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Refrigerated Centrifuge Sigma 3-30KS

6 Using the centrifuge

Version 07/2012, Rev. 1.22 of 02/06/2021

• sb

43 / 93

Translation of the original operating manual, part no. 0701302

Fig. 17: Program "RAPID_TEMP"

NOTE

The program will only be loaded if the actual temperature is above the set
temperature.

• Press the start button in order to start the rapid cooling run.
• During the rapid cooling run, the set temperature can be modified within

the range below the actual temperature.

The current status of the program will be displayed on the status bar.

The

“RAPID_TEMP“ program will be stopped under the following

conditions:
• The set value is reached. In this case, the "RAPID_TEMP" program

stops with a sound signal and the standstill cooling system will be
activated.

• The stop button is pressed. The "RAPID_TEMP" program will be

stopped prematurely. No message will be issued when the set
temperature is reached.

• A parameter is changed (except for the temperature) or another input is

made. In this case, the "RAPID_TEMP" program will be aborted. No
message will be issued when the set temperature is reached.

After the stop, the previous program will be reloaded or the changed
parameters will be adopted as the new settings.

NOTE

The automatic lid opening function is suppressed after a rapid cooling run
in order to prevent the system from reheating.

NOTE

The delta T temperature monitoring system (see chapter 6.3.2.7 -
"Parameters menu", Process) remains inactive as long as the
"RAPID_TEMP" program is active.

Содержание 3-30KS

Страница 1: ...ation of the original operating manual sb 07 2012 Rev 1 22 of 02 06 2021 Sigma 3 30KS part no 0701302 Sigma 3 30KS from serial no 146129 Refrigerated Centrifuge Operating Manual Please retain for late...

Страница 2: ...Refrigerated Centrifuge Sigma 3 30KS 2 93 Version 07 2012 Rev 1 22 of 02 06 2021 sb Translation of the original operating manual part no 0701302...

Страница 3: ...021 sb 3 93 Translation of the original operating manual part no 0701302 Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 Fax 49 0 55...

Страница 4: ...Refrigerated Centrifuge Sigma 3 30KS 4 93 Version 07 2012 Rev 1 22 of 02 06 2021 sb Translation of the original operating manual part no 0701302...

Страница 5: ...gal force 15 2 2 2 2 Density 15 3 Safety 16 3 1 Marking of the unit 16 3 2 Explanation of the symbols and notes 17 3 3 Responsibility of the operator 18 3 4 Requirements concerning the personnel 19 3...

Страница 6: ...f a standard angle rotor with a hermetic lid 33 6 2 2 3 Installing a rotor with the g lock system 34 6 2 2 4 Installation of accessories 34 6 2 2 5 Adapters 36 6 2 2 6 Vessels 36 6 3 Control system Sp...

Страница 7: ...ors buckets and carriers 66 8 1 4 Load bearing bolts 67 8 1 5 Glass breakage 68 8 2 Sterilisation and disinfection of the rotor chamber and accessories 68 8 2 1 Autoclaving 69 8 3 Service 70 8 4 Retur...

Страница 8: ...Refrigerated Centrifuge Sigma 3 30KS Table of contents 8 93 Version 07 2012 Rev 1 22 of 02 06 2021 sb Translation of the original operating manual part no 0701302...

Страница 9: ...ed use Centrifuges are power driven machines that separate liquids from solid matter liquid mixtures or solid mixtures by centrifugal force They are solely intended for this purpose Any other use beyo...

Страница 10: ...ny other way Non compliance may be prosecuted under criminal law 1 5 Standards and regulations These operating instructions have been created in accordance with the relevant European standards and reg...

Страница 11: ...ation of the original operating manual part no 0701302 2 Layout and mode of operation 2 1 Layout of the centrifuge 2 1 1 Functional and operating elements 1 Lid 2 User interface see chapter 6 3 1 User...

Страница 12: ...12 93 Version 07 2012 Rev 1 22 of 02 06 2021 sb Translation of the original operating manual part no 0701302 Fig 2 Rear view of the centrifuge 220 240 V Fig 3 Rear view of the centrifuge 200 V and 208...

Страница 13: ...2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5 Serial number 6 Part number 7 Type 8 Nominal voltage 9 CE mark in compliance with the directive 2006 42 EC 10 Sy...

Страница 14: ...on of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increase Das...

Страница 15: ...ula If afterwards the speed or the radius is changed the resulting relative centrifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted whi...

