Shodex SUGAR SP0810 8C Скачать руководство пользователя страница 1

ver.211015E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 SUGAR

 SP0810 8C 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 

 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

 product. Shodex

 SUGAR

 SP0810 8C is a high performance 

chromatography column which is specifically designed for the separations and analysis of sugars. A strong acid 
cation exchange type packing gel, prepared from a rigid resin of styrene divinylbenzene copolymer provides a 
mixed separation mode. The analytes are separated by a combination of ligand exchange and size exclusion 
modes. It is capable of rapidly separating and analyzing monosaccharides or disaccharides from each other, 
and also sugar alcohols.       

 

 

2. Column Components   

 

                 

 

 

 
 
 
 
 
 
 
 
 
3. Column Specifications 

Product 

Code 

Product Name 

Column Size 

(mm) 

Particle size 

(

μ

m) 

Theoretical Plate 

Number 

(Per Column) 

Exclusion Limit

*

 

I.D. Length 

F6378106 

SUGAR SP0810 8C 

8.0 100 

 3,000 

1,000 

 

Reference value only / Measured with pullulan 

 

Base Material 

: Spherical porous particles of styrene divinylbenzene copolymer modified with sulfo group

(counter ion: Pb

2+

)

 

Column Housing  : SUS-316 
Screw Type 

: Internally-threaded type No.10-32 UNF 

Shipping Solvent : Water 
 
 
 

Содержание SUGAR SP0810 8C

Страница 1: ... Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex SUGAR SP0810 8C is a high performance chromatography column which is specifically designed for the separations and analysis of sugars A strong acid cation exchange type packing gel prepared from a rigid resin of styrene divinylbenzene copolymer provides a mixed separation mode The analytes are ...

Страница 2: ...have been changed absorbed moisture or has been contaminated Always use freshly prepared solvents Solvents stored for a long time may have changed their compositions and may influence elution patterns and or damage the column Note Use of on line degasser is recommended 6 Sample Preparation 1 If possible use the eluent for analysis to dissolve or dilute samples If this is difficult use a solvent wh...

Страница 3: ...the column at an elevated temperature keep a low flow rate until the temperature of the column reaches to the set temperature and then gradually increase the flow rate to the desired 4 At the end of analysis reduce the flow rate to 0 2 0 3 mL min and turn the column oven off Make sure that the column oven temperature is at room temperature before turning off the pump Caution Verify that there is n...

Страница 4: ...he lead ion attached to the sulfo functional group This can change the separation patterns and peak shapes Following regeneration procedure reattaches the lead ions and may resume the column performance Set the column temperature between 70 95 C and introduce water at 0 5 mL min flow rate Inject 50 μL of 0 2 M aqueous lead nitrate solution several times Caution Please be careful when disposing the...

Страница 5: ...d surface 3 When disposing the column please follow a proper waste disposal method specified by your local regulations Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support office https www shodex com en support_office list ...

Страница 6: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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