ver.211214E
SHOWA DENKO K.K. (https://www.shodex.com/)
4
7.4 Column Cleaning
Problems in peak shapes and elution time changes or elevated column pressure etc. are often caused by
insoluble or adsorbing components present in the eluent and reagents being deposited inside the column. These
problems may be resolved by cleaning the column. When washing the column, let the washing solution flow
from the column outlet go directly into the waste container and not let the solution go through the detector.
<Cleaning Method>
(1)
Insoluble components that block the column inlet may be removed by reversing the flow direction, i.e.,
introducing the eluent from the column outlet, with flow rate at less than half of the recommended flow rate.
(2) Follow below example for washing the adsorbing components. For an efficient cleaning, reverse the flow
direction.
Introduce 50 mL of acetonitrile or methanol at 0.5 mL/min.
8. Column Storage
Remove the column from the system keeping the solvent used for the analysis. Securely tighten the end caps
and store the column at a location with stable temperature (a cool and dark space is recommended).
Attention!
· Never allow inside of the column to dry. It can damage the column.
9. Column Inspection
Inspection method is described in the Certificate of Analysis (CoA).
Theoretical Plate Number (N) and Asymmetry Factor (Fas) were calculated using the below equations.
(1) Theoretical Plate Number (N)
(2) Asymmetry Factor (Fas)
10. Additional Warnings
(1) Do not remove end fittings.
(2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface.
Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications.
For additional assistance, contact the distributor from whom you purchased the column or contact your regional
Shodex
support office (https://www.shodex.com/en/support_office/list/).