Страница 16: ...led Adhesive label concerning the circuit breaker Name plate see chapter 6 2 2 4 Installation of accessories CE mark in compliance with the directive 2006 42 EC Do not dispose as part of domestic wast...

Страница 17: ...problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of these symbols causes serious healt...

Страница 18: ...as with the accident prevention regulations must be checked at regular intervals e g every month Under the international rules for health and safety at work the operator is obliged to take measures i...

Страница 19: ...nger zone or work area This manual uses the following personnel qualifications for various areas of activity Qualified electrician Due to their special training knowledge experience and familiarity wi...

Страница 20: ...The operating manual must be kept at the location of use of the centrifuge Ensure that it is accessible at all times The operating manual must be handed over to any subsequent owner or operator of th...

Страница 21: ...be removed Inspect the electrical equipment of the unit regularly Defects such as loose or burnt cables must be eliminated immediately Following the completion of any type of repair or service the qua...

Страница 22: ...n the centrifuge when it is not in use so that moisture can evaporate 3 6 3 Fire prevention DANGER Do not spin explosive or inflammable substances Do not use the centrifuge within hazardous locations...

Страница 23: ...n against the use of equipment of poor quality Breaking glass or bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 I...

Страница 24: ...of safety If other data concerning the service life are engraved on the rotor or bucket these data shall apply accordingly For example a bucket with the engraving max cycles 10 000 has a service life...

Страница 25: ...signals with regard to plausibility Errors are detected with extreme sensitivity and displayed as error messages in a dialog box see chapter 7 2 Table of error codes 3 7 4 Earth conductor check An ea...

Страница 26: ...g hazards The centrifuge was built in accordance with the state of the art and in compliance with the generally recognized safety rules However danger to life and limb of the operator or of third part...

Страница 27: ...year Store the centrifuge only in dry rooms The permissible storage temperature is between 20 C and 60 C If you would like to store it for more than one year or if you intend to ship it overseas plea...

Страница 28: ...ge 4 5 Transport safety device The transport safety device consists of a screw which is located at the bottom panel CAUTION The transport safety device must be removed prior to start up because the sc...

Страница 29: ...solid even surface Attention During transport from cold to warmer places condensational water will collect inside the centrifuge It is important to allow sufficient time for drying min 24 h before th...

Страница 30: ...lly centrifuges with a nominal voltage of 220 240 V must be protected with 16 Amp B fuses that are to be provided by the customer Centrifuges with a nominal voltage of 200 V or 208 V must be protected...

Страница 31: ...ction 6 2 Switching the centrifuge on Press the mains power switch The display then illuminates The centrifuge is ready for operation 6 2 1 Opening and closing the lid The lid can be opened if the cen...

Страница 32: ...ench with 7 5 Nm In doing so hold the rotor at its outer rim Follow the safety instructions and hazard warnings see chapter 3 Safety WARNING Once a day or after 20 cycles the rotor tie down screw must...

Страница 33: ...r shaft Insert the rotor tie down screw into the motor shaft Tighten the rotor tie down screw clockwise with the supplied rotor wrench with 7 5 Nm In doing so hold the rotor at its outer rim Follow th...

Страница 34: ...4 Installation of accessories Only use vessels that are suitable for the rotor In the case of swing out rotors all of the compartments must be equipped with buckets Always load opposite compartments...

Страница 35: ...s and their suspensions are loaded evenly It is not permissible to load angle rotors on only one axis correct incorrect Fig 10 Permissible and impermissible loading of an angle rotor and a swing out r...

Страница 36: ...ltiple carriers cause corrosion Fill the vessels carefully and arrange them according to their weight Imbalances result in the excessive wear of the bearings Always fill the tubes up to their useful v...

Страница 37: ...display is divided into several different fields The various functions of the system can be called up by pressing and turning the function knob 1 Start button 2 Display 3 Function knob 4 Stop button 5...

Страница 38: ...before a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered Quick stop will...

Страница 39: ...2 1 Speed radius and relative centrifugal force The maximum speed values depend on the rotor that is used Relative centrifugal force RCF The relative centrifugal force is the acceleration that the sam...

Страница 40: ...ached Select the field Runtime and confirm the selection The display flashes when it is activated Turn the function knob from the time 0 00 10 anti clockwise or from the time 99 59 59 clockwise Infini...

