCS1, Руководство по эксплуатации

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© 

Spectradyne LLC, All rights reserved

 

28

 

Version 2.5.0 Revision 3, July 2020 

 

 

 
Single-particle Views: Parametric Scatter Plots 

➢

 

The  “Scatter”  button  in  the  Data  Visualization  tab  generates  a  scatter  plot  of  particle 

diameter  vs  the  parameter  selected  in  the  dropdown  list  (e.g.,  transit  time)  for  each 
detected particle event.   

➢

 

The plot mode selected in the QuickView Processed Data frame determines whether data 
from a single Stats file or multiple Stats files will be displayed.  

 

 

 

Figure 16. 

Inspecting individual peaks detected in the raw data.

 

Note: Refine Data Group 

Any outlier Stats files should be eliminated from the loaded file list at this point in 
the analysis workflow.   

➢

 

Generate a CSD plot in multi-mode to show the CSDs for all Stats files in the 
Currently Loaded list.  

➢

 

CSDs  corresponding  to  measurements  of  the  same  sample  should  be 
consistent with each other

—

any outliers should be removed from the Currently 

Loaded list using the red “X” button below the list.   

 

Содержание nCS1

Страница 1: ...Spectradyne nCS1 TM Operation Manual Spectradyne LLC www NanoparticleAnalyzer com...

Страница 2: ...the Analyte into the Cartridge 7 Load the Cartridge into the nCS1 7 nCS1 Control and Data Acquisition Software 9 Software Startup 9 Enter Run Details 9 Initializing the Cartridge 10 Single click to Ac...

Страница 3: ...a Analysis 19 After Data Acquisition 20 End Measurement Run 20 Prepare the Next Cartridge for Analysis if continuing with measurements 20 Remove the Used Cartridge 20 Insert the Next Analysis Cartridg...

Страница 4: ...the Diameter and Concentration Scaling of the CSD 29 2 2 Filter the Set of Detected Particles to Exclude False Positives 31 3 Combine and Compare 34 3 1 Combine multiple Stats files into a single Com...

Страница 5: ...rement RMM techniques A clean disposable cartridge targets a specific size range of particles for each analysis eliminating concerns of contamination between samples Specifications Dimensions 12 x 12...

Страница 6: ...t USB connection Use the USB cable provided with the instrument to connect the nCS1 to a computer with Spectradyne software installed The USB port is located on the back panel Power on Turn on the ins...

Страница 7: ...3 IMPORTANT Ensure that any air bubbles in the sample are acceptably positioned in the reservoir see Figure 2 Gently tap the cartridge on a flat surface to shift remove any bubbles if necessary Figur...

Страница 8: ...he button ring light blue on the front panel of the instrument must be illuminated before proceeding Press the cartridge engage button on the front of the nCS1 to raise the cartridge into the instrume...

Страница 9: ...Box Number First six digit date code printed on the cartridge box e g 190915 NN Save Location Use the folder icon to specify a location for saving data Data File Prefix Specify a prefix for all data...

Страница 10: ...rn shown in Figure 6 When the routine is complete data acquisition will begin automatically Figure 6 Run Control frame and fill monitors status Troubleshooting Tip If the priming routine encounters an...

Страница 11: ...isition Stop Point value is reached see page 18 In continuous mode each acquisition is automatically flagged for processing unless a significant number of defects are detected in the data see page 18...

Страница 12: ...eline Drifting of the baseline is acceptable In some situations there may be occasional steps in the baseline These steps may be acceptable depending on their severity If the raw data shows too many s...

Страница 13: ...e event of a blockage Press the Clear Constriction button Figure 9 and wait for the routine to complete Continue acquisitions as normal press Continuous on Acquisition panel How much data is enough Th...

Страница 14: ...efrigerate running buffer if intended for future use o Add 25 50 mL of deionized water to running buffer bottles or separate DI water bottles and replace in the instrument Note Use only fresh deionize...

Страница 15: ...pectradyne technician P1 IN port must always have the higher pressure setting than the rest of the ports To set the pressure at a specific port enter a PSI value max 10 psi into the corresponding entr...

Страница 16: ...onding boxes see Figure 11 Click ON SET to apply the setting Check the bias slider to ensure balance Press the OFF button to turn off the voltage and turn on the fill monitor sensors Sensors IMPORTANT...

