Shodex ODP2 HP-2B Скачать руководство пользователя страница 1

ver.211124E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 ODP2 HP-2B, 2D 

 

(Please read this manual carefully to achieve the best and consistent column performance for a long time) 

 
 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

 product. Shodex

 ODP2 HP series is polymer-based reversed phase 

chromatography columns which retain hydrophilic compounds stronger than ordinarily ODS columns do. The 
small pore size of the packing material excludes proteins fast, and this makes the columns suitable for 
separating small-molecular weight components, such as drugs, even in the presence of protein. The 2-mm ID 
columns, ODP2 HP-2B and ODP2 HP-2D, are especially effective for the LC/MS analysis.   
The column also works as a hydrophobic interaction chromatography (HIC) column by using high-concentration 
ammonium sulfate aqueous solution as an eluent.   
 

2. Column Components   

 
 

 
 
 

               

 

 

 
 
 
 
 
 
 

3. Column Specifications 

Product 

Code 

Product Name 

Column Size (mm) 

Particle 

Size (

μ

m) 

Theoretical Plate Number 

(Per Column) 

I.D. Length 

F7622004 

ODP2 HP-2B 

2.0  

50 

 3,000   

F7622005 

ODP2 HP-2D 

2.0 150 

 7,000   

F6714011 

ODP2 HPG-2A 

2.0  

10 

(Guard 

Column) 

 

 

Base Material 

: Spherical porous particles of polyhydroxymethacrylate

 

Column Housing  : SUS-316   
Screw Type 

: Internally-threaded type No.10-32 UNF 

Shipping Solvent : Water/Acetonitrile = 55/45 

 

Содержание ODP2 HP-2B

Страница 1: ... 1 Introduction Thank you for purchasing the Shodex product Shodex ODP2 HP series is polymer based reversed phase chromatography columns which retain hydrophilic compounds stronger than ordinarily ODS columns do The small pore size of the packing material excludes proteins fast and this makes the columns suitable for separating small molecular weight components such as drugs even in the presence o...

Страница 2: ...t the column pressure remains below the usable maximum pressure 5 Eluent Preparation 1 Degas the eluent fully to prevent the formation of air bubbles 2 Presence of small debris or insoluble substances may result in deterioration of the column and or they appear as noise on the chromatograms Filter the eluent with a 0 45 μm disposable filter to prevent the problems Attention Whenever water is requi...

Страница 3: ...nd fitting Presence of an extra space will let the sample to spread out and may result in wide peaks 3 Set the initial flow rate at less than 0 1 mL min and start the system If using the column at an elevated temperature keep a low flow rate until the temperature of the column reaches to the set temperature and then gradually increase the flow rate to the desired Caution Verify that there is no so...

Страница 4: ...the column outlet go directly into the waste container and not let the solution go through the detector Cleaning Method 1 Insoluble components that block the column inlet may be removed by reversing the flow direction i e introducing the eluent from the column outlet with flow rate at less than half of the recommended flow rate 2 Follow below cleaning steps for adsorbing components For an efficien...

Страница 5: ... Solvent Exchange for how to replace the eluent Attention Never allow inside of the column to dry It can damage the column 9 Column Inspection Inspection method is described in the Certificate of Analysis CoA Theoretical Plate Number N and Asymmetry Factor Fas were calculated using the below equations 1 Theoretical Plate Number N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fi...

Страница 6: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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