Страница 41: ...e data Fig 15 Rotor selection list here with potential rotor bucket combinations and additional information Automatic rotor identification The centrifuge automatically identifies the rotor that is cur...

Страница 42: ...enu shows the program that is currently loaded When the field is selected the program list is displayed for information on how to work with the programs please see chapter 6 3 3 Program mode The progr...

Страница 43: ...is case the RAPID_TEMP program stops with a sound signal and the standstill cooling system will be activated The stop button is pressed The RAPID_TEMP program will be stopped prematurely No message wi...

Страница 44: ...splayed temperature will deviate from the actual sample temperature Progress indicator The progress indicator provides a quick overview of the remaining runtime of the running centrifugation run For t...

Страница 45: ...d The order of the processes corresponds to their storage locations on the program list Empty storage locations will not be displayed If more than 11 programs have been stored the user can scroll thro...

Страница 46: ...the standstill cooling function is activated the centrifuge starts to precool after it is switched on In the runtime field the symbol is displayed The lid must be closed CAUTION Unmoved air in the rot...

Страница 47: ...oring function enables the continuous monitoring of the speed and runtime parameters during the centrifugation Activate the centrifugation monitoring function by clicking NOTE If the function is activ...

Страница 48: ...urrent temperature is between the preset temperature in the Standard menu and the Delta T limit value If the temperature leaves this range during the centrifugation run an error message will be displa...

Страница 49: ...eters function is selected the Save function will also be selected Select the button Activate code Enter a four digit code and confirm the entry The lock is now active The symbol will be displayed in...

Страница 50: ...u Setup Function Open lid after run The automatic lid opening function ensures that the lid opens when the rotor has stopped CAUTION When the lid is open the cooling is not active The sample may warm...

Страница 51: ...be displayed in C Celsius or F Fahrenheit Language The control system can be used in various language versions If a language is selected by mistake it can be changed on any screen as follows Press an...

Страница 52: ...etters and characters can be entered when the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press the knob again in...

Страница 53: ...If the curve number is already used the stored curve will be displayed The input field Int is used to specify the interval number of the process Up to ten intervals can be entered for a curve Enter t...

Страница 54: ...possible speed depending on the rotor and from the slope limitation Example 1 Start speed 0 min 1 rpm final speed 100 min 1 rpm runtime 1 hour not possible since the necessary slope 0 03 min 1 rpm whi...

Страница 55: ...tains all of the data that are required for a centrifuge run Certain sedimentation results can be repeated under identical conditions Programs can be loaded executed edited and deleted when the centri...

Страница 56: ...a letter and press it to confirm the selection Then the next character can be selected Pressing the arrow button will delete the last character When the program name is complete select OK and confirm...

Страница 57: ...e desired program from the list and confirm the selection by pressing the function knob Press the start button Or Open the process library select the desired program and press the start button The pro...

Страница 58: ...aded while the program rotation function is active this program will be used as the start program for the rotation After the completion of the program the next program on the program list will be load...

Страница 59: ...tions for data input and output Connection for a serial interface depending on the centrifuge type partly standard External signal active DC 24V 0 5 A max part no 17701 Floating switch AC 250V max 6 A...

Страница 60: ...ff on If the error occurs again contact service Centrifuge cannot be started lid key LED flashes The lid lock is not closed correctly Open and close the lid If the error occurs again contact service C...

Страница 61: ...rewdriver Fig 32 Position of the opening for the emergency lid release Insert the supplied hexagon socket key horizontally into the hole The key will be guided through a funnel shaped tube to the shaf...

Страница 62: ...n Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error only for centrifuges with imbalance monitoring system A...

Страница 63: ...malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentrifugen de O...

Страница 64: ...ces Do not use solvents Do not use agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress e g by heat generators 8 1 Maintenance 8 1 1 Centr...

Страница 65: ...pecial safety measures must be considered as these are measures that will ensure operational safety at the same time Immediately rinse off the rotor buckets or accessories under running water if they...

Страница 66: ...d the permanent high stress from high gravitational fields Chemical reactions as well as stress corrosion combination of oscillating pressure and chemical reaction can affect or destroy the metals Bar...

Страница 67: ...e Load bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance Clean the load bearing bolts and bucket groove in order to remove the old grease Apply a small...

Страница 68: ...n order to completely remove the glass particles and metal dust from the rotor chamber Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a mo...

Страница 69: ...cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polyallomer polycarbonate round carriers 115 118...