Страница 17: ...and store it in a safe place for future use Drain Bottle Lines This automatic sequence drains any remaining liquid in the lines back into the bottles Click the Drain Bottle Lines button and follow th...

Страница 18: ...ne queue for automatic processing into Spectradyne Stats files Raw data is processed using standard parameters refer to page 25 for details o Peak Detection Threshold 3 0 x the standard deviation of t...

Страница 19: ...ements on the Live CSD o Specify an integration range using the Measure Size Range box entries provided and click Set o The measured concentration for a specific size range with statistical error is d...

Страница 20: ...n of the End Run routine lower the stage by pressing the button on the front panel of the instrument and remove the cartridge The nCS1 is now ready for the next measurement run load a new cartridge fo...

Страница 21: ...support spectradynellc com Optimal sample parameters Concentration 109 particles ml below 300 nm 108 particles mL above 300 nm Calibration beads optional Add calibration beads to final concentration 1...

Страница 22: ...d processing parameters This step yields a quantitative Concentration Spectral Density CSDTM distribution 2 Refine Filter Any necessary refinement to the diameter scaling is performed e g to account f...

Страница 23: ...Spectradyne LLC All rights reserved 23 Version 2 5 0 Revision 3 July 2020 Data Viewer Software User Interface Figure 15 Overview of the Spectradyne Viewer software user interface...

Страница 24: ...of the plot options in the Raw Data Plot Parameters frame Raw display the raw data as it was collected Polished display the raw data after default signal processing PS Filtered display the raw data a...

Страница 25: ...ains all the controls for processing raw data as well as relevant measured values for the selected raw data file The default processing parameter values are appropriate for use in most cases These def...

Страница 26: ...ion tab Note Stats Files A Stats file shares the same base filename as the raw file from which it was generated with an additional extension of the form _ssNN In the extension NN is an integer that in...

Страница 27: ...weighted or volume weighted size distribution and between concentration density or absolute concentration X Axis frame X Limits Specify the x axis limits Log X Plot in log scale on the x axis Binning...

Страница 28: ...etermines whether data from a single Stats file or multiple Stats files will be displayed Figure 16 Inspecting individual peaks detected in the raw data Note Refine Data Group Any outlier Stats files...

Страница 29: ...d to generate Stats files in the Currently Loaded files list These Stats files will contain measurements of many individual particles in the same sample under the same measurement conditions and can t...

Страница 30: ...he default calibration and the user may wish to refine the diameter scaling to align known features of the CSD e g a peak of calibration particles with the appropriate diameter or concentration Proced...

Страница 31: ...applied to be effective o Under Entire State click Selected or All to apply the loaded values to a single selected file or to all files Note Refining the diameter scaling using a known feature in the...

Страница 32: ...s the baseline noise some false positives i e peaks in the raw data that do not likely represent real particles naturally are included in the Stats file data set see Figure 17 The goal of this step in...

Страница 33: ...e range of 0 2 4 0 approx Signal to noise S N o S N 15 cartridge dependent o A general sense for an appropriate S N cutoff can also be obtained by inspecting peaks in the raw data Figure 17 and Viewin...

Страница 34: ...parameter and include an infinite range of diameters Polygon Filter has 2D user defined polygonal boundaries similar to a gate commonly used in analysis of flow cytometry results o Select the filter p...

Страница 35: ...bined file File name of the form prefix _YYYYMMDD_ccNN h5 where NN is an incrementing number unique for each day o A Run Report Same file name as Combined file with xlsx extension 3 2 Compare Multiple...

Страница 36: ...0 D90 values corresponding to 10 50 and 90 of particles are available for display on the CSD In the Quantification tab of the Viewer select the size range of interest either graphically or by manually...

Страница 37: ...el xlsx format file containing a complete summary of the measurement It contains o Meta information about the measurement o Graphical size distributions in linear and logarithmic y axes o Summary stat...

Страница 38: ...Three formats are available for export Microsoft Excel format xlsx MS DOS CSV format csv Lumetics Link format lum For Excel format the file selected in the Currently Loaded files list is exported to...

Страница 39: ...Spectradyne LLC All rights reserved 39 Version 2 5 0 Revision 3 July 2020 Cartridge Size and Concentration Coverage A more up to date cartridge size coverage may be found on our website...

Страница 40: ...operation of the nCS1 and its software can be obtained by sending email to support spectradynellc com At Spectradyne we are eager to help with your particle analysis Please contact us for help with sp...

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