ver.210706E
SHOWA DENKO K.K. (https://www.shodex.com/)
2
4.
Usable Conditions
*
Room temperature
Usable solvents are listed below.
(1) The standard eluent is a mixture of water and acetonitrile, methanol or ethanol of any ratio.
(2) Buffers such as phosphate, acetate, tris-HCl and aqueous solutions of salts such as sodium chloride,
potassium chloride, sodium sulfate can be used up to 0.5 M.
Attention!
· Use the column within above stated flow rate, pressure, and temperature ranges. Using
the column outside the given range may damage the column and lower its performance.
· When using a mixture of buffer (or aqueous solution of salt) and organic solvent, make
sure there is no precipitation of salt.
· Non-polar solvents such as benzene, chloroform and hexane cannot be used.
· When using highly corrosive salts such as sodium chloride, wash out the salts at the end
of analysis. The metal parts of the devices and/or the columns may rust.
·
Column pressure is influenced by the eluent composition, flow rate, and column
temperature. When changing the eluent compositions, adjust the flow rate and column
temperature so that the column pressure remains below the usable maximum pressure.
5. Eluent Preparation
(1) Degas the eluent fully to prevent the formation of air bubbles.
(2) Presence of small debris or insoluble substances may result in deterioration of the column and/or they appear
as noise on the chromatograms. Filter the eluent with a 0.45-
μ
m disposable filter to prevent the problems.
Attention!
· Whenever water is required, use ultra-pure water freshly generated by a water purification
system or water from a newly opened HPLC grade distilled water bottle. Use HPLC grade
organic solvent whenever possible. Solvents left in an opened bottle for a long time should
not be used. The content may have been changed, absorbed moisture, or has been
contaminated.
· Always use freshly prepared solvents. Solvents stored for a long time may have changed
their compositions and may influence elution patterns and/or damage the column.
Note
· Use of on-line degasser is recommended.
6. Sample Preparation
(1) If possible, use the eluent for analysis to dissolve or dilute samples. If this is difficult, use a solvent which
has a composition that is as close as possible to the eluent's composition, but which fully dissolves or dilutes
the sample.
When gradient elution is used, it is recommended to use the initial eluent to prepare the sample.
(2) Filter the sample solution using disposable 0.45-
μ
m filter to prevent the column from clogging or deteriorating.
(3) Recommended injection volume is 50
μ
L per column.
(4) For the samples containing proteins, remove the proteins prior to the sample injection.
Note
· Use of guard column is recommended to protect the analytical column.
Product Name
Flow Rate (mL/min)
Maximum Pressure
(MPa) (Per Column)
pH Range
Temperature
Range (°C)
Recommended Maximum
RSpak DE-613
≤
2.0
3.0
7.0
2 - 12
RT
*
- 70
RSpak DE-G 4A
-
-
-