Страница 70: ...ts were used during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of the...

Страница 71: ...re a declaration of decontamination The form must be filled in completely and signed by authorised and specialised personnel only Affix the original form in a clearly visible manner to the outside of...

Страница 72: ...01302 NOTE The defective part unit must be packaged in a transport safe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packag...

Страница 73: ...permissible to dispose of the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of d...

Страница 74: ...the transformer AT 1 8 12 0 16 0 at 208 V 60 Hz 16 0 at 200 V 50 60 Hz temperature fuses Performance data Max speed rpm Max capacity ml Max gravitational field x g Max kinetic energy Nm 30 000 510 65...

Страница 75: ...e of 100V or 200V a tolerance of 10 5 applies For indoor use only Allowable ambient temperature 5 C to 35 C Max allowable relative humidity of air 80 from 5 C up to 31 C with a linear decrease to 67 r...

Страница 76: ...t of accessories can be downloaded from www sigma zentrifugen de 11 1 1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respective rotor as shown...

Страница 77: ...fuge Sigma 3 30KS 11 Appendix Version 07 2012 Rev 1 22 of 02 06 2021 sb 77 93 Translation of the original operating manual part no 0701302 11 2 Speed gravitational field diagram Fig 37 Speed gravitati...

Страница 78: ...by 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maximum power The runtime until the set speed is reached depends solely on the moment of inertia of...

Страница 79: ...Centrifuge Sigma 3 30KS 11 Appendix Version 07 2012 Rev 1 22 of 02 06 2021 sb 79 93 Translation of the original operating manual part no 0701302 Fig 40 Diagram of linear curves Fig 41 Diagram of quad...

Страница 80: ...ars 1 14 1 14K 12084 7 years 1 14 1 14K 12085 7 years 1 14 1 14K 12092 5 years 20x 1 14 1 14K 12093 5 years 20x 1 14 1 14K 12094 5 years 20x 1 14 1 14K 12096 5 years 20x 1 14 1 14K 12097 5 years 20x 1...

Страница 81: ...4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride NH4 Cl aqueous 1 1...

Страница 82: ...ne C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylaniline C8H11N...

Страница 83: ...3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1 1 1 1 1 Nit...

Страница 84: ...1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4...

Страница 85: ...Refrigerated Centrifuge Sigma 3 30KS 11 Appendix Version 07 2012 Rev 1 22 of 02 06 2021 sb 85 93 Translation of the original operating manual part no 0701302 11 6 EC declaration of conformity...

Страница 86: ...Refrigerated Centrifuge Sigma 3 30KS 11 Appendix 86 93 Version 07 2012 Rev 1 22 of 02 06 2021 sb Translation of the original operating manual part no 0701302...

Страница 87: ...efrigerated Centrifuge Sigma 3 30KS 11 Appendix Version 07 2012 Rev 1 22 of 02 06 2021 sb 87 93 Translation of the original operating manual part no 0701302 11 7 Declaration of conformity China RoHS 2...

Страница 88: ...Refrigerated Centrifuge Sigma 3 30KS 11 Appendix 88 93 Version 07 2012 Rev 1 22 of 02 06 2021 sb Translation of the original operating manual part no 0701302...

Страница 89: ...ve or pathogenic substances 22 Centrifugation principle 14 Centrifugation with low capacity 35 Centrifugation with vessels of various sizes34 Centrifuge cannot be started 60 Centrifuge decelerates dur...

Страница 90: ...7 Explosive substances 22 External signal 52 F Filling quantity refrigerant 74 Fire preventions 22 Form for the return of defective parts 71 Function 50 Functional and operating elements 11 Fuses have...

Страница 91: ...tor shaft 32 33 64 Multiple carrier 36 N Name plate 11 13 No indication on the display 60 No power in the mains supply 60 Noise level 74 Nominal voltage 13 Notes on safety and hazards 9 Notes on trans...

Страница 92: ...6 Safety instructions for centrifugation 23 Safety chemical and biological 22 Safety electrical 21 Safety mechanical 21 Safety conscious work 18 Saving a program 56 Scope of supply 10 Screws of the tr...

Страница 93: ...5 Temperature monitoring 48 Temperature monitoring system 25 Temperature range 74 Temperature unit 51 Temperature value not reached 60 Thermal stress 64 Time fine 51 Time range 74 Toxic substances 22...

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