Laboratory Centrifuge Sigma 2-16P
6 Using the centrifuge
Version 04/2007, Rev. 2.23 of 28/04/2022
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Translation from the original operating manual, part no. 0700602
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6.2.2.3
Installation of a microhaematocrit rotor
• Open the centrifuge lid by pressing the lid key.
• Replace the rotor tie-down screw with the rotor tie-down screw of the
microhaematocrit rotor (part no. 81543, included in the scope of supply
of the microhaematocrit rotor), and tighten it loosely.
• Lower the rotor with its central bore straight down onto the motor shaft.
• Tighten the rotor tie-down screw clockwise with 3 Nm using the supplied
rotor wrench. In doing so, hold the microhaematocrit rotor with one hand
and tilt it slightly in order to prevent the motor shaft from slipping
through.
• Check the rotor for a correct and tight fit.
• Follow the safety instructions and hazard warnings (see chapter 3 -
CAUTION
Please consider the maximum permissible gravitational field of 12,000 x g.
An excess gravitational field will result in an increased risk of glass
breakage!
Operation
• Fill the capillary tubes with blood and seal them at one end with putty or
by fusion.
• Place the capillary tubes into the recesses of the rotor with the sealed
end against the rubber ring. Ensure that the capillary tubes fit tightly
against the rubber ring. The opposite places must be loaded.
• Put the rotor cover on and lock it.
• Close the centrifuge lid.
• Enter the following parameters: RCF 12,000 x g, runtime approx.
5 minutes.
• Start the centrifuge.
• Open the centrifuge lid when the rotor has stopped.
• Unlock the rotor cover and lift it off.
Evaluation
• Put the reader onto the microhaematocrit rotor.
• Turn the reader and perform a fine adjustment with the aid of the central
eccentric mechanism in order to localize the O-point and the maximum
liquid point in the capillary tubes. You can now read the percentage
value.
• Remove the capillary tubes. Some of the tubes can also be evaluated
with the reader outside of the rotor (see the instructions for use on the
back of the reader).
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