ver.211124E
SHOWA DENKO K.K. (https://www.shodex.com/)
2
4.
Usable Conditions
Usable solvents are listed below.
(1) A mixture of water and acetonitrile or water and methanol of any ratio can be used. Up to 100 % water,
acetonitrile, or methanol can be used. For the analysis of aromatic compounds, if the theoretical plate
number obtained was significantly lower than expected when using methanol, try using acetonitrile instead.
(2) Up to 100 % ethanol, isopropanol (IPA), and tetrahydrofuran (THF) can be used.
(3)
Various buffer and aqueous salt solutions can be used instead of water. They include buffers such as
phosphate, acetate, and Tris and salts such as sodium chloride and potassium chloride. Please keep total
concentration of salts under 0.1 M. Their recommended concentration ranges are 1 - 50 mM. Acids like
trifluoroacetic acid (TFA), formic acid, and acetic acid can also be used.
(4)
Up to 2-M ammonium sulfate can be used.
Attention!
· Use the column within above stated flow rate, pressure, and temperature ranges. Using
the column outside the given range may damage the column and lower its performance.
· When using a mixture of buffer or salt solution and organic solvent, make sure there is no
precipitation of salt.
· When using highly corrosive salts such as sodium chloride, wash out the salts at the end
of analysis. The metal parts of the devices and/or the columns may rust.
·
Column pressure is influenced by the eluent composition, flow rate, and column
temperature. When changing the eluent compositions, adjust the flow rate and column
temperature so that the column pressure remains below the usable maximum pressure.
5. Eluent Preparation
(1) Degas the eluent fully to prevent the formation of air bubbles.
(2) Presence of small debris or insoluble substances may result in deterioration of the column and/or they appear
as noise on the chromatograms. Filter the eluent with a 0.45-
μ
m disposable filter to prevent the problems.
Attention!
· Whenever water is required, use ultra-pure water freshly generated by a water purification
system or water from a newly opened HPLC grade distilled water bottle. Use HPLC grade
organic solvent whenever possible. Solvents left in an opened bottle for a long time should
not be used. The content may have been changed, absorbed moisture, or has been
contaminated.
· Always use freshly prepared solvents. Solvents stored for a long time may have changed
their compositions and may influence elution patterns and/or damage the column.
Note
· Use of on-line degasser is recommended.
6. Sample Preparation
(1) If possible, use the eluent for analysis to dissolve or dilute samples. If this is difficult, use a solvent which
has a composition that is as close as possible to the eluent's composition, but which fully dissolves or dilutes
the sample. When gradient elution is used, it is recommended to use the initial eluent to prepare the sample.
(2) Filter the sample solution using disposable 0.45-
μ
m filter to prevent the column from clogging or deteriorating.
(3) Recommended sample injection volume is less than 5
μ
L per column.
Note
· Use of guard column is recommended to protect the analytical column.
Product Name
Flow Rate (mL/min)
Maximum
Pressure (MPa)
(Per Column)
pH
Range
Temperature
Range (°C)
Recommended Maximum
ODP2 HP-2B
0.1 - 0.2
0.4
12
3 - 12
20 - 60
ODP2 HP-2D
15
ODP2 HPG-2A
